Micropropagation and Acclimatization of Large Cardamom (Amomum subulatum Roxb.)

Year 2018, , 231 - 235, 26.07.2018

Krishna Poudel Hari Kumar Prasaı Jiban Shrestha

https://doi.org/10.30910/turkjans.448318

Abstract

In vitro clonal propagation
and acclimatization of the tissue culture plant of large
cardamom has been established at Agriculture Research Station Pakhribas,
Dhankuta, Nepal. The rhizome buds were collected from the screen house and then
cultured in Murashige and Skoog (MS) medium enriched with 9 different
concentrations of BAP (6-benzylaminopurine) and IBA (indole-3-butyric acid)
namely 0.5 mg/L (BAP), 1.0 mg/L (BAP), 1.5 mg/L (BAP), 2.0 mg/L (BAP),  1 mg/L (IBA), 0.5 mg/L (BAP)+ 1 mg/L (IBA),
1.0 mg/L (BAP)+ 1 mg/L (IBA), 1.5 mg/L (BAP)+ 1 mg/L (IBA) and 2.0 mg/L (BAP)+
1 mg/L (IBA). This study showed that the explants grown in MS medium
supplemented with 1.0 mg / L BAP + 1.0 mg / L IBA showed the maximum root
induction rate. Buds produced roots in the same medium. The roots planted in
the screen house were transplanted for the hardening process. Then these
hardened plants were transferred to the netted nursery for further
multiplication process. This protocol developed could be used for developing
the superior quality plants of large cardamom.

Keywords

Acclimatization, explants, large cardamom, micropropagation.

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