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Management of Root-Knot Nematodes (Meloidogyne spp.) in Greenhouse Cucumbers Using Microbial Products

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1569 - 1573, 01.03.2014

Abstract

The environmental pollution is one of the major problems in the world. The decrease of agrochemicals like chemical pesticides and fertilizers is important to protect human health and the environment. The use of biocontrol agents against some root-knot nematodes Meloidogyne spp. is an alternative option to reduce environmental pollution. Paecilomyces lilacinus and Trichoderma viride are biocontrol fungi with a potential range of activity to control plant parasitic nematodes. Greenhouse experiments were conducted in 2013-2014 at the Maritsa Vegetable Crops Research Institute, Plovdiv to establish the biological activity of BioAct WG (Paecilomyces lilacinus strain 251) and Trichoderma viride strain T6 applied alone and in combination against root-knot nematodes (Meloidogyne spp.) in cucumber variety Defense F1. Two treatments with bioagents were carried out in a natural nematode population density: the first is at transplanting and the second - six weeks after it. All tested variants suppressed nematode reproduction and root galling and result in plant growth improvement compared to the control. The lowest rate of infestation and the highest total yield were established in the combination BioAct WG and Trichoderma viride strain T6. The micro-bioagents could be an effective mean to control root-knot nematodes, which results in vegetable production free of pesticides

References

  • Adegbite, A. A. and Adesiyan, S. O. 2005. Root Extracts of Plants to Control Root-Knot Nematode on Edible Soybean. World Journal of Agricultural Sciences 1 (1): 18- 21.
  • Affokpon, A., Coyne, D. L., Htay, C. C., Agbèdè, R. D., Lawouin, L. and Coosemans, J. 2011. Biocontrol Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biology & Biochemistry, 43, 600-608. of native
  • Amin, A. W. 2000. Efficacy of Arthrobotrys oligospora, Paecilomyces lilacinus and Pasteuria penetrans as potential biocontrol agents against tomato. Pakistan Journal of Nematology. 18:1-2, 29-33.
  • rhossiliensis, Meloidogyne incognita on
  • Atkins, S. D., Clark, I. M., Pande, S., Hirsch, P. R. and Kerry, B. K. 2005. The use of real- time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiol. Ecol. 51, 257–264
  • Dababat, A. A. and Sikora, R. A. 2007. Use of Trichoderma Trichoderma viride for the Biological Control of Meloidogyne incognita on Tomato. Jordan Journal of Agricultural Sciences, Vol.3:3, 297-308. and
  • Davide, R. G. 1985. Summary report on current status, Meloidogyne research in Region VI. In: Sasser, J.N., Carter, C.C. (Eds.), An Advanced Treatise on Meloidogyne. Biology and Control, vol. 1. North Carolina State University Graphics, Raleigh, North Carolina, 369-372.
  • Davide, R. G. and Zorilla, R. A. 1983. Evaluation of a fungus Paecilomyces lilacinus (Thom.) Samson, for the biological control of potato cyst nematode Globodera compared with some nematicides. Phillipine Agriculture, 66:397-404.
