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Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae)

Yıl 2023, Cilt: 14 Sayı: 1, 76 - 83, 25.07.2023
https://doi.org/10.31019/tbmd.1278477

Öz

The rose aphid, Macrosiphum rosae L. (Hemiptera: Aphididae), is one of the important pests of roses (Rosa spp.) (Rosaceae) in many parts of the world, including Türkiye. In the present study, the pathogenic activity of five indigenous entomopathogenic fungal (EPF) isolates [three isolates of Beauveria bassiana (BbDm-1, BbKm-1, BbMp-1), one isolate of Isaria farinosa (IfGp-1) and one isolate of Purpureocillium lilacinum (PlKa-1)], were tested for their activity against nymphs and adults of M. rosae under laboratory conditions. Spore suspensions of three different concentrations (1 × 107, 1 × 108 and 1 × 109 conidia/ml) obtained from a 10-day old culture of the isolates on PDA (Potato Dextrose Agar) medium were tested by spray application against both nymphs and adults of M. rosae. The mortality rate was dose-dependent and increased with spore concentration of the isolates (P < 0.05). Of the five isolates tested, two B. bassiana isolates, BbDm-1 and BbKm-1, were the most pathogenic and caused mortalities of 100% and 83.3% in nymphs, and 96.7% and 80.0% in adults, respectively, at the highest concentration (1 x 109 conidia/ml), 7 days after treatment. Overall results suggest that these two B. bassiana isolates have the potential to be used in the control of M. rosae.

Destekleyen Kurum

Akdeniz Üniversitesi

Proje Numarası

BAP FDK-2019-4859

Teşekkür

This study was financially supported by Akdeniz University Scientific Projects Coordination Unit (Project no.: BAP FDK-2019-4859) (Antalya, Türkiye). We would also like to thank TÜBİTAK BİDEB 2228-B Domestic Doctorate Scholarship Program.

Kaynakça

  • Abebe H., 2002. Potential of entomopathogenic fungi for the control of Macrotermes subhyalinus (Isoptera: Termitidae). Ph.D. thesis, Addis Ababa University, International Institute of Tropical Agriculture (IITA), Ethiopia, 161p.
  • Alghamdi A. S., 2018. Insecticidal effect of four plant essential oils against two aphid species under laboratory conditions. Journal of Applied Biology & Biotechnology 6 (2): 27-30.
  • Baki D., 2021. Antalya’da farklı bölgelerden izole edilen entomopatojen fungusların morfolojik ve moleküler tanısı, Aphis gossypii ve Myzus persicae’ye karşı etkinliklerinin belirlenmesi, Doktora Tezi, Akdeniz Üniversitesi, Antalya, 139 s.
  • Baki D., H. Ş. Tosun & F. Erler, 2021. "Indigenous entomopathogenic fungi as potential biological control agents of rose sawfly, Arge rosae L. (Hymenoptera: Argidae), Turkish Journal of Zoology, 45(7):517-525.
  • Budak E, Ş. Yigit, A.K. Aşkın, İ Akça, & İ. Saruhan, 2022. Bazı uçucu yağların Macrosiphum rosae (L.) (Hemiptera: Aphididae)'ya insektisidal etkilerinin belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 19 (1): 101-107.
  • Eidy M., H. Rafiee-Dastjerdi, F. Zargarzadeh, A. Golizadeh & V. Mahdavi, 2016. Pathogenicity of the entomopathogenic fungi Beauveria bassiana (Balsamo) and Verticillium lecanii (Zimmerman) against aphid Macrosiphum rosae, Linnaeus (Hemiptera: Aphididae) under laboratory conditions. Jordan Journal of Biological Sciences, 9. 25-28.
  • Fancelli M., A. B. Dias, I. J. Delalibera Cerqueira, S. de Jesus Souza, A. do Nascimento & S. Oliveira e Silva, 2013. Beauveria bassiana Strains for Biological Control of Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae) in Plantain. BioMed Research International, Volume 2013, Article ID 184756, 7s.
  • Gabarty A., H. M. Salem, M. A. Fouda, A. A. Abas & A. A. Ibrahim, 2014. Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon (Hufn.). Journal of Radiation Research and Applied Sciences, 7: 95-100.
  • İzgi Ü. & Ö. Güven, 2016. Kahramanmaraş Başkonuş ormanlık alanlarından izole edilen entomopatojen funguslar. Bitki Koruma Bülteni, 54 (3): 201-209.
  • Khosravi R, J.J. Sendi, A. Zibaee & A.M. Shokrgozar, 2015. Virulence of four Beauveria bassiana (Balsamo) (Asc., Hypocreales) isolates on rose sawfly, Arge rosae (Hymenoptera: Argidae) under laboratory condition. Journal of King Saudi University 27 (1): 49-53.
  • Özçelik H., 2013. General appearances of Turkish roses. Suleyman Demirel University Journal of Natural and Applied Science 17(1): 29-42.
  • Sáenz-de-Cabezón F.J., V. Irigaray, I. Marco–Mancebón & I. Pérez-Moreno, 2003. The entomopathogenic fungus Beauveria bassiana and compatibility with triflumeron: effects on the twospotted spider mite Tetranychus urticae, Biological Control, 26: 168–173.
  • Sastry K.S., B. Mandal, J. Hammond, S.W. Scott & R.W. Briddon, 2019. Rosa spp. (Rose). In: Encyclopedia of Plant Viruses and Viroids. Springer, New Delhi. doi: 10.1007/978-81-322-3912-3_797.
  • Sayed S.M., E.F. Ali, & S.S. Al-Otaibi, 2019. Efficacy of indigenous entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin, isolates against the rose aphid, Macrosiphum rosae L. (Hemiptera: Aphididae) in rose production. Egypt J Biol Pest Control 29: 19.
  • Timor A.N., 2011. World production oil rose and rose oil. e-Journal of New World Sciences Academy 6 (2): 4A0040.

