Isolation and Identification of Binucleat Rhizoctonia spp. from Wheat Field Soils in the Central Anatolia Region, Turkey

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1933 - 1938, 01.03.2014

Filiz Ünal F. Sara Dolar A. Faik Yıldırım Erkol Demirci

Abstract

Current study was conducted to identify the anastomosis groups and pathogenicity of binucleate Rhizoctonia species from soil samples in wheat production areas of Konya, Ankara, Yozgat, Eskişehir, Kırıkkale, Kayseri, Kırşehir, Nevşehir and Aksaray provinces in the Central Anatolia Regions during 2009-2012 growing season. One thousand and two hundred fifty six (1256) soil samples were collected from wheat fields and isolations were made from the soil that using colonization of bait tissue. Species and anastomosis groups identification was done according to the basis of hyphal and colony morphology, anastomosis reaction with known tester isolates. Pathogenicity test was conducted with agar-plate assay with all isolates. Fifty one isolates were identified as binucleate Rhizoctonia and those isolates were found to be belonged to AG A, AG C, AG D (R. cerealis), AG E, AG G, AG H, AG I and AG K. In consequence of the pathogenicity tests performed, the groups other than AG D were not found to be pathogenic on susceptible wheat cultivar. AG C identified in this study is first record for Turkey, and also AG A, AG E, AG G and AG H groups were determined to be first in the wheat field soils in Turkey

Keywords

Binucleat Rhizoctonia spp., anastomosis group, wheat, soil

Isolation and Identification of Binucleat Rhizoctonia spp. from Wheat Field Soils in the Central Anatolia Region, Turkey

Abstract

Bu araştırma 2009-2012 üretim sezonunda, İç Anadolu Bölgesi’nde Konya, Ankara, Yozgat, Eskişehir, Kırıkkale, Kayseri, Kırşehir, Nevşehir ve Aksaray İlleri buğday üretim alanlarından toplanan toprak örneklerindeki binükleat Rhizoctonia türlerinin anastomosis gruplarını ve patojenisitelerini belirlemek amacıyla yürütülmüştür. Buğday tarlalarından 1256 toprak örneği toplanmış ve toprak örneklerinden izolasyonlar tuzak bitki kullanılarak yapılmıştır. Tür ve anastomosis grupları, izolatların hif yapısı, koloni morfolojileri ve test izolatları ile anastomosis reaksiyonlarına göre yapılmıştır. Tüm izolatların patojenisite testleri petri denemeleri ile yapılmıştır. AG A, AG C, AG D (R. cerealis), AG E, AG G, AG H, AG I ve AG K anastomosis gruplarına ait 51 binükleat Rhizoctonia izolatı tanımlanmıştır. Hassas buğday çeşidi kullanılarak yapılan patojenisite çalışmaları sonucunda AG D dışındaki tüm izolatlar buğdayda patojen bulunmamıştır. AG C grubu Türkiye için ilk kayıttır. Ayrıca AG A, AG E, AG G ve AG H grupları ise Türkiye’de buğday tarla toprağında ilk kez tespit edilmiştir

Keywords

Binükleat Rhizoctonia spp., anastomosis grup, buğday, toprak

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