  • Duncan, D. 1955. Multiple range and multiple F- test. Biometrics 11: 1-42.
  • Ekanayake, H. M. R. K. and Jayasundara, N. J. 1994. Effect of Paecilomyces lilacinus and Beauveria bassiana in controlling Meloidogyne incognita on tomato in Sri Lanka. Nematologia Mediteranea, v. 22(1), 87-88.
  • Ganaie, M. A. and Khan, T. A. 2010. Biological Potential of Paecilomyces lilacinus on Pathogenesis of Meloidogyne javanica Infesting Tomato Plant. European jornal of Applied Sciences, 2 (2), 80-84.
  • Goswami, B. K. and Mittal, A. 2004. Management of root-knot nematode infecting tomato by Trichoderma viride and Phytopath. 57 (2): 235-236. Indian
  • Goswami, B. K., Pandey, R. K., Rathour, K. S., Bhattacharya, C. and Singh, L. 2006. Integrated compatible biocontrol agents along with mustard oil seed cake and furadan on Meloidogyne incognita infecting tomato plants. Journal of Zhejiang University SCIENCE B, 7(11):873-875. of some
  • Hussey, R. S. and Janssen, G. J. W. 2002. Root- knot nematode: Meloidogyne species. in J. L. Starr, R. Cook, and J. Bridge, eds. Plant Resistance to Parasitic Nematodes. Wallingford, UK: CAB International, 43– 70.
  • incognita by Paecilomyces lilacinus strain 251. – Biological Control 38: 179-187.
  • Karssen, G. and Moens, M. 2006. Root-knot nematodes.In: Perry, R.N. and Moens, M. (Eds). publishing, 59-90. .CABI
  • Kiewnick, S. 2004. Biological control of plant parasitic nematodes with Paecilomyces lilacinus, strain 251. In: Sikora, R.A., Gowen, S., Hauschild, R., Kiewnick, S. (Eds.), Multitrophic Interactions in Soil. IOBC/WPRS Bulletin 27, 133–143.
  • Kiewnick, S. and Sikora, R. A. 2004. Optimizing the efficacy of Paecilomyces lilacinus (strain 251) for the control of root-knot nematodes. Commun Agric Appl Biol Sci., 69(3):373-80.
  • Kiewnick, S. and Sikora, R. A. 2006. Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biological Control 38:179–187.
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  • Rodriguez-Kabana, R. and Pope, M. H. 1981. A simple incubation method for the extraction of nematodes from soil. Nematropica 11:175-185.
  • Sharon, E., Bar-Eyal, M., Chet, I., Herrera- Estrella, A., Kleifeld, O. and Spiegel, Y. 2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91:687-693.
  • Siddiqui, I. A., Qureshi, S. A., Sultana, V., Ehteshamul-Haque, S. and Ghaffar, A. 2000. Biological control of root rot-root knot disease complex of tomato. Plant and Soil, v. 227, 163-169.
  • Sikora, R. A. and Fernandez, E. 2005. Nematode parasites of vegetables, In: Luc, M., Sikora, R.A, Bridge, J. (Eds.). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI Publishing, Wallingford, UK, 319-392.
  • Wang, L., Yang, B. and Li, C. 1999. Evaluation of pathogenicity of parasitic fungi to root- knot nematodes. Scientia Silvae Sinicae, 35:3, 41-47.
  • Yang, X., He, Y., Chen, F. and Zheng, L. 2000. Isolation and selection of egg-mass fungi of Meloidogyne sp. in Fujian Provinse. Fugian Journal of Agricultural Sciences. 15:1, 12-15.
  • Zaki, F. A. 1994. Dose optimization of Paecilomyces lilacinus for the control of Meloidogyne Nematologia Mediteranea, v. 22(1), 45- 47. on tomato.

