Helichrysum armenium species used in alternative medicine (Altınotu) phenolic content and antioxidant properties

Year 2024, , 490 - 496, 30.04.2024

Fatma Ergün , Melike Yeğin

https://doi.org/10.30910/turkjans.1355478

Abstract

Radical accumulation is among the causes of chronic diseases in living organisms. These radicals formed as a result of metabolic activities must be neutralized and removed from the environment in order to reduce or eliminate their harmful effects on the organism. This is possible thanks to natural antioxidants. The knowledge of this situation has led to an increased interest in natural antioxidant sources and many studies have been conducted. Helichrysum armenium subsp. armenium is a species of the Asteraceae family with medicinal and aromatic properties with antioxidant potential. It has a wide distribution worldwide and is used in traditional medicine for various folkloric purposes. The phenolic and flavonoid content of the extract of this plant species used in our study was 87.60±0.10 mg GAE g-1 and 38.84±1.36 mg QE g-1, respectively. IC50 value of the plant extract was 183.07±4.92 µg ml-1, Fe+3 reducing power was 2.36±0.20 µg TE ml-1, Cu+2 reducing power was 40.94±4.62 µg TE ml-1, DPPH radical scavenging activity was 24.05+1.13 µg TE ml-1. In addition, vitamin C value was determined as 17.2 mg 100 g-1. These results reveal the richness of the biochemical content of Helichrysum armenium subsp. armenium taxon. Considering the results, it is concluded that the intense interest in other sub-taxa of the genus Helichrysum should also be shown to this species. Further studies are also needed to determine the health impacts of this species.

Keywords

Antioxidant, Phytochemicals, Helichrysum armenium subsp. armenium, Altınotu, Ölmezotu

Alternatif Tıpta Kullanılan Helichrysum armenium Türünün (Altınotu) Fenolik İçerik ve Antioksidan Özellikleri

Abstract

Canlılardaki kronik hastalıkların oluşma sebepleri arasında radikal akümülasyonu gelmektedir. Metabolik faaliyetler sonucu oluşan bu radikallerin organizma üzerindeki zararlı etkilerinin azaltılması veya yok edilmesi için nötrleştirilip ortamdan uzaklaştırılması gerekir. Buda doğal antioksidanlar sayesiyle mümkündür. Bu durumun bilinmesi doğal antioksidan kaynaklarına ilginin artmasına ve birçok çalışmanın yapılmasına neden olmuştur. Helichrysum armenium subsp. armenium, Asteraceae familyasına ait antioksidan potansiyele sahip tıbbi ve aromatik özellikleri olan bir türdür. Dünya çapında geniş bir dağılıma sahip olan bu bitki, geleneksel tıpta çeşitli folklorik amaçlar için kullanılmaktadır. Çalışmamızda kullanılan bu bitki türü ekstraktının fenolik madde miktarının 87,60±0,10 mg GAE g-1 ve flavonoid madde miktarının 38,84±1,36 mg KE g-1 olduğu belirlenmiştir. Bitki ekstraktının, IC50 değerinin 183,07±4,92 µg mL-1, Fe+3 indirgeme gücünün 2,36±0,20 µg TE/ml, Cu+2 indirgeme gücünün 40,94±4,62 µg TE mL-1, DPPH radikal giderme aktivitesinin ise 24,05+1,13 µg TE mL-1 olduğu tespit edilmiştir. Ayrıca C vitamini değeri 17,2 mg 100 g-1 olarak belirlendi. Bu sonuçlar, Helichrysum armenium subsp. armenium taksonunun biyokimyasal içeriğinin zenginliğini ortaya koymaktadır. Sonuçlar dikkate alındığında daha çok Helichrysum cinsinin diğer alt taksonlarına olan yoğun ilginin, bu türe karşıda gösterilmesi gerekliliği kanaati oluşmuştur. Ayrıca bu türün sağlık üzerindeki etkilerini belirlemek amacıyla yeni çalışmaların yapılmasına ihtiyaç vardır.

