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Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nda Flavobacterium psychrophilum’a Karşı Formalinle Öldürülmüş Bir Aşının Etkisi

Year 2014, Volume: 1 Issue: 2, 215 - 218, 26.07.2014

Abstract

Vaccine development for rainbow trout fry syndrome (RTFS) has been based primarily on whole-cell bacterins. In this study, protective effects of candidate strain to develop a vaccine in future studies against to Flavobacterium psychrophilum were investigated. Alteration in the relative percentage of survival rate (RPS) of rainbow trout exposed to 1×108 CFU/mL formalin-killed cells for 30, 60 and 90 min. Upon challenge with a strain the RPS of respectively, 21.74%, 34.78% and 43.48% was recorded. The results suggested that a weak protection had obtained with this strain, therefore; the strain was not suitable for use in vaccine development.

References

  • Amend, D.F., 1981. Potency testing of fish vaccines. Developments in Biological Standardization. 49: 447–454.
  • Borg, A.F., 1960. Studies on myxobacteria associated with diseases in salmonid fishes. J Wildl. Dis. 8: 1–85.
  • Dalsgaard, I. and Madsen, L., 2000. Bacterial Pathogens in Rainbow Trout Oncorhynchus mykiss (Walbaum), Reared At Danish Freshwater Farms. J. Fish Dis. 23: 199–209. Elsayed, E.E., Eissa, A.E. and Faisal, M., 2006. Isolation of Flavobacterium psychrophilum from sea lamprey, Petromyzon marinus L., with skin lesions in Lake Ontario. J. Fish Dis. 29: 629–632.
  • Holt, R.A., 1987. Cytophaga psychrophila, the causative agent of bacterial coldwater disease in salmonid fish. Doctoral dissertation. Oregon State University. Corvallis, Oregon.
  • Holt, R.A., Rohovec, J.S. and Fryer, J.L., 1993. Bacterial coldwater disease. In: Inglis V, Roberts R.J., Bromage N.R. (eds) Bacterial diseases of fish. Blackwell Scientific Publications. Oxford. 3–23 p.
  • Lida, Y. and Mizokami, A., 1996. Outbreaks of coldwater disease in wild ayu and pale chub. Fish Pathol. 31: 157–164
  • Kent, M.L., Groff, J.M., Morrison, J.K., Yasutake, W.T. and Holt, R.A., 1989. Spiral swimming behavior due to cranial and vertebral lesions associated with Cytophaga psychrophila infections in salmonid fishes. Diseases of Aquatic Organisms. 6: 11–16.
  • Klesius, P.H., Shoemaker, C.A. and Evans, J.J., 1999. Efficacy of a killed Streptococcus iniae vaccine in tilapia (Oreochromis niloticus). Bull. Eur. Assoc. Fish Pathol. 19(1): 39-41.
  • Kondo, M., Kawai, K., Okabe, M., Nakano, N. and Oshima, S.I., 2003. Efficacy of oral vaccine against bacterial coldwater disease in ayu Plecoglossus altivelis. Diseases of Aquatic Organisms. 55: 261–264
  • LaFrentz, B.R., LaPatra, S.E., Jones, G.R., Congleton, J.L., Sun, B. and. Cain, K.D., 2002. Characterization of serum and mucosal antibody responses and relative percent survival in rainbow trout, Oncorhynchus mykiss (Walbaum), following immunization and challenge with Flavobacterium psychrophilum. J. Fish Dis. 25: 703–713.
  • Lorenzen, E., 1994. Studies on Flexibacter pychrophilus in relation to rainbow trout fry syndrome (RTFS). National Veterinary Laboratory and Royal Veterinary and Agricultural University. Arhus. 1-25 p.
  • Lorenzen, E. and Olesen, N.J., 19 Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome II: serological studies. Diseases of Aquatic Organisms. 31: 209–220. Lorenzen, E., Brudeseth, B.E., Wiklund, T. and Lorenzen, N., 2010. Immersion exposure of rainbow trout (Oncorhynchus mykiss) fry to wildtype Flavobacterium psychrophilum induces no mortality, but protects against later intraperitoneal challenge. Fish and Shellfish Immunology. 28: 440-444
  • Madetoja, J., Lönnström, L.G., Björkblom, C., Uluköy, G., Bylund, G., Syvertsen, C., Gravningen, K., Norderhus, E.A. and Wiklund, T., 2006. Efficacy of injection vaccines against Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. 29: 9–20.
  • Obach, K., and Laurencin, F.B., 1991. Vaccination of rainbow trout Oncorhynchus mykiss against the visceral form of coldwater disease. Dis. Aquat. Org. 12: 13–15.
  • Ostland, V.E., McGrogan, D.C. and Ferguson, H.W., 19 Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus. J. Fish Dis. 20: 443–450 Pacha, R.E., 1968. Characteristics of Cytophaga psychrophila (Borg) isolated during outbreaks of bacterial cold-water disease. Appl. Microbiol. 16: 97-101.
  • Palti, Y., Vallejo, R.L., Welch, T.J., Evenhuis, J., Leeds, T.D., Gao, G., Liu, S., Rexroad III, C.E. and Wiens, G.D., 2003. Mapping of QTL for bacterial cold water disease resistance in rainbow trout. Aquaculture Conference Proceedings. P0296.
  • Rahman, M.H., Ototake, M., Iida, Y., Yokomizo, Y. and Nakanishi, T., 2000. Efficacy of oiladjuvanted vaccine for coldwater disease in ayu, Plecoglossus altivelis. Fish Pathol. 35: 199–203.
  • Shoemaker, C.A., Klesius, P.H. and Bricker, J. M., 19 Efficacy of a modified live Edwardsiella ictaluri vaccine in channel catfish as young as seven days post hatch. Aquaculture. 176: 189–193. Wakabayashi, H., Toyama, T. and Lida, T., 1994. A Study on serotyping of Cytophaga psychrophila isolated from fishes in Japan. Fish Pathol. 29: 101–104. Wood, E.M. and Yasutake, T.W., 19 Histopathology of fish, III. Peduncle (coldwater) disease. Prog. Fish-Cult. 18: 58-61.

