Research Article
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Assessing the Acute Toxicity of Chlorantraniliprole and Flubendiamide in Bombus terrestris Workers of Different Ages

Year 2021, Volume 3, Issue 2, 57 - 61, 22.12.2021

Abstract

Bombus terrestris colonies are used for pollination in greenhouse tomato cultivation. Different-ages workers in these colonies involved in foraging activities in line with the necessities of the colony face the risk of exposure to chemicals used. Therefore, exposure of forager workers to chemicals directly and other individuals in the colony indirectly may affect the pollination performance of colonies. For this reason, knowing the effect of chemicals used in pest control on bumblebees is also important in terms of effectively benefiting from colonies for pollination. This study was carried out to investigate the effects of the recommended doses of insecticide active ingredients with Chlorantraniliprole and Flubendiamide (12 g/100 L water and 15 g/100 L water, respectively), which are highly preferred against Tuta absoluta pest in tomato cultivation, on B. terrestris worker bees of different ages. Insecticides were applied to workers of 1 day, 3 days, 7 days, and 10 days of age by spraying method and the total of 60 microcolonies were prepared, 15 each in each age group. Microcolonies formed by workers exposed to insecticides were checked on the 1st, 3rd, 5th, and 7th days and the number of dying worker bees was recorded. When the results were examined, the total workers mortality rate in all age and experiment groups remained below 25%. According to the International Organization for Biological Control (IOBC) toxicity scale, plant protection products with the active ingredient Chlorantraniliprole and Flubendiamide used in the study were found to be non-toxic to B. terrestris worker bees of different ages.

