Investigating the Effect of Testosterone Supplement on Heart and Muscle Damage in Rats Applied with Swimming Exercise

Year 2019, Volume: 21 Issue: 1, 170 - 174, 30.04.2019

Ömer Kulaksız Sefa Lök

https://doi.org/10.15314/tsed.552893

Abstract

Anabolic
Androgenic Steroids which are a synthetic derivative of testosterone are known
to be used by some sportsmen in order to improve performance. The present study
was conducted to investigate the effect of testosterone supplement on heart and
muscle damage in rats applied with swimming exercise. The study was conducted
on 30 day-old 27 (Wistar, male) rats that were supplied from “Selçuk University
Experimental Medicine Research and Application Center”. The rats were divided
into 4 groups. The rats in control groups (n:6) were fed during the study
without doing any application. The rats in testosteronegroup (n:7) were
supplied with a 10 mg/kg dose of testosterone 5 days a week for 3 weeks. The
rats in exercise group (n:7) did freestyle swimming exercise for 30 minutes a
day for 5 days a weekfor 3 weeks. The rats in testosterone + exercise group
(n:7) did freestyle swimming exercise for 30 minutes and were administered with
10 mg/kg dose of testosterone supplement for 5 days a week for 3 weeks. At the
end of the trial, necessary biochemical parameters were measured in serums
obtained by drawing blood from tails of the rats. While levels of Serum, LDH,
CK-MB, and CKwere studied by using an autoanalyzer, Troponin I level was
analyzed by using ELISA kit. SPSS 15.0 packaged software was usedin statistical
evaluation of the data and the results were given as mean±SE. Since the data
were convenient with the normal distribution, ANOVA test was applied for
betweengroups comparison and Duncan test was used to identify from which group
the difference was resulted. When between-groups values of troponin I, ck-MB,
and LDH, which are indicators of heart damage, and enzyme CK, which is an
indicator of muscle damage,for control, exercise, testosterone, and
testosterone+exercise groups were compared, a statistically significant
difference was determined between control, exercise, testosterone, and
testosterone+exercise groups (p<0.05). As a result of statistically analysis
performed on all groups, the difference was found to be caused by testosterone
and testosterone+exercise groups. Because testosterone production is naturally
increased in the body during the exercise, swimming exercise and testosterone
administration led to significant occurrence of troponin I, ck-MB, LDH, which
are heart damage indicators in rats, and CK value, which is an indicator of
muscle damage, in the blood.

Keywords: Testosterone,
Heart, Muscle Damage, Swimming Exercise

Keywords

Testosterone, Heart, Muscle Damage

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