Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

H. Turan Akkoyun; A. Şükrü Bengü; Mahire Bayramoğlu Akkoyun; Aykut Ulucan; Mehmet Sait İzgi; Ömer Şahin; Suat Ekin; Şule Melek

doi:10.46810/tdfd.819741

Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi

Abstract

Bu çalışmada, sıçanlarda civa II klorür'ün meydana getireceği beyin hasarının engellenmesinde borik asit (BA)'in koruyucu etkileri araştırıldı. Deneyde 200-300 gr ağırlığında, 24 adet yetişkin, Wistar albino cinsi erkek rat kullanıldı. Ratlar, Grup I (Kontrol,n:8):İzotonik serum uygulandı (i.p), Grup II (civa II klorür (HgCl2),n:8):Oral yol ile (0.01 g/kg), Grup III (HgCl2+BA,n:8): Oral yol ile HgCl2 (0.01 g/kg/gün) + BA (3.25mg/kg/gün) (i.p) konsantrasyonda uygulaması yapıldı. Tüm gruplardaki ratlar 10. günün sonunda sakrifiye edilerek beyin dokuları alındı. Biyokimyasal parametrelerden SOD, CAT ve GSH-Px antioksidan enzim aktiviteleri ölçüldü. Ayrıca histopatolojik olarak değerlendirildi. SOD enzim aktivitesi değerlendirildiğinde; Hg uygulanan grupta kontrole oranla azaldığı (p˂0.001) görüldü. BA, uygulanan grupta ise aktivitenin HgCl2 grubu ile kıyaslandığında arttığı (p˂0.001) tespit edildi. CAT enzim aktivitesi değerlendirildiğinde; kontrole oranla HgCl2 ve HgCl2+BA uygulanan gruplarda aktivitede azalma (p˂0.001) olduğu görüldü. HgCl2 uygulanan grupla karşılaştırıldığında BA uygulanan grupta enzim aktivitesinde artış belirlendi. GSH-Px enzim aktivitesi değerlendirildiğinde ise; kontrol grubu ile kıyaslandığında HgCl2 (p˂0.001) ve HgCl2+BA (p˂0.05) uygulanan gruplarda aktivitenin azaldığı görüldü. Ayrıca HgCl2 uygulanan grup ile karşılaştırıldığında BA uygulanan grupta artış olduğu görüldü (p˂0.001). SOD, CAT ve GSH-Px gibi antioksidan enzim aktivitelerinin anlamlı olarak değiştiği ve beyin dokusu histopatolojik incelemede BA'nın koruyucu etkisi olabileceği düşünülmektedir.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

H. Turan Akkoyun ^*
0000-0002-4547-8003
Türkiye

A. Şükrü Bengü
Türkiye

Mahire Bayramoğlu Akkoyun
0000-0001-5150-5402
Türkiye

Aykut Ulucan
0000-0001-8844-8237
Türkiye

Mehmet Sait İzgi
0000-0003-3685-3219
Türkiye

Ömer Şahin
0000-0003-4575-3762
Türkiye

Suat Ekin
0000-0002-6502-5028
Türkiye

Şule Melek
0000-0002-0677-722X
Türkiye

Publication Date

December 30, 2020

Submission Date

November 2, 2020

Acceptance Date

December 14, 2020

Published in Issue

Year 2020 Volume: 9 Number: 2

DOI

https://doi.org/10.46810/tdfd.819741

IZ

https://izlik.org/JA72SL85ML

Cite

RIS / Bibtex

APA

Akkoyun, H. T., Bengü, A. Ş., Bayramoğlu Akkoyun, M., Ulucan, A., İzgi, M. S., Şahin, Ö., Ekin, S., & Melek, Ş. (2020). Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. Turkish Journal of Nature and Science, 9(2), 127-133. https://doi.org/10.46810/tdfd.819741

AMA

1.Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, et al. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TJNS. 2020;9(2):127-133. doi:10.46810/tdfd.819741

Chicago

Akkoyun, H. Turan, A. Şükrü Bengü, Mahire Bayramoğlu Akkoyun, et al. 2020. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Turkish Journal of Nature and Science 9 (2): 127-33. https://doi.org/10.46810/tdfd.819741.

EndNote

Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, Ulucan A, İzgi MS, Şahin Ö, Ekin S, Melek Ş (December 1, 2020) Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. Turkish Journal of Nature and Science 9 2 127–133.

IEEE

[1]H. T. Akkoyun et al., “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”, TJNS, vol. 9, no. 2, pp. 127–133, Dec. 2020, doi: 10.46810/tdfd.819741.

ISNAD

Akkoyun, H. Turan - Bengü, A. Şükrü - Bayramoğlu Akkoyun, Mahire - Ulucan, Aykut - İzgi, Mehmet Sait - Şahin, Ömer - Ekin, Suat - Melek, Şule. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Turkish Journal of Nature and Science 9/2 (December 1, 2020): 127-133. https://doi.org/10.46810/tdfd.819741.

JAMA

1.Akkoyun HT, Bengü AŞ, Bayramoğlu Akkoyun M, Ulucan A, İzgi MS, Şahin Ö, Ekin S, Melek Ş. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TJNS. 2020;9:127–133.

MLA

Akkoyun, H. Turan, et al. “Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi”. Turkish Journal of Nature and Science, vol. 9, no. 2, Dec. 2020, pp. 127-33, doi:10.46810/tdfd.819741.

Vancouver

1.H. Turan Akkoyun, A. Şükrü Bengü, Mahire Bayramoğlu Akkoyun, Aykut Ulucan, Mehmet Sait İzgi, Ömer Şahin, Suat Ekin, Şule Melek. Civa II Klorürün Sıçan Beyin Dokusunda Oluşturduğu Toksisiteye Karşı Borik Asidin Koruyucu Etkisinin İncelenmesi. TJNS. 2020 Dec. 1;9(2):127-33. doi:10.46810/tdfd.819741