Management of Root-Knot Nematodes (Meloidogyne spp.) in Greenhouse Cucumbers Using Microbial Products

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1569 - 1573, 01.03.2014

Abstract

References

  • Adegbite, A. A. and Adesiyan, S. O. 2005. Root Extracts of Plants to Control Root-Knot Nematode on Edible Soybean. World Journal of Agricultural Sciences 1 (1): 18- 21.
  • Affokpon, A., Coyne, D. L., Htay, C. C., Agbèdè, R. D., Lawouin, L. and Coosemans, J. 2011. Biocontrol Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biology & Biochemistry, 43, 600-608. of native
  • Amin, A. W. 2000. Efficacy of Arthrobotrys oligospora, Paecilomyces lilacinus and Pasteuria penetrans as potential biocontrol agents against tomato. Pakistan Journal of Nematology. 18:1-2, 29-33.
  • rhossiliensis, Meloidogyne incognita on
  • Atkins, S. D., Clark, I. M., Pande, S., Hirsch, P. R. and Kerry, B. K. 2005. The use of real- time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiol. Ecol. 51, 257–264
  • Dababat, A. A. and Sikora, R. A. 2007. Use of Trichoderma Trichoderma viride for the Biological Control of Meloidogyne incognita on Tomato. Jordan Journal of Agricultural Sciences, Vol.3:3, 297-308. and
  • Davide, R. G. 1985. Summary report on current status, Meloidogyne research in Region VI. In: Sasser, J.N., Carter, C.C. (Eds.), An Advanced Treatise on Meloidogyne. Biology and Control, vol. 1. North Carolina State University Graphics, Raleigh, North Carolina, 369-372.
  • Davide, R. G. and Zorilla, R. A. 1983. Evaluation of a fungus Paecilomyces lilacinus (Thom.) Samson, for the biological control of potato cyst nematode Globodera compared with some nematicides. Phillipine Agriculture, 66:397-404.
  • Duncan, D. 1955. Multiple range and multiple F- test. Biometrics 11: 1-42.
  • Ekanayake, H. M. R. K. and Jayasundara, N. J. 1994. Effect of Paecilomyces lilacinus and Beauveria bassiana in controlling Meloidogyne incognita on tomato in Sri Lanka. Nematologia Mediteranea, v. 22(1), 87-88.
  • Ganaie, M. A. and Khan, T. A. 2010. Biological Potential of Paecilomyces lilacinus on Pathogenesis of Meloidogyne javanica Infesting Tomato Plant. European jornal of Applied Sciences, 2 (2), 80-84.
  • Goswami, B. K. and Mittal, A. 2004. Management of root-knot nematode infecting tomato by Trichoderma viride and Phytopath. 57 (2): 235-236. Indian
  • Goswami, B. K., Pandey, R. K., Rathour, K. S., Bhattacharya, C. and Singh, L. 2006. Integrated compatible biocontrol agents along with mustard oil seed cake and furadan on Meloidogyne incognita infecting tomato plants. Journal of Zhejiang University SCIENCE B, 7(11):873-875. of some
  • Hussey, R. S. and Janssen, G. J. W. 2002. Root- knot nematode: Meloidogyne species. in J. L. Starr, R. Cook, and J. Bridge, eds. Plant Resistance to Parasitic Nematodes. Wallingford, UK: CAB International, 43– 70.
  • incognita by Paecilomyces lilacinus strain 251. – Biological Control 38: 179-187.
  • Karssen, G. and Moens, M. 2006. Root-knot nematodes.In: Perry, R.N. and Moens, M. (Eds). publishing, 59-90. .CABI
  • Kiewnick, S. 2004. Biological control of plant parasitic nematodes with Paecilomyces lilacinus, strain 251. In: Sikora, R.A., Gowen, S., Hauschild, R., Kiewnick, S. (Eds.), Multitrophic Interactions in Soil. IOBC/WPRS Bulletin 27, 133–143.
  • Kiewnick, S. and Sikora, R. A. 2004. Optimizing the efficacy of Paecilomyces lilacinus (strain 251) for the control of root-knot nematodes. Commun Agric Appl Biol Sci., 69(3):373-80.
  • Kiewnick, S. and Sikora, R. A. 2006. Biological control of the root-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biological Control 38:179–187.
  • Lamovšek, J., Urek, G. and Trdan, S. 2013. Control Biological Nematodes Microbes against the Pests. Acta agriculturae Slovenica, 263 – 275.
  • Lara, J., Acosta, N., Betancourt, C., Vincente, N. and Rodriguez, R. 1996. Biological control of Meloidogyne incognita in tomato in Puerto Rico. Nematropica 26, 143–152.
  • Rodriguez-Kabana, R. and Pope, M. H. 1981. A simple incubation method for the extraction of nematodes from soil. Nematropica 11:175-185.
  • Sharon, E., Bar-Eyal, M., Chet, I., Herrera- Estrella, A., Kleifeld, O. and Spiegel, Y. 2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91:687-693.
  • Siddiqui, I. A., Qureshi, S. A., Sultana, V., Ehteshamul-Haque, S. and Ghaffar, A. 2000. Biological control of root rot-root knot disease complex of tomato. Plant and Soil, v. 227, 163-169.
  • Sikora, R. A. and Fernandez, E. 2005. Nematode parasites of vegetables, In: Luc, M., Sikora, R.A, Bridge, J. (Eds.). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI Publishing, Wallingford, UK, 319-392.
  • Wang, L., Yang, B. and Li, C. 1999. Evaluation of pathogenicity of parasitic fungi to root- knot nematodes. Scientia Silvae Sinicae, 35:3, 41-47.
  • Yang, X., He, Y., Chen, F. and Zheng, L. 2000. Isolation and selection of egg-mass fungi of Meloidogyne sp. in Fujian Provinse. Fugian Journal of Agricultural Sciences. 15:1, 12-15.
  • Zaki, F. A. 1994. Dose optimization of Paecilomyces lilacinus for the control of Meloidogyne Nematologia Mediteranea, v. 22(1), 45- 47. on tomato.
There are 28 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Vinelina Yankova This is me

Dima Markova This is me

Mladen Naıdenov This is me

Boyan Arnaoudov This is me

Publication Date March 1, 2014
Submission Date January 26, 2015
Published in Issue Year 2014 Volume: 1 Issue: Özel Sayı-2

Cite

APA Yankova, V., Markova, D., Naıdenov, M., Arnaoudov, B. (2014). Management of Root-Knot Nematodes (Meloidogyne spp.) in Greenhouse Cucumbers Using Microbial Products. Turkish Journal of Agricultural and Natural Sciences, 1(Özel Sayı-2), 1569-1573.