Keywords

Antioksidan, Fitokimyasallar, Helichrysum armenium subsp. armenium, Altınotu, Ölmezotu

References

• Acet, T., Özcan, K. 2023. Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye. Karadeniz Fen Bilimleri Dergisi. 13(2): 680-689.
• Akaberi, M., Sahebkar, A., Azizi, N. ve Emami, S. A. 2019. Everlasting flowers: Phytochemistry and pharmacology of the genus Helichrysum. Industrial Crops and Products, 138.
• Akinyede, K. A., Cupido, C. N., Hughes, G. D., Oguntibeju, O. O. ve Ekpo, O. E. 2021. Medicinal properties and In vitro biological activities of selected Helichrysum species from South Africa: A review. Plants, 10(8): 1566.
• Albayrak, S., Aksoy, A., Sagdic, O., Hamzaoglu, E. 2010a. Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food chemistry. 119(1): 114-122.
• Albayrak, S., Aksoy, A., Sagdic, O., Budak, Ü. 2010b. Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology. 34(4): 463-473.
• Apak R., Güçlü K., Özyürek M., Karademir S.E. 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. Journal of Agricultural and Food Chemistry. 52(26): 7970-7981.
• Babotă, M., Mocan, A., Vlase, L., Crișan, O., Ielciu, I., Gheldiu, A. M., Vodnar D. C., Çrişan G., Păltinean, R. 2018. Phytochemical analysis, antioxidant and antimicrobial activities of Helichrysum arenarium (L.) Moench. and Antennaria dioica (L.) Gaertn. flowers. Molecules. 23(2): 409.
• Bandonienė, D., Murkovic, M. 2002. On-line HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from apples (Malus domestica L.). Journal of Agricultural and Food Chemistry. 50(9): 2482-2487.
• Blois M.S. 1958. Antioxidant determinations by the use of a stable free radical, Nature. 181: 1199-1200. Boubakeur, H., Rebbas, K., Belhattab, R. 2017. Antioxidant and antibacterial activities of the extracts of Helichrysum stoechas (L.) Moench. Phytothérapie, 1-11.
• Carmona, F. ve Pereira, A. M. S. 2013. Herbal medicines: old and new concepts, truths and misunderstandings. Revista Brasileira de Farmacognosia, 23(2): 379-385.
• Czinner, E., Lemberkovics, É., Bihátsi-Karsai, E., Vitányi, G. ve Lelik, L. 2000. Composition of the essential oil from the inflorescence of Helichrysum arenarium (L.) Moench. Journal of Essential Oil Research, 12(6): 728-730.
• Demirci, S. ve Özhatay, N. 2012. An ethnobotanical study in Kahramanmaraş (Turkey); wild plants used for medicinal purpose in Andirin, Kahramanmaraş. Turk J Pharm Sci, 9(1): 75-92.
• Ergün, F. 2021a. Cotoneaster Transcaucasicus Pojark. Determination of bioactive component amounts and antioxidant activities in fruit extracts. Turkish Journal of Agriculture-Food Science and Technology. 9(7): 1258-1263.
• Ergün, F. 2021b. Kırşehir’de Yetiştirilen Cemele Biberinin Biyoaktif Bileşenlerinin ve Antioksidan Kapasitesinin Belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi. 8(3): 693-701.
• Ergün, F. 2022a. Determination of bioactive chemicals and antioxidant capacity in different plant parts of Coriander (Coriandrum Sativum L.). JAPS: Journal of Animal & Plant Sciences. 32(2): 532-537.
• Ergün, F. 2022b. The effects of drying methods on total phenolic and flavonoid substances and antioxidant capacity of Redstem Filaree (Erodium Cicutarium). Applied Ecology and Environmental Research. 20(1): 499-509.