Efficacy of a Formalin-Killed Vaccine against Flavobacterium psychrophilum in Rainbow Trout (Oncorhynchus mykiss)

Year 2014, Volume: 1 Issue: 2, 215 - 218, 26.07.2014

Abstract

Gökkuşağı alabalığı yavru sendromu için aşı geliştirilmesi tam hücre bakterinlerine dayalı olarak yapılmaktadır. Bu çalışmada, Flavobacterium psychrophilum’a karşı sonraki çalışmalarda aşı geliştirilmesinde kullanılacak aday suşun koruyucu etkileri araştırıldı. 30, 60 ve 90 dakika için 1×108 CFU/ml formalinle inaktive edilmiş hücrelere maruz kalan gökkuşağı alabalıklarının nisbi hayatta kalma oranlarındaki (RPS) değişimler belirlendi. RPS oranları sırasıyla %21.74, %34.78 ve %43.48 olarak kaydedildi. Sonuç olarak, bu suşun zayıf bir koruyucu etkiye sahip olduğu ve aşı yapımı için kullanımının uygun olmadığı kanaatine varıldı.

References

  • Amend, D.F., 1981. Potency testing of fish vaccines. Developments in Biological Standardization. 49: 447–454.
  • Borg, A.F., 1960. Studies on myxobacteria associated with diseases in salmonid fishes. J Wildl. Dis. 8: 1–85.
  • Dalsgaard, I. and Madsen, L., 2000. Bacterial Pathogens in Rainbow Trout Oncorhynchus mykiss (Walbaum), Reared At Danish Freshwater Farms. J. Fish Dis. 23: 199–209. Elsayed, E.E., Eissa, A.E. and Faisal, M., 2006. Isolation of Flavobacterium psychrophilum from sea lamprey, Petromyzon marinus L., with skin lesions in Lake Ontario. J. Fish Dis. 29: 629–632.
  • Holt, R.A., 1987. Cytophaga psychrophila, the causative agent of bacterial coldwater disease in salmonid fish. Doctoral dissertation. Oregon State University. Corvallis, Oregon.
  • Holt, R.A., Rohovec, J.S. and Fryer, J.L., 1993. Bacterial coldwater disease. In: Inglis V, Roberts R.J., Bromage N.R. (eds) Bacterial diseases of fish. Blackwell Scientific Publications. Oxford. 3–23 p.
  • Lida, Y. and Mizokami, A., 1996. Outbreaks of coldwater disease in wild ayu and pale chub. Fish Pathol. 31: 157–164
  • Kent, M.L., Groff, J.M., Morrison, J.K., Yasutake, W.T. and Holt, R.A., 1989. Spiral swimming behavior due to cranial and vertebral lesions associated with Cytophaga psychrophila infections in salmonid fishes. Diseases of Aquatic Organisms. 6: 11–16.
  • Klesius, P.H., Shoemaker, C.A. and Evans, J.J., 1999. Efficacy of a killed Streptococcus iniae vaccine in tilapia (Oreochromis niloticus). Bull. Eur. Assoc. Fish Pathol. 19(1): 39-41.
  • Kondo, M., Kawai, K., Okabe, M., Nakano, N. and Oshima, S.I., 2003. Efficacy of oral vaccine against bacterial coldwater disease in ayu Plecoglossus altivelis. Diseases of Aquatic Organisms. 55: 261–264
  • LaFrentz, B.R., LaPatra, S.E., Jones, G.R., Congleton, J.L., Sun, B. and. Cain, K.D., 2002. Characterization of serum and mucosal antibody responses and relative percent survival in rainbow trout, Oncorhynchus mykiss (Walbaum), following immunization and challenge with Flavobacterium psychrophilum. J. Fish Dis. 25: 703–713.