Gül Yaprakbiti, Macrosiphum rosae L. (Hemiptera: Aphididae)’nin potansiyel biyolojik mücadele etmenleri olarak yerel entomopatojen funguslar

Yıl 2023, Cilt: 14 Sayı: 1, 76 - 83, 25.07.2023
https://doi.org/10.31019/tbmd.1278477

Öz

Gül yaprakbiti Macrosiphum rosae L. (Hemiptera: Aphididae), Türkiye'nin yanı sıra dünyanın birçok yerinde güllerin (Rosa spp.) (Rosaceae) önemli zararlılarından biridir. Bu çalışmada 5 yerel entomopatojen fungus izolatı [3 Beauveria bassiana izolatı (BbDm-1, BbKm-1, BbMp-1), 1 Isaria farinosa izolatı (IfGp-1) ve 1 Purpureocillium lilacinum izolatı (PlKa-1)] laboratuvar koşullarında M. rosae’nin nimflerine ve erginlerine karşı test edilmiştir. İzolatların 10 günlük PDA (patates dekstroz agar) ortamında kültüründen elde edilen 3 farklı konsantrasyondaki (1x107, 1x108 ve 1x109 konidia/ml) spor solüsyonları M. rosae’nin hem nimflerine hem de erginlerine karşı püskürtülerek test edilmiştir. Sonuçlar, ölüm oranının konsantrasyona bağımlı olduğunu ve izolatların etkinliğinin artan spor konsantrasyonuyla arttığını göstermiştir (P < 0.05). Test edilen 5 izolattan, 2 B. bassiana izolatı (BbDm-1 ve BbKm-1) en yüksek konsantrasyonda (1 x 109 konidia/ml) uygulamadan 7 gün sonra sırasıyla nimflerde %100 ve %83.3 ve yetişkinlerde %96.7 ve %80.0 ölümlere neden olmuştur. Elde edilen sonuçlar, bu iki B. bassiana izolatının M. rosae’nin kontrolünde kullanılma potansiyeline sahip olduğunu göstermektedir.