References

  • Abbes, K., Harbi, A., & Chermiti, B. (2012). The tomato leafminer Tuta absoluta (Meyrick) in Tunisia: current status and management strategies. EPPO Bulletin, 42(2), 226-233. https://www.doi.org/10.1111/epp.2559
  • Aguiar, A. P., Deans, A. R., Engel, M. S., Forshage, M., Huber, J. T., Jennings, J. T., Johnson, N. F., Lelej A. S., Longıno J. T., Lohrmann, V., Miko, I., Ohl M., Rasmussen, C., Taeger A., & Yu, D. S. K. (2013). Order Hymenoptera. In: Zhang, Z.-Q. (Ed.) Animal Biodiversity: An Outline of Higher-level Classification and Survey of Taxonomic Richness (Addenda 2013). Zootaxa, 3703(1), 51-62.
  • Ahmad, M., Bodlah, I., Mehmood, K., Sheikh, U. A. A., & Aziz, M. A. (2015). Pollination and foraging potential of European bumblebee, Bombus terrestris (Hymenoptera: Apidae) on tomato crop under greenhouse system. Pakistan Journal of Zoology, 47(5), 1279-1285.
  • Brittain, C., & Potts, S. G. (2011). The potential impacts of insecticides on the life-history traits of bees and the consequences for pollination. Basic and Applied Ecology, 12(4), 321-331. https://www.doi.org/10.1016/j.baae.2010.12.004.
  • Canbay, A., Alaserhat, İ., & Tohma, Ö. (2014). Erzincan ve Iğdır illeri domates alanlarında zararlı Tuta absoluta (Meyrick) (Lep.: Gelechiidae) ve predatörlerinin popülasyon takibi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 45(2), 79-97.
  • Dinter A., Brugger K. E., Frost N. M., & Woodward MD (2009) Chlorantraniliprole (Rynaxypyr): a novel DuPont insecticide with low toxicity and low risk for honey bees (Apis mellifera) and bumblebees (Bombus terrestris) providing excellent tools for uses in integrated pest management. Julius-KuhnArchiv, 423, 84–96.
  • Erler, F., Can, M., Erdogan, M., Ates, A. O., & Pradier, T. (2010). New record of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on greenhouse-grown tomato in southwestern Turkey (Antalya). Journal of Entomological Science, 45(4), 392-393. https://www.doi.org/10.18474/0749-8004-45.4.392.
  • Gill, R. J., & Raine, N. E. (2014). Chronic impairment of bumblebee natural foraging behaviour induced by sublethal pesticide exposure. Functional Ecology, 28(6), 1459-1471. https://doi.org/10.1111/1365-2435.12292 Gösterit, A., & Gürel, F. (2010). Bombus arıları ve bitkisel üretim açısından önemleri. Arıcılık Araştırma Dergisi, 4, 9-12.
  • Gösterit, A., & Gürel, F. (2018). The role of commercially produced bumblebees in good agricultural practices. Scientific Papers, Series D., Animal Science, 5, 201– 204.
  • Gradish, A. E., Scott‐Dupree, C. D., Shipp, L., Harris, C. R., & Ferguson, G. (2009). Effect of reduced risk pesticides for use in greenhouse vegetable production on Bombus impatiens (Hymenoptera: Apidae). Pest Management Science: formerly Pesticide Science, 66(2), 142-146. https://www.doi.org/10.1002/ps.1846.
  • Gradish, A. E., Scott-Dupree, C. D., Frewin, A. J., & Cutler, G. C. (2012). Lethal and sublethal effects of some insecticides recommended for wild blueberry on the pollinator Bombus impatiens. The Canadian Entomologist, 144(3), 478-486.
  • Gradish, A. E., Van Der Steen, J., Scott-Dupree, C. D., Cabrera, A. R., Cutler, G. C., Goulson, D., Klein O., Lehmann D. M., Lückmann J., O’Neill B., Raine N. E., Sharma B., & Thompson, H. (2019). Comparison of pesticide exposure in honey bees (Hymenoptera: Apidae) and bumble bees (Hymenoptera: Apidae): implications for risk assessments. Environmental Entomology, 48(1), 12-21.
  • Kılıç, T. (2010). First record of Tuta absoluta in Turkey. Phytoparasitica, 38(3), 243-244. https://www.doi.org/10.1007/s12600-010-0095-7.
  • Kirubhadharsini, B. L., Gunasekaran, K., & Krishnamoorthy, S. V. (2019). Assessment of safety of flubendiamide 20 WG against non-target organisms. Journal of Entomology and Zoology Studies 2019; 7(6), 612-616.
  • Klinger, E. G., Camp, A. A., Strange, J. P., Cox-Foster, D., & Lehmann, D. M. (2019). Bombus (Hymenoptera: Apidae) microcolonies as a tool for biological understanding and pesticide risk assessment. Environmental Entomology, 48(6), 1249-1259. https://www.doi.org/10.1093/ee/nvz117.
  • Kwon, Y. J., & Saeed, S. (2003). Effect of temperature on the foraging activity of Bombus terrestris L. (Hymenoptera: Apidae) on greenhouse hot pepper (Capsicum annuum L.). Applied Entomology and Zoology, 38(3), 275-280.
  • Lahm, G. P., Selby, T. P., Freudenberger, J. H., Stevenson, T. M., Myers, B. J., Seburyamo, G., Smith, B. K., Flexner, L., Clark C. E., & Cordova, D. (2005). Insecticidal anthranilic diamides: a new class of potent ryanodine receptor activators. Bioorganic & Medicinal Chemistry Letters, 15(22), 4898-4906. https://www.doi.org/10.1016/j.bmcl.2005.08.034.
  • Larson, J. L., Redmond, C. T., Potter, D. A. (2013). Assessing insecticide hazard to bumble bees foraging on flowering weeds in treated lawns. PLoS One, 8(6), e66375. https://www.doi.org/10.1371/journal.pone.0066375.
  • Mamay, M. & Yanik, E. (2012). Şanlıurfa’da domates alanlarında Domates Güvesi [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)]’nin ergin populasyon gelişiminin belirlenmesi. Türkiye Entomoloji Bülteni, 2(3), 189-198.
  • Mommaerts, V. & Smagghe, G. (2011). Side-effects of pesticides on the pollinator Bombus: an overview. In-Tech., 507-552.
  • Portakaldalı, M., Öztemiz, S., Kütük, H., Büyüköztürk, H., & Ateş, A. (2013). Doğu Akdeniz ve Güneydoğu Anadolu Bölgeleri’nde Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nın yayılış durumu. Türkiye Entomoloji Bülteni, 3(3), 133-139.
  • Roditakis, E., Skarmoutsou, C., & Staurakaki, M. (2013). Toxicity of insecticides to populations of tomato borer Tuta absoluta (Meyrick) from Greece. Pest Management Science, 69(7), 834-840. https://www.doi.org/10.1002/ps.3442.
  • Roditakis, E., Vasakis, E., Grispou, M., Stavrakaki, M., Nauen, R., Gravouil, M., & Bassi, A. (2015). First report of Tuta absoluta resistance to diamide insecticides. Journal of Pest Science, 88(1), 9-16. https://www.doi.org/10.1007/s10340-015-0643-5.
  • Sterk, G., Heuts, F., Merck, N., & Bock, J. (2002). Sensitivity of non-target arthropods and beneficial fungal species to chemical and biological plant protection products: results of laboratory and semi-field trials. 1st international symposium on biological control of arthropods, 306-313.
  • Tobback, J., Mommaerts, V., Vandersmissen, H. P., Smagghe, G., & Huybrechts, R. (2011). Age‐and task‐dependent foraging gene expression in the bumblebee Bombus terrestris. Archives of Insect Biochemistry and Physiology, 76(1), 30-42. https://www.doi.org/10.1002/arch.20401.
  • Ünlü, L. (2011). Domates güvesi, Tuta absoluta (Meyrick)'nın Konya ilinde örtüaltında yetiştirilen domateslerdeki varlığı ve populasyon değişimi. Selçuk Tarım ve Gıda Bilimleri Dergisi, 25(4), 27-29.
  • Velthuis, H. H. & van Doorn, A. (2006). A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie, 37(4), 421-451. https://www.doi.org/10.1051/apido:2006019.
  • Williams, P. H. (1998). An annotated checklist of bumble bees with an analysis of patterns of description (Hymenoptera: Apidae, Bombini). Bulletin-Natural History Museum Entomology Series, 67, 79-152.
  • Whitehorn, P. R., O’Connor, S., Wackers, F. L., & Goulson, D. (2012). Neonicotinoid pesticide reduces bumble bee colony growth and queen production. Science, 336(6079), 351-352. https://www.doi.org/10.1126/science.1215025.