  • Lorenzen, E., 1994. Studies on Flexibacter pychrophilus in relation to rainbow trout fry syndrome (RTFS). National Veterinary Laboratory and Royal Veterinary and Agricultural University. Arhus. 1-25 p.
  • Lorenzen, E. and Olesen, N.J., 19 Characterization of isolates of Flavobacterium psychrophilum associated with coldwater disease or rainbow trout fry syndrome II: serological studies. Diseases of Aquatic Organisms. 31: 209–220. Lorenzen, E., Brudeseth, B.E., Wiklund, T. and Lorenzen, N., 2010. Immersion exposure of rainbow trout (Oncorhynchus mykiss) fry to wildtype Flavobacterium psychrophilum induces no mortality, but protects against later intraperitoneal challenge. Fish and Shellfish Immunology. 28: 440-444
  • Madetoja, J., Lönnström, L.G., Björkblom, C., Uluköy, G., Bylund, G., Syvertsen, C., Gravningen, K., Norderhus, E.A. and Wiklund, T., 2006. Efficacy of injection vaccines against Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. 29: 9–20.
  • Obach, K., and Laurencin, F.B., 1991. Vaccination of rainbow trout Oncorhynchus mykiss against the visceral form of coldwater disease. Dis. Aquat. Org. 12: 13–15.
  • Ostland, V.E., McGrogan, D.C. and Ferguson, H.W., 19 Cephalic osteochondritis and necrotic scleritis in intensively reared salmonids associated with Flexibacter psychrophilus. J. Fish Dis. 20: 443–450 Pacha, R.E., 1968. Characteristics of Cytophaga psychrophila (Borg) isolated during outbreaks of bacterial cold-water disease. Appl. Microbiol. 16: 97-101.
  • Palti, Y., Vallejo, R.L., Welch, T.J., Evenhuis, J., Leeds, T.D., Gao, G., Liu, S., Rexroad III, C.E. and Wiens, G.D., 2003. Mapping of QTL for bacterial cold water disease resistance in rainbow trout. Aquaculture Conference Proceedings. P0296.
  • Rahman, M.H., Ototake, M., Iida, Y., Yokomizo, Y. and Nakanishi, T., 2000. Efficacy of oiladjuvanted vaccine for coldwater disease in ayu, Plecoglossus altivelis. Fish Pathol. 35: 199–203.
  • Shoemaker, C.A., Klesius, P.H. and Bricker, J. M., 19 Efficacy of a modified live Edwardsiella ictaluri vaccine in channel catfish as young as seven days post hatch. Aquaculture. 176: 189–193. Wakabayashi, H., Toyama, T. and Lida, T., 1994. A Study on serotyping of Cytophaga psychrophila isolated from fishes in Japan. Fish Pathol. 29: 101–104. Wood, E.M. and Yasutake, T.W., 19 Histopathology of fish, III. Peduncle (coldwater) disease. Prog. Fish-Cult. 18: 58-61.
There are 18 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Muammer Kırıcı This is me

Ünal İspir This is me

Muhammed Atamanalp This is me

Publication Date July 26, 2014
Submission Date July 26, 2014
Published in Issue Year 2014 Volume: 1 Issue: 2

Cite

APA Kırıcı, M., İspir, Ü., & Atamanalp, M. (2014). Efficacy of a Formalin-Killed Vaccine against Flavobacterium psychrophilum in Rainbow Trout (Oncorhynchus mykiss). Turkish Journal of Agricultural and Natural Sciences, 1(2), 215-218.