Antioxidant activity, amino acid composition and trace element levels of Verbascum lasianthum Boiss. ex Bentham from Erzincan, Türkiye

Abstract

The aim of this study is to investigate trace elements and amino acids in the root and stem of Verbascum lasianthum Boiss. ex Bentham (VL) from Erzincan and to understand the role of these compounds in adaptation to environmental stress conditions. In the study, ICP-MS and LC-MS/MS methods are used for analyses. In addition, antioxidant activities of methanol:water extracts (70:30, v:v) of these parts were investigated. The results showed that VL in Erzincan accumulates elements such as sodium and silicium to adapt to arid and stressful environmental conditions and supports these processes by synthesizing amino acids such as L-proline, L-asparagine and L-glutamine. The high amount of iron indicates that VL can be a potential iron source in the treatment of anemia. However, aluminum accumulation in plants is extremely risky due to its possible neurotoxic effect in humans and plant toxicity. Although these amino acids and trace elements contribute to the antioxidant potential of the plant, VL showed a low antioxidant activity when compared to VL species in other regions. In conclusion, this study emphasizes the importance of comprehensively analyzing phytochemical profiles such as trace elements, amino acids and phenolic compounds to evaluate the efficacy and safety of medicinal plants.

Keywords

• Verbascum lasianthum
• trace elements
• amino acids
• antioxidant activity
• medicinal plant

Türkiye, Erzincan'dan Verbascum lasianthum Boiss. ex Bentham’ın antioksidan aktivitesi, amino asit bileşimi ve eser element seviyeleri

Abstract

Bu çalışmanın amacı, Erzincan'dan Verbascum lasianthum Boiss. ex Bentham.'ın (VL) kök ve gövdesindeki eser elementleri ve amino asitleri araştırmak ve bu bileşenlerin çevresel stres koşullarına uyum sağlamadaki rolünü anlamaktır. Çalışmada, analizler için ICP-MS ve LC-MS/MS yöntemleri kullanılmıştır. Ayrıca, bu kısımların metanol:su (70:30, v:v) ekstraklarının antioksidan aktiviteleri araştırılmıştır. Sonuçlar, Erzincan'daki VL'nin kurak ve stresli çevre koşullarına uyum sağlamak için sodyum ve silisyum gibi elementleri biriktirdiğini ve L-prolin, L-asparagin ve L-glutamin gibi amino asitleri sentezleyerek bu süreçleri desteklediğini göstermiştir. Yüksek demir miktarı, VL'nin anemi tedavisinde potansiyel bir demir kaynağı olabileceğini göstermektedir. Ancak alüminyumun bitkilerde birikmesi, insanlarda nörotoksik etki yaratması ve bitki toksisitesi nedeniyle son derece risklidir. Bu amino asitler ve eser elementler bu bitkinin antioksidan potansiyeline katkıda bulunsa da, diğer bölgelerdeki VL türleriyle karşılaştırıldığında düşük bir antioksidan aktivite gösterdi. Sonuç olarak, bu çalışma tıbbi bitkilerin tıbbi etkinliğini ve güvenliğini değerlendirmek için eser elementler ve amino asitler gibi fitokimyasal profillerin kapsamlı bir şekilde analiz edilmesinin önemini vurgulamaktadır.

Keywords

• Verbascum lasianthum
• eser elementler
• amino asitler
• antioksidan aktivite
• tıbbi bitki

References

1. Thomson GE. Further consideration of Asian medicinal plants in treating common chronic diseases in the West. Journal of Medicinal Plants Research. 2010;4:125–30.
2. Sixty-second World Health Assembly: Resolutions and decisions, annexes. 2009.
3. Sattar SA, Reddy BS, Rao VK, Pradeep A, Raju GN, Ramanarayana K, et al. Estimation of trace elements in some anti-epileptic medicinal plants by PIXE. Journal of Radioanalytical and Nuclear Chemistry. 2012;294:337–41.
4. Silva PSC, Francisconi LS, Gonçalves RD. Evaluation of major and trace elements in medicinal plants. Journal of the Brazilian Chemical Society. 2016;27:2273–89.
5. Ranđelović SS, Kostić DA, Zarubica AR, Mitić SS, Mitić MN. The correlation of metal content in medicinal plants and their water extracts. Hemijska Industrija (Chemical Industry). 2013;67:585–91.
6. Ebrahim AM, Eltayeb MH, Khalid H, Mohamed H, Abdalla W, Grill P, et al. Study on selected trace elements and heavy metals in some popular medicinal plants from Sudan. Journal of Natural Medicines. 2012;66:671–9.
7. Rengel Z, Cakmak I, White P. Marschner’s mineral nutrition of plants. Acad Press. 2022.
8. Baqir H, Zeboon N, Al-Behadili A. The role and importance of amino acids within plants: A review. Plant Archives. 2019;19:1402–10.

