Pinus nigra subsp. pallasiana ve Pinus brutia ağaçlarında in vitro ve in situ koşullarda ölçülen fotosentetik gaz değişim parametrelerinin karşılaştırılması

Abstract

Bu çalışma, iki çam türünde gaz değişim parametrelerini belirlemede kullanılabilecek in situ ve in vitro metotların sonuçlarının karşılaştırılması amacıyla yapılmıştır. Çalışma, Türkiye'nin Batı Akdeniz Bölgesi’nde yer alan yarı kurak iklim tipine sahip Kemer/Burdur orman alanındaki Pinus nigra Arn. subsp. pallasiana (Lamb). Holmboe ve Pinus brutia Ten. karışık plantasyon sahasında gerçekleştirilmiştir. Birinci yöntem olarak her iki türde gaz değişim parametreleri (net fotosentez hızı- Anet, stoma iletkenliği-gs ve terleme oranı-E) kurulan bir iskele yardımıyla doğrudan ağaç üzerinde ölçülmüştür (in situ koşulları temsilen). İkinci yöntemde, ölçüm yapılan ibrelerin bulunduğu dallar yaklaşık 50 cm’den kesilip suya daldırılarak aynı ibreler üzerinde gaz değişim parametreleri belirlenmiştir (in vitro koşulları temsilen). Çalışma 2022 yılının Haziran-Ekim ayları arasında gerçekleştirilmiştir. In situ ve in vitro fotosentez ölçüm yöntemlerinin sonuçlarını karşılaştırmak için Student t testi uygulanmıştır. Örnekleme günlerinde, iki türde de gaz değişim parametreleri bakımından uygulanan metotların sonuçları arasında istatistiksel olarak anlamlı bir farklılık belirlenmemiştir. Çalışma sonuçları, boylu ağaçların dallarına erişimin mümkün olmadığı arazi koşullarında P. nigra ve P. brutia türlerinde in situ fotosentez ölçümleri yerine in vitro fotosentez ölçümlerinin tercih edilebileceğini göstermiştir.

Keywords

• Anadolu karaçamı
• Dal kesme
• Gaz değişimi
• Yerinde ölçüm
• Akdeniz Ormanları
• Kızılçam

A comparison of photosynthetic gas exchange parameters measured under in situ and in vitro conditions in Pinus nigra subsp. pallasiana and Pinus brutia trees

Abstract

This study was conducted to compare the results of in situ and in vitro methods that can be used for measuring gas exchange parameters in two pine species. The study was carried out in a mixed Pinus nigra Arn. subsp. pallasiana (Lamb). Holmboe and Pinus brutia Ten. plantation in Kemer/Burdur, which has a semi-arid climate type located in the Western Mediterranean Region of Türkiye. In the first method, gas exchange parameters (net photosynthetic rate-Anet, stomatal conductance-gs and transpiration rate-E) were measured directly on the tree using a mobile scaffold to reach to the tree canopy (in situ conditions). In the second method, branches were cut at approximately 50 cm and gas exchange parameters were determined in the same needles after the branch was immediately submerged in water (in vitro conditions). Measurements were taken between June and October 2022. Student’s t-test was conducted to compare the results of in situ and in vitro photosynthesis measurement methods. No statistically significant differences were found between the results of the compared methods in terms of gas exchange parameters for both species. The results of the study showed that in vitro measurements of photosynthesis can be preferred to in situ measurements of photosynthesis in P. nigra and P. brutia under field conditions where access to the canopy of tall trees is not possible.

Keywords

• Anatolian black pine
• Branch cutting
• Gas exchange
• In situ measurement
• Mediterranean forest
• Turkish red pine

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