Proje Numarası

BAP FDK-2019-4859

Kaynakça

  • Abebe H., 2002. Potential of entomopathogenic fungi for the control of Macrotermes subhyalinus (Isoptera: Termitidae). Ph.D. thesis, Addis Ababa University, International Institute of Tropical Agriculture (IITA), Ethiopia, 161p.
  • Alghamdi A. S., 2018. Insecticidal effect of four plant essential oils against two aphid species under laboratory conditions. Journal of Applied Biology & Biotechnology 6 (2): 27-30.
  • Baki D., 2021. Antalya’da farklı bölgelerden izole edilen entomopatojen fungusların morfolojik ve moleküler tanısı, Aphis gossypii ve Myzus persicae’ye karşı etkinliklerinin belirlenmesi, Doktora Tezi, Akdeniz Üniversitesi, Antalya, 139 s.
  • Baki D., H. Ş. Tosun & F. Erler, 2021. "Indigenous entomopathogenic fungi as potential biological control agents of rose sawfly, Arge rosae L. (Hymenoptera: Argidae), Turkish Journal of Zoology, 45(7):517-525.
  • Budak E, Ş. Yigit, A.K. Aşkın, İ Akça, & İ. Saruhan, 2022. Bazı uçucu yağların Macrosiphum rosae (L.) (Hemiptera: Aphididae)'ya insektisidal etkilerinin belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 19 (1): 101-107.
  • Eidy M., H. Rafiee-Dastjerdi, F. Zargarzadeh, A. Golizadeh & V. Mahdavi, 2016. Pathogenicity of the entomopathogenic fungi Beauveria bassiana (Balsamo) and Verticillium lecanii (Zimmerman) against aphid Macrosiphum rosae, Linnaeus (Hemiptera: Aphididae) under laboratory conditions. Jordan Journal of Biological Sciences, 9. 25-28.
  • Fancelli M., A. B. Dias, I. J. Delalibera Cerqueira, S. de Jesus Souza, A. do Nascimento & S. Oliveira e Silva, 2013. Beauveria bassiana Strains for Biological Control of Cosmopolites sordidus (Germ.) (Coleoptera: Curculionidae) in Plantain. BioMed Research International, Volume 2013, Article ID 184756, 7s.
  • Gabarty A., H. M. Salem, M. A. Fouda, A. A. Abas & A. A. Ibrahim, 2014. Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon (Hufn.). Journal of Radiation Research and Applied Sciences, 7: 95-100.
  • İzgi Ü. & Ö. Güven, 2016. Kahramanmaraş Başkonuş ormanlık alanlarından izole edilen entomopatojen funguslar. Bitki Koruma Bülteni, 54 (3): 201-209.
  • Khosravi R, J.J. Sendi, A. Zibaee & A.M. Shokrgozar, 2015. Virulence of four Beauveria bassiana (Balsamo) (Asc., Hypocreales) isolates on rose sawfly, Arge rosae (Hymenoptera: Argidae) under laboratory condition. Journal of King Saudi University 27 (1): 49-53.
  • Özçelik H., 2013. General appearances of Turkish roses. Suleyman Demirel University Journal of Natural and Applied Science 17(1): 29-42.
  • Sáenz-de-Cabezón F.J., V. Irigaray, I. Marco–Mancebón & I. Pérez-Moreno, 2003. The entomopathogenic fungus Beauveria bassiana and compatibility with triflumeron: effects on the twospotted spider mite Tetranychus urticae, Biological Control, 26: 168–173.
  • Sastry K.S., B. Mandal, J. Hammond, S.W. Scott & R.W. Briddon, 2019. Rosa spp. (Rose). In: Encyclopedia of Plant Viruses and Viroids. Springer, New Delhi. doi: 10.1007/978-81-322-3912-3_797.
  • Sayed S.M., E.F. Ali, & S.S. Al-Otaibi, 2019. Efficacy of indigenous entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin, isolates against the rose aphid, Macrosiphum rosae L. (Hemiptera: Aphididae) in rose production. Egypt J Biol Pest Control 29: 19.
  • Timor A.N., 2011. World production oil rose and rose oil. e-Journal of New World Sciences Academy 6 (2): 4A0040.
Toplam 15 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği, Tarımda Entomoloji
Bölüm Makaleler
Yazarlar

Derya Baki 0000-0003-1285-169X

Hilal Şule Tosun 0000-0001-8360-2610

Fedai Erler 0000-0002-7216-9871

Proje Numarası BAP FDK-2019-4859
Erken Görünüm Tarihi 3 Temmuz 2023
Yayımlanma Tarihi 25 Temmuz 2023
Gönderilme Tarihi 7 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 14 Sayı: 1

Kaynak Göster

APA Baki, D., Tosun, H. Ş., & Erler, F. (2023). Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae). Türkiye Biyolojik Mücadele Dergisi, 14(1), 76-83. https://doi.org/10.31019/tbmd.1278477
AMA Baki D, Tosun HŞ, Erler F. Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae). Türk. biyo. müc. derg. Temmuz 2023;14(1):76-83. doi:10.31019/tbmd.1278477
Chicago Baki, Derya, Hilal Şule Tosun, ve Fedai Erler. “Indigenous Turkish Entomopathogenic Fungi As Potential Biological Control Agents of the Rose Aphid, Macrosiphum Rosae L. (Hemiptera: Aphididae)”. Türkiye Biyolojik Mücadele Dergisi 14, sy. 1 (Temmuz 2023): 76-83. https://doi.org/10.31019/tbmd.1278477.
EndNote Baki D, Tosun HŞ, Erler F (01 Temmuz 2023) Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae). Türkiye Biyolojik Mücadele Dergisi 14 1 76–83.
IEEE D. Baki, H. Ş. Tosun, ve F. Erler, “Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae)”, Türk. biyo. müc. derg, c. 14, sy. 1, ss. 76–83, 2023, doi: 10.31019/tbmd.1278477.
ISNAD Baki, Derya vd. “Indigenous Turkish Entomopathogenic Fungi As Potential Biological Control Agents of the Rose Aphid, Macrosiphum Rosae L. (Hemiptera: Aphididae)”. Türkiye Biyolojik Mücadele Dergisi 14/1 (Temmuz 2023), 76-83. https://doi.org/10.31019/tbmd.1278477.
JAMA Baki D, Tosun HŞ, Erler F. Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae). Türk. biyo. müc. derg. 2023;14:76–83.
MLA Baki, Derya vd. “Indigenous Turkish Entomopathogenic Fungi As Potential Biological Control Agents of the Rose Aphid, Macrosiphum Rosae L. (Hemiptera: Aphididae)”. Türkiye Biyolojik Mücadele Dergisi, c. 14, sy. 1, 2023, ss. 76-83, doi:10.31019/tbmd.1278477.
Vancouver Baki D, Tosun HŞ, Erler F. Indigenous Turkish entomopathogenic fungi as potential biological control agents of the Rose Aphid, Macrosiphum rosae L. (Hemiptera: Aphididae). Türk. biyo. müc. derg. 2023;14(1):76-83.