Karpuz Mozaik Virüsü (WMV - 2)’nün Doğal Konukçusu Sicyos

Abstract

Bu çalışma dünyanın önemli istilacı türleri arasında yer alan ve ülkemizde de Karadeniz Bölgesi’nde yayılmış olan Sicyos angulatus’un konukçuluk ettiği viral etmenleri belirlemek amacıyla yapılmıştır. Virüslerin tanılanması Double Anti body Sandwich ELİSA (DAS ELİSA) testi ile Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Watermelon Mosaic Virus (WMV-2), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya RingspotVirus(PRSV), PotatoVirus Y(PVY) antiserumları kullanılarak yapılmıştır. Yapılan testlerde Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya Ring spot Virus(PRSV), Potato Virus Y (PVY) virüslerine rastlanmamış olup sadece WMV-2 tespit edilmiştir.Türkiye’de WMV-2’nin S. angulatus’da doğal olarak enfeksiyon oluşturduğu ilk kez bu çalışma ile tespit edilmiştir

Keywords

• Sicyos angulatus L.,karpuz mozaik virüsü, konukçu

Sicyos angulatus : A Natural Host of Watermelon Mosaic Virus (WMV-2)

Abstract

This study was aimed at detection of virusin bur cucumber (Sicyos angulatus L.) collected from Black Sea region. Double antibody sandwich enzyme-linked immuno sorbent assay (DAS-ELISA) was used to test the 11 leaf samples for the presence of following viruses; Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Watermelon Mosaic Virus (WMV-2), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya Ring spot Virus (PRSV), Potato Virus Y(PVY). Among tested viruses, only WMV-2 was identified from the collected leaves. To our knowledge, this is the first report of natural occurrence of WMV-2 in S. angulatus in Turkey

Keywords

• Sicyos angulatus L.,watermelon mosaic virus, host

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