Karpuz Mozaik Virüsü (WMV - 2)’nün Doğal Konukçusu Sicyos

Öz

Bu çalışma dünyanın önemli istilacı türleri arasında yer alan ve ülkemizde de Karadeniz Bölgesi’nde yayılmış olan Sicyos angulatus’un konukçuluk ettiği viral etmenleri belirlemek amacıyla yapılmıştır. Virüslerin tanılanması Double Anti body Sandwich ELİSA (DAS ELİSA) testi ile Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Watermelon Mosaic Virus (WMV-2), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya RingspotVirus(PRSV), PotatoVirus Y(PVY) antiserumları kullanılarak yapılmıştır. Yapılan testlerde Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya Ring spot Virus(PRSV), Potato Virus Y (PVY) virüslerine rastlanmamış olup sadece WMV-2 tespit edilmiştir.Türkiye’de WMV-2’nin S. angulatus’da doğal olarak enfeksiyon oluşturduğu ilk kez bu çalışma ile tespit edilmiştir

Anahtar Kelimeler

• Sicyos angulatus L.,karpuz mozaik virüsü, konukçu

Sicyos angulatus : A Natural Host of Watermelon Mosaic Virus (WMV-2)

Öz

This study was aimed at detection of virusin bur cucumber (Sicyos angulatus L.) collected from Black Sea region. Double antibody sandwich enzyme-linked immuno sorbent assay (DAS-ELISA) was used to test the 11 leaf samples for the presence of following viruses; Cucumber Mosaic Virus (CMV), Tobacco Mosaic Virus (TMV), Tomato Mosaic Virus (ToMV), Watermelon Mosaic Virus (WMV-2), Zucchini Yellow Mosaic Virus (ZYMV), Squash Mosaic Virus (SqMV), Papaya Ring spot Virus (PRSV), Potato Virus Y(PVY). Among tested viruses, only WMV-2 was identified from the collected leaves. To our knowledge, this is the first report of natural occurrence of WMV-2 in S. angulatus in Turkey

Anahtar Kelimeler

• Sicyos angulatus L.,watermelon mosaic virus, host

Kaynakça

1. Anonim, (2013). Uzungöl Özel Çevre Koruma Bölgesi Yönetim Planı 2013-2017. T.C. Çevre ve Şehircilik Bakanlığı Tabiat Varlıkları Koruma Genel Müdürlüğü. http://www.csb.gov.tr/db/ tabiat/editordosya /uzungol_yonetim_plani.
2. Clark, M. F. and Adams, A.M. 1977. Characteristics of the 8 16 2 microplatemethod of enzyme-linked immuno sorbent assay for the detection of plantviruses. J. Gen. Virol. 34:475-483.
3. Delmiglio, C. And Pearson M.N. (2006). Effect sandincidence of cucumber mosaic virus, watermelon mosaic virus and zucchini yellow mosaic virus in New Zealand’s only native cucurbit, Sicyos australis. Australasian Plant Pathology, 35:29–35.
4. Desbiez, C.,Costa, C.Wipf‒Scheibel, C.GirardM.andLecoq, H. (2007). Serologicaland molecular variability of watermelon mosaic virus (genusPotyvirus). Arch. Virol., 152: 775‒781.
5. EPPO, (2010). Datasheet on invasive alien plants. Sicyos angulatus. Bulletin OEPP/EPPO Bulletin 40(3): 401-406.
6. Esbenshade, W.R.,Curran, W.S., Roth, G.W., Hartwig, N.L., and Orzolek, M.D. (2001). Effect of establishment date and crop competition on bur cucumber fecundity. Weed Science 49:524-527.
7. Hong, S., Park, J., Cho, S. and Shin, H. (2014). First report of Cercospora Leaf spot of burcucumber caused by Cercospora cirullina in Korea. Journal of Phytopathology, 162:338-341.
8. Kurokawa,S., Kobayashi, H.andSenda, T. (2009). Genetic diversity of Sicyos angulatus in cetraland North-eastern Japan by inter-simple sequence repeat analysis. Weed Res., 49:365-372.

9. Messersmith, D.T.,Curran, W.S., Harrwig, N.L., Orzolek, M.D. and Roth, G.W. (1999). Evaluation of several herbicides for bur cucumber (Sicyos angulatus) control in corn (Zeamays).Weed Technology 13: 520-524.
10. Messersmith, D.T.,Curran, W.S., Roth, G.W., Harrwig, N.L. and Orzolek, M.D. (2000). Tillage and herbicides affect bur cucumber management in corn. Agronomy Journal 92:181-185.
11. Önen,H., Özaslan, C., Günal, H., Akyol, N. ve Caldıran, U. (2013). Expansion status of two invasive vines. Bur-cucumber and Mile-a-Minute in Turkey. 4th Esenias Workshop. International Workshop on IAS in Agricultural and NonAgricultural Areas in ESENİAS Region. 16-17 December 2013 Çanakkale, Turkey.
12. Önen, H., Özaslan, C.ve Tad, S. (2015). Sicyos angulatus.Türkiye İstilacı Bitkiler Kataloğu (edit: Önen, H.) 458-472.
13. Sebastian, P.,Schaefer,H., Telford, I.R.H. and Renner S.S. (2010).Cucumber (Cucumis sativus) and melon (C. melo) have numerous wild relatives in Asia and Australia, and the sister species of melon is from Australia. Proceedings of the National Academy of Sciences of the United States of America 107(14) 269–273.
14. Shimizu, N. (1999). Thelevel of damage by the foreign weed Sicyos angulatus. Weed Science,49(1): 99-105.
15. Terzioğlu, T.ve Anşin, R. (1999). Türkiye’nin ekzotik bitkilerine bir katkı. Sicyos angulatusL.Tr. J. Agriculture and Forestry.23:359-362.
16. Zitter, T.A. (2001). A Checklist of Major Weeds and Crops as Natural Hosts for Plant Viruses in the Northeast. http://vegetablemdonline/ ppath.cornell.edu/ Tables/ WeedHost Table.html.