Çarkıfelek Çiçeğinin (Passiflora incarnata L.) Biyoaktif Kimyasallarının İncelenmesi

Year 2018, Volume: 5 Issue: 4, 471 - 476, 18.10.2018

Dilek Dülger Fatma Ergün Nazan Demır

https://doi.org/10.30910/turkjans.471285

Abstract

Bu çalışmada, çarkıfelek çiçeğinin (P. incarnata L.) proteaz enzim
aktivitesi ile uçucu organic bileşenleri belirlenerek bitkinin ilaç sektöründe
kullanılabilirliğinin belirlenmesine yönelik bir araştırma yapılmıştır. Çarkıfelek
çiçeğinde bulunan proteaz enziminin aktivitesini tayin etmek için %1’lik kazein
varlığında kazeinin sindirimi metoduna göre ölçüm alınmıştır. Okunan absorbans
değerleri ışığında bitkide proteolitik aktivite olduğu saptanmıştır. Ayrıca
bitkinin uçucu organik bileşikleri headspace GC/MSD cihazı ile analiz
edilmiştir. 5 g taze bitki alınarak 20 ml headspace vialine konulmuştur. Bitki
ekstraksiyon ve HP-5 MS UI kolonu kullanılarak 6890 GC-5975C MSD ile analiz
edilmiştir. Elde edilen sonuçlara göre bitkinin ilaç sanayinde kullanılabilir
olduğu bilimsel olarak belirlenmiştir. 

Keywords

Çarkıfelek çiçeği, Passiflora incarnata L., proteaz enzimi.

Investigation of Bioactive Chemicals of Passion Flower (Passiflora incarnata L.)

Abstract

In this study, a research was
conducted to determine the protease enzyme activity and the volatile organic
compounds of passion flower (P. incarnata
L.) to determine the usability of the plant in the pharmaceutical sector. In order
to determine the activity of the protease enzyme in passion flower,
measurements were taken according to the method of casein digestion in the
presence of 1% casein. In the light of the absorbance values, it is determined
that the plant has proteolytic activity. In addition, volatile organic
compounds of the plant were analyzed by headspace GC/MSD. 5 g fresh plant was
taken and put into 20 ml headspace vial. The plant was extracted and analyzed
with 6890 GC-5975C MSD using the HP-5 MS UI column. According to the results
obtained, it has been scientifically determined that the plant can be used in
the pharmaceutical industry. 

Keywords

Passion flower, Passiflora incarnata L, protease enzyme.

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