Antioxidant activity, amino acid composition and trace element levels of Verbascum lasianthum Boiss. ex Bentham from Erzincan, Türkiye

Öz

The aim of this study is to investigate trace elements and amino acids in the root and stem of Verbascum lasianthum Boiss. ex Bentham (VL) from Erzincan and to understand the role of these compounds in adaptation to environmental stress conditions. In the study, ICP-MS and LC-MS/MS methods are used for analyses. In addition, antioxidant activities of methanol:water extracts (70:30, v:v) of these parts were investigated. The results showed that VL in Erzincan accumulates elements such as sodium and silicium to adapt to arid and stressful environmental conditions and supports these processes by synthesizing amino acids such as L-proline, L-asparagine and L-glutamine. The high amount of iron indicates that VL can be a potential iron source in the treatment of anemia. However, aluminum accumulation in plants is extremely risky due to its possible neurotoxic effect in humans and plant toxicity. Although these amino acids and trace elements contribute to the antioxidant potential of the plant, VL showed a low antioxidant activity when compared to VL species in other regions. In conclusion, this study emphasizes the importance of comprehensively analyzing phytochemical profiles such as trace elements, amino acids and phenolic compounds to evaluate the efficacy and safety of medicinal plants.

Anahtar Kelimeler

• Verbascum lasianthum
• trace elements
• amino acids
• antioxidant activity
• medicinal plant

Türkiye, Erzincan'dan Verbascum lasianthum Boiss. ex Bentham’ın antioksidan aktivitesi, amino asit bileşimi ve eser element seviyeleri

Öz

Bu çalışmanın amacı, Erzincan'dan Verbascum lasianthum Boiss. ex Bentham.'ın (VL) kök ve gövdesindeki eser elementleri ve amino asitleri araştırmak ve bu bileşenlerin çevresel stres koşullarına uyum sağlamadaki rolünü anlamaktır. Çalışmada, analizler için ICP-MS ve LC-MS/MS yöntemleri kullanılmıştır. Ayrıca, bu kısımların metanol:su (70:30, v:v) ekstraklarının antioksidan aktiviteleri araştırılmıştır. Sonuçlar, Erzincan'daki VL'nin kurak ve stresli çevre koşullarına uyum sağlamak için sodyum ve silisyum gibi elementleri biriktirdiğini ve L-prolin, L-asparagin ve L-glutamin gibi amino asitleri sentezleyerek bu süreçleri desteklediğini göstermiştir. Yüksek demir miktarı, VL'nin anemi tedavisinde potansiyel bir demir kaynağı olabileceğini göstermektedir. Ancak alüminyumun bitkilerde birikmesi, insanlarda nörotoksik etki yaratması ve bitki toksisitesi nedeniyle son derece risklidir. Bu amino asitler ve eser elementler bu bitkinin antioksidan potansiyeline katkıda bulunsa da, diğer bölgelerdeki VL türleriyle karşılaştırıldığında düşük bir antioksidan aktivite gösterdi. Sonuç olarak, bu çalışma tıbbi bitkilerin tıbbi etkinliğini ve güvenliğini değerlendirmek için eser elementler ve amino asitler gibi fitokimyasal profillerin kapsamlı bir şekilde analiz edilmesinin önemini vurgulamaktadır.

Anahtar Kelimeler

• Verbascum lasianthum
• eser elementler
• amino asitler
• antioksidan aktivite
• tıbbi bitki

Kaynakça

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