Farklı Yaşlardaki Bombus terrestris L. İşçi Arılarında Chlorantraniliprole ve Flubendiamide’nin Akut Toksisitesinin Değerlendirilmesi

Year 2021, Volume 3, Issue 2, 57 - 61, 22.12.2021

Abstract

Örtü altı domates yetiştiriciliğinde tozlaşmanın sağlanması amacıyla kullanılan Bombus terrestris L. kolonilerinde, koloninin ihtiyacı doğrultusunda tarlacılık faaliyetinde yer alan farklı yaştaki işçi arılar kullanılan kimyasallara maruz kalması kolonilerin tozlaştırma performansını etkileyebilmektedir. Bu nedenle zararlılar ile mücadelede kullanılan kimyasalların farklı yaştaki bombus arıları üzerine etkisinin bilinmesi kolonilerden tozlaşma amacıyla etkin bir şekilde yararlanılması açısından da önemlidir. Bu çalışma domates yetiştiriciliğinde Tuta absoluta (Meyrick) zararlısına karşı yoğun olarak tercih edilen Chlorantraniliprole (Altacor) ve Flubendiamide (Takumi) etken maddeli insektisitlerin tavsiye edilen dozlarının (sırasıyla; 12 g/100 L su ve 15 g/100 L su) farklı yaşlardaki B. terrestris işçi arıları üzerine etkisinin araştırılması amacıyla gerçekleştirilmiştir. İnsektisitler 1, 3, 7 ve 10 günlük yaştaki işçi arılara püskürtme yöntemi ile uygulanmıştır ve her bir yaş grubunda 15’er adet olmak üzere toplam 60 mikro koloni hazırlanmıştır. İnsektisitlere maruz kalan işçi arılar ile oluşturulan mikro koloniler 1., 3., 5. ve 7. günlerde kontrol edilmiş ve ölen işçi arı sayıları kaydedilmiştir. Elde edilen sonuçlar incelendiğinde tüm yaş ve ilaç gruplarında toplam işçi arı ölüm oranı %25’in altında kalmış ve Uluslararası Biyolojik Mücadele Organizasyonu (IOBC) toksisite skalasına göre, çalışmada kullanılan Chlorantraniliprole ve Flubendiamide etken maddeli bitki koruma ürünlerinin farklı yaşlardaki B. terrestris işçi arıları için zehirsiz olduğu belirlenmiştir.