9. Karamian R, Ghasemlou F. Total phenolic content, antioxidant and antibacterial activities of three Verbascum species from Iran. Journal of Medicinal plants and By-Products. 2013;2:43–51.
10. Şimşek S, Akşit H, Aydın A, Köksal E. Ferruginoside D: A novel phenylethanoid from Verbascum leiocarpum. Chemistry & Biodiversity. 2023;20:e202301200.
11. Süntar I, Tatli II, Küpeli Akkol E, Keleş H, Kahraman Ç, Akdemir Z. An ethnopharmacological study on Verbascum species: From conventional wound healing use to scientific verification. Journal of Ethnopharmacology. 2010;132:408–13.
12. Kupeli E, Tatli II, Akdemir ZS, Yesilada E. Bioassay-guided isolation of anti-inflammatory and antinociceptive glycoterpenoids from the flowers of Verbascum lasianthum Boiss. ex Bentham. Journal of Ethnopharmacology. 2007;110:444–50.
13. Hazman O, Aksoy L, Buyukben A, Kara R, Kargioglu M, Kumral ZB, et al. Evaluation of antioxidant, cytotoxic, antibacterial effects and mineral levels of Verbascum lasianthum Boiss. ex Bentham. Anais da Academia Brasileira de Ciências. 2021;93:e20210865.
14. Atila A, Alay H, Yaman ME, Akman TC, Cadirci E, Bayrak B, et al. The serum amino acid profile in COVID-19. Amino Acids. 2021;53:1569–88.
15. Gözcü S, Akşit Z, Aydın A, Yılmaz MA, Şimşek S. Comprehensive phenolic profiling and biological evaluation of Centaurea glastifolia L. (Asteraceae). Natural Product Research. 2024;1–12.
16. Varol T, Osma E, Şimşek S, Elveren M. Antioxidant activities of plant species growing in different habitats (serpentine, gypsum and limestone). Frontiers in Life Sciences and Related Technologies. 2023;4:150–6.
17. Gözcü S, Akşit Z, Şimşek S, Kandemir A, Aydin A, Yilmaz MA, et al. LC-MS /MS characterization and biological activities of Morina persica L. (Caprifoliaceae). Journal of Research in Pharmacy. 2024; 28:961–73.
18. Akman TÇ, Şimşek S, Akşit Z, Aydın A, Yılmaz MA. Exploring the potential of Psephellus huber-Morathi (Wagenitz) Wagenitz: A comprehensive UHPLC-MS/MS analysis of phytochemical composition and evaluation of antioxidant, antimicrobial, and antiproliferative activities. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2024;27:782–92.
19. Akman TC, Yazici M, Atila A, Mertoglu C. Analysis of Isotretinoin-Induced alterations in the levels of plasma trace elements: Investigation of the relationship between magnesium, phosphorus, potassium, zinc, and Treatment-Related side effects. Biological Trace Element Research. 2024;202:4858–4868.
20. Palani K, Balasubramanian K, Kalaivani R. Study on aluminium contamination in mettur soil and its subsequent uptake by medicinal plants. Oriental Journal of Chemistry. 2018;34:3129.
21. Kaur H, Kaur H, Kaur H, Srivastava S. The beneficial roles of trace and ultratrace elements in plants. Plant Growth Regulation. 2022;100:219–36.
22. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology. 2014;7:60–72.
23. Swain S, Rautray TR. Estimation of trace elements, antioxidants, and antibacterial agents of regularly consumed indian medicinal plants. Biological Trace Element Research. 2021;199:1185–93.
24. Alotaibi MO, Saleh AM, Sobrinho RL, Sheteiwy MS, El‐sawah AM, Mohammed AE, et al. Arbuscular mycorrhizae mitigate aluminum toxicity and regulate proline metabolism in plants grown in acidic soil. Journal of Fungi. 2021;7:531.
25. Alvarez M, Savouré A, Szabados L. Proline metabolism as regulatory hub. Trends in Plant Science. 2022;27:39–55.
26. Buchanan B, Gruissem W, Jones R. Biochemistry and molecular biology of plants. John Wiley & Sons, Ltd; 2015.
27. Lardos M, Marmagne A, Bonadé Bottino N, Caris Q, Béal B, Chardon F, et al. Discovery of the biostimulant effect of asparagine and glutamine on plant growth in Arabidopsis thaliana. Frontiers in Plant Science. 2023;14:1281495.