• Erlund, I., Koli, R., Alfthan, G., Marniemi, J., Puukka, P., Mustonen, P., Mattila, P., Jula, A. 2008. Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol. The Am. J. of Clin. Nutrition. 87(2): 323-331.
• Eroğlu, H. E. 2018. Türkiye Helichrysum taksonlarının Türkçe ve diğer dillerdeki isimleri. Avrasya Terim Dergisi, 6(1): 26-34.
• Eroğlu, H. E., Hamzaoğlu, E., Aksoy, A., Budak, Ü. Ve Albayrak, S. 2010. Helichrysum arenarium'un insan lenfosit kültürlerinde sitogenetik etkileri. Türk Biyoloji Dergisi , 34 (3): 253-256.
• Faqi, A. S. 2016. A comprehensive guide to toxicology in nonclinical drug development. Academic Press. Güngör, Ö. 2022. Kozmetikte esansiyel yağlar. Premium e-Journal of Social Science. 6(24): 409-413.
• Güzel, M., Akpınar, Ö. 2019. Valorisation of fruit by-products: production characterization of pectins from fruit peels. Food Bioprod Process. 115: 126-133.
• Iriti, M., Varoni, E. M. Ve Vitalini, S. 2010. Melatonin in traditional Mediterranean diets. Journal of pineal research, 49(2): 101-105.
• Mükemre, M., Behçet, L. ve Çakılcıoğlu, U. 2015. Ethnobotanical study on medicinal plants in villages of Çatak (Van-Turkey). Journal of ethnopharmacology, 166: 361-374.
• Oyaizu, M. 1986. Study on products of Browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315.
• Perrini, R., Morone-Fortunato, I., Lorusso, E. ve Avato, P. 2009. Glands, essential oils and in vitro establishment of Helichrysum italicum (Roth) G. Don ssp. microphyllum (Willd.) Nyman. Industrial crops and products, 29(2-3): 395-403.
• Reidel, R. V. B., Cioni, P. L., Ruffoni, B., Cervelli, C. ve Pistelli, L. 2017. Aroma profile and essential oil composition of Helichrysum species. Natural Product Communications, 12(9).
• Rigano, D., Formisano, C., Pagano, E., Senatore, F., Piacente, S., Masullo, M. ve Borrelli, F. 2014. A new acetophenone derivative from flowers of Helichrysum italicum (Roth) Don ssp. italicum. Fitoterapia, 99:198-203.
• Sardesai, V. M. 2002. Herbal medicines: poisons or potions?. Journal of Laboratory and Clinical Medicine, 139(6): 343-348.
• Slinkard, K., Singleton, V.L. 1977. Total phenol analyses: automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49-55.
• Stankov, S., Fidan, H., Petkova, N., Stoyanova, A., Dincheva, I., Doğan, H., Coşge, Ş.B., Uskutoğlu, T., Baş, H., Yılmaz, G. 2020. Phytochemical composition of Helichrysum arenarium (L.) Moench essential oil (aerial parts) from Turkey. Ukrainian Food Journal. 9(3): 503-512.
• Thangapazham, R.L., Sharma, A., Maheshwari, R.K. 2006. Multiple molecular targets in cancer chemoprevention by curcumin. The AAPS Journal. 8(3): 443.
• Velioglu, Y., Mazza, G., Gao, L., Oomah, B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of agricultural and food chemistry. 46(10): 4113-4117.
• Viegas, D. A., Palmeira-de-Oliveira, A., Salgueiro, L., Martinez-de-Oliveira, J. ve Palmeira-de-Oliveira, R. 2014. Helichrysum italicum: From traditional use to scientific data. Journal of ethnopharmacology, 151(1): 54-65.
• Zengin, G., Cvetanović, A., Gašić, U., Tešić, Ž., Stupar, A., Bulut, G., Sinan, K.I., Uysal, Ş., Picot-allain, M.C.N., Mahomoodally, M.F. 2020. A comparative exploration of the phytochemical profiles and bio-pharmaceutical potential of Helichrysum stoechas subsp. barrelieri extracts obtained via five extraction techniques. Process Biochemistry. 91: 113-125.