References

  • Abbes, K., Harbi, A., & Chermiti, B. (2012). The tomato leafminer Tuta absoluta (Meyrick) in Tunisia: current status and management strategies. EPPO Bulletin, 42(2), 226-233. https://www.doi.org/10.1111/epp.2559
  • Aguiar, A. P., Deans, A. R., Engel, M. S., Forshage, M., Huber, J. T., Jennings, J. T., Johnson, N. F., Lelej A. S., Longıno J. T., Lohrmann, V., Miko, I., Ohl M., Rasmussen, C., Taeger A., & Yu, D. S. K. (2013). Order Hymenoptera. In: Zhang, Z.-Q. (Ed.) Animal Biodiversity: An Outline of Higher-level Classification and Survey of Taxonomic Richness (Addenda 2013). Zootaxa, 3703(1), 51-62.
  • Ahmad, M., Bodlah, I., Mehmood, K., Sheikh, U. A. A., & Aziz, M. A. (2015). Pollination and foraging potential of European bumblebee, Bombus terrestris (Hymenoptera: Apidae) on tomato crop under greenhouse system. Pakistan Journal of Zoology, 47(5), 1279-1285.
  • Brittain, C., & Potts, S. G. (2011). The potential impacts of insecticides on the life-history traits of bees and the consequences for pollination. Basic and Applied Ecology, 12(4), 321-331. https://www.doi.org/10.1016/j.baae.2010.12.004.
  • Canbay, A., Alaserhat, İ., & Tohma, Ö. (2014). Erzincan ve Iğdır illeri domates alanlarında zararlı Tuta absoluta (Meyrick) (Lep.: Gelechiidae) ve predatörlerinin popülasyon takibi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 45(2), 79-97.
  • Dinter A., Brugger K. E., Frost N. M., & Woodward MD (2009) Chlorantraniliprole (Rynaxypyr): a novel DuPont insecticide with low toxicity and low risk for honey bees (Apis mellifera) and bumblebees (Bombus terrestris) providing excellent tools for uses in integrated pest management. Julius-KuhnArchiv, 423, 84–96.
  • Erler, F., Can, M., Erdogan, M., Ates, A. O., & Pradier, T. (2010). New record of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on greenhouse-grown tomato in southwestern Turkey (Antalya). Journal of Entomological Science, 45(4), 392-393. https://www.doi.org/10.18474/0749-8004-45.4.392.
  • Gill, R. J., & Raine, N. E. (2014). Chronic impairment of bumblebee natural foraging behaviour induced by sublethal pesticide exposure. Functional Ecology, 28(6), 1459-1471. https://doi.org/10.1111/1365-2435.12292 Gösterit, A., & Gürel, F. (2010). Bombus arıları ve bitkisel üretim açısından önemleri. Arıcılık Araştırma Dergisi, 4, 9-12.
  • Gösterit, A., & Gürel, F. (2018). The role of commercially produced bumblebees in good agricultural practices. Scientific Papers, Series D., Animal Science, 5, 201– 204.
  • Gradish, A. E., Scott‐Dupree, C. D., Shipp, L., Harris, C. R., & Ferguson, G. (2009). Effect of reduced risk pesticides for use in greenhouse vegetable production on Bombus impatiens (Hymenoptera: Apidae). Pest Management Science: formerly Pesticide Science, 66(2), 142-146. https://www.doi.org/10.1002/ps.1846.
  • Gradish, A. E., Scott-Dupree, C. D., Frewin, A. J., & Cutler, G. C. (2012). Lethal and sublethal effects of some insecticides recommended for wild blueberry on the pollinator Bombus impatiens. The Canadian Entomologist, 144(3), 478-486.
  • Gradish, A. E., Van Der Steen, J., Scott-Dupree, C. D., Cabrera, A. R., Cutler, G. C., Goulson, D., Klein O., Lehmann D. M., Lückmann J., O’Neill B., Raine N. E., Sharma B., & Thompson, H. (2019). Comparison of pesticide exposure in honey bees (Hymenoptera: Apidae) and bumble bees (Hymenoptera: Apidae): implications for risk assessments. Environmental Entomology, 48(1), 12-21.
  • Kılıç, T. (2010). First record of Tuta absoluta in Turkey. Phytoparasitica, 38(3), 243-244. https://www.doi.org/10.1007/s12600-010-0095-7.
  • Kirubhadharsini, B. L., Gunasekaran, K., & Krishnamoorthy, S. V. (2019). Assessment of safety of flubendiamide 20 WG against non-target organisms. Journal of Entomology and Zoology Studies 2019; 7(6), 612-616.
  • Klinger, E. G., Camp, A. A., Strange, J. P., Cox-Foster, D., & Lehmann, D. M. (2019). Bombus (Hymenoptera: Apidae) microcolonies as a tool for biological understanding and pesticide risk assessment. Environmental Entomology, 48(6), 1249-1259. https://www.doi.org/10.1093/ee/nvz117.
  • Kwon, Y. J., & Saeed, S. (2003). Effect of temperature on the foraging activity of Bombus terrestris L. (Hymenoptera: Apidae) on greenhouse hot pepper (Capsicum annuum L.). Applied Entomology and Zoology, 38(3), 275-280.
  • Lahm, G. P., Selby, T. P., Freudenberger, J. H., Stevenson, T. M., Myers, B. J., Seburyamo, G., Smith, B. K., Flexner, L., Clark C. E., & Cordova, D. (2005). Insecticidal anthranilic diamides: a new class of potent ryanodine receptor activators. Bioorganic & Medicinal Chemistry Letters, 15(22), 4898-4906. https://www.doi.org/10.1016/j.bmcl.2005.08.034.
  • Larson, J. L., Redmond, C. T., Potter, D. A. (2013). Assessing insecticide hazard to bumble bees foraging on flowering weeds in treated lawns. PLoS One, 8(6), e66375. https://www.doi.org/10.1371/journal.pone.0066375.
  • Mamay, M. & Yanik, E. (2012). Şanlıurfa’da domates alanlarında Domates Güvesi [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)]’nin ergin populasyon gelişiminin belirlenmesi. Türkiye Entomoloji Bülteni, 2(3), 189-198.
  • Mommaerts, V. & Smagghe, G. (2011). Side-effects of pesticides on the pollinator Bombus: an overview. In-Tech., 507-552.
  • Portakaldalı, M., Öztemiz, S., Kütük, H., Büyüköztürk, H., & Ateş, A. (2013). Doğu Akdeniz ve Güneydoğu Anadolu Bölgeleri’nde Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nın yayılış durumu. Türkiye Entomoloji Bülteni, 3(3), 133-139.
  • Roditakis, E., Skarmoutsou, C., & Staurakaki, M. (2013). Toxicity of insecticides to populations of tomato borer Tuta absoluta (Meyrick) from Greece. Pest Management Science, 69(7), 834-840. https://www.doi.org/10.1002/ps.3442.
  • Roditakis, E., Vasakis, E., Grispou, M., Stavrakaki, M., Nauen, R., Gravouil, M., & Bassi, A. (2015). First report of Tuta absoluta resistance to diamide insecticides. Journal of Pest Science, 88(1), 9-16. https://www.doi.org/10.1007/s10340-015-0643-5.
  • Sterk, G., Heuts, F., Merck, N., & Bock, J. (2002). Sensitivity of non-target arthropods and beneficial fungal species to chemical and biological plant protection products: results of laboratory and semi-field trials. 1st international symposium on biological control of arthropods, 306-313.
  • Tobback, J., Mommaerts, V., Vandersmissen, H. P., Smagghe, G., & Huybrechts, R. (2011). Age‐and task‐dependent foraging gene expression in the bumblebee Bombus terrestris. Archives of Insect Biochemistry and Physiology, 76(1), 30-42. https://www.doi.org/10.1002/arch.20401.
  • Ünlü, L. (2011). Domates güvesi, Tuta absoluta (Meyrick)'nın Konya ilinde örtüaltında yetiştirilen domateslerdeki varlığı ve populasyon değişimi. Selçuk Tarım ve Gıda Bilimleri Dergisi, 25(4), 27-29.
  • Velthuis, H. H. & van Doorn, A. (2006). A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie, 37(4), 421-451. https://www.doi.org/10.1051/apido:2006019.
  • Williams, P. H. (1998). An annotated checklist of bumble bees with an analysis of patterns of description (Hymenoptera: Apidae, Bombini). Bulletin-Natural History Museum Entomology Series, 67, 79-152.
  • Whitehorn, P. R., O’Connor, S., Wackers, F. L., & Goulson, D. (2012). Neonicotinoid pesticide reduces bumble bee colony growth and queen production. Science, 336(6079), 351-352. https://www.doi.org/10.1126/science.1215025.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Research Articles
Authors

İsmail Yaşhan BULUŞ (Primary Author)
ısparta uygulamalı bilimler üniversitesi
0000-0003-4418-588X
Türkiye


Asiye UZUN
Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü
0000-0002-4822-4762
Türkiye


Ozan DEMİRÖZER
Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü
0000-0001-7240-8898
Türkiye


Ayhan GÖSTERİT
Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü
0000-0001-9686-7992
Türkiye

Publication Date December 22, 2021
Published in Issue Year 2021, Volume 3, Issue 2

Cite

APA Buluş, İ. Y. , Uzun, A. , Demirözer, O. & Gösterit, A. (2021). Farklı Yaşlardaki Bombus terrestris L. İşçi Arılarında Chlorantraniliprole ve Flubendiamide’nin Akut Toksisitesinin Değerlendirilmesi . Türk Bilim ve Mühendislik Dergisi , 3 (2) , 57-61 . Retrieved from https://dergipark.org.tr/en/pub/tjse/issue/66406/968931