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Identification and phylogenetic differences of newly isolated Streptomyces sp.

Year 2021, Volume: 8 Issue: 3, 680 - 685, 26.07.2021
https://doi.org/10.30910/turkjans.891337

Abstract

Antimicrobial resistance and antibiotic use is a global health crisis today. Streptomyces is the source of most antimicrobials. Therefore, the similarities and differences within the species of Streptomyces are of great importance. In this study, isolation and identification of 3 different Streptomyces species isolated from sediment from the Dicle region were performed. Then, 16S rRNA gene sequence was obtained and the similarities and differences between species were revealed. As a result of the study, bacteria contained clorometric differences, when looking at the spore chain morphology in the SEM image, a knobby structure was formed, 16S rRNA sequences longer than 1000bp and in the phylogenetic tree created, 1 isolate was closely similar to the sequences obtained from GenBank (93 <), other It was observed that the two isolates differed (15 <). 2 different Streptomyces isolates obtained in line with these results are important in terms of antibiotic production and different resistance conditions in subsequent studies.

References

  • Ayari A, Morakchi H, Djamila KG (2012). Identification and antifungal activity of Streptomyces sp. S72 isolated from Lake Oubeira sediments in North-East of Algeria. African Journal of Biotechnology 11 (2): 305-311. doi: 10.5897/AJB10.2345.
  • Azarova IE, Klyosova EY, Kolomoets II, Azarova VA, Ivakin VE et al. (2020). Polymorphisms of the Gene Encoding Cytochrome b-245 Beta Chain of NADPH Oxidase: Relationship with Redox Homeostasis Markers and Risk of Type 2 Diabetes Mellitus. Russian Journal of Genetics 56 (7): 856-862. doi: 10.1134/S1022795420070017.
  • Budak S, Duranyıldız İ, Yetiş Ü (1997). Ulusal Çevre Eylem Planı Su Kaynakları Yönetimi. Devlet Su İşleri Genel Müdürlüğü. Ankara,
  • Elliot MA, Buttner MJ, Nodwell JR (2008). Multicellular development in Streptomyces In: D Whitworth, editors. Myxobacteria: Multicellularity and Differentiation. American Society for Microbiology 419–439. doi: 10.1128/9781555815677.ch24
  • Ergun Z, Gürbüz A (2012). Geomorphological, Climatic and Anthropogenic Affects on the Sediment Transport Amounts of the Euphrates and Tigris Rivers 65th Geological Congress of Turkey: Ankara, Turkey pp. 574-575.
  • Gebreyohannes G, Moges F, Sahile S, Raja N (2013). Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pacific Journal of Tropical Biomedicine 3 (6): 426-435. doi: 10.1016/S2221-1691(13)60092-1.
  • Horinouchi S (2002). A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus. Frontiers in Bioscience 7: 2045-2057.
  • Laura RR, Boghos SM, Maria MG, Luis D (2018). Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America). BioMed Research International doi: 10.1155/2018/2839356.
  • Law JW-F, Ser H-L, Khan TM, Chuah L-H, Pusparajah P et al. (2017). The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Frontiers in Microbiology 8 (3) doi: 10.3389/fmicb.2017.00003
  • Mullowney MW, Hwang CH, Newsome AG, Wei X, Tanouye U et al. (2015). Diaza-anthracene antibiotics from a freshwater-derived actinomycete with selective antibacterial activity toward Mycobacterium tuberculosis. ACS Infectious Diseases 1 (4): 168-174. doi: 10.1021/acsinfecdis.5b00005.
  • Pandey B, Ghimire P, Agrawal VP (2004). International Conference on the Great Himalayas: Climate, Health, Ecology, Management and Conservation, Kathmandu. Organized by Kathmandu University and the Aquatic Ecosystem Health and Management Society, Canada.
  • Riquelme C, Hathaway JJM, Dapkevicius MLNE, Miller AZ, Kooser A et al. (2015). Actinobacterial Diversity in Volcanic Caves and Associated Geomicrobiological Interactions. Frontiers in Microbiology 6:1342. doi: 10.3389/fmicb.2015.01342.
  • Salehghamari E, Nasrollahzadeh Z, Tahmaseb M, Amoozegar MA (2019). Pectinase enzyme from Streptomyces coelicoflavus GIAL86 isolated from Meyghan Salt Lake, Arak, Iran. International Journal of Aquatic Biology 7 (2): 106-111. doi: 10.22034/ijab.v7i2.481.
  • Seçkin H, Önalan Ş (2020). Phylogenetic diversity of nocardia sp. Obtained from different water environments. Research Journal of Biology Sciences 13 (2): 37-44.
  • Ser H-L, Tan LT-H, Law JW-F, Chan K-G, Duangjai A et al. (2017). Focused review: cytotoxic and antioxidant potentials of mangrove-derived Streptomyces. Frontiers in Microbiology 8: 2065. doi: 10.3389/fmicb.2017.02065.
  • Shivlata L, Tulasi S (2015). Thermophilic and alkaliphilic Actinobacteria: biology and potential applications. Frontiers in Microbiology 6: 1014. doi: 10.3389/fmicb.2015.01014.
  • Sikkema‐Raddatz B, Johansson LF, de Boer EN, Almomani R, Boven LG et al. (2013). Targeted next‐generation sequencing can replace Sanger sequencing in clinical diagnostics. Human mutation 34 (7): 1035-1042. doi: 10.1002/humu.22332.
  • Tokatli A, Idil O, Veyisoglu A, Saygin H, Guven K et al. (2020). Streptomyces boluensis sp. nov., isolated from lake sediment. Archives of Microbiology 202 (8): 2303-2309. doi: 10.1007/s00203-020-01901-3.

İzole edilen yeni Streptomyces türlerinin tanımlanması ve filogenetik farklılıkları

Year 2021, Volume: 8 Issue: 3, 680 - 685, 26.07.2021
https://doi.org/10.30910/turkjans.891337

Abstract

Antimikrobiyal direnç ve antibiyotik kullanımı günümüzde küresel bir sağlık krizidir. Streptomyces, antimikrobiyallerin çoğunun kaynağıdır. Bu nedenle, Streptomyces türleri arasındaki benzerlikler ve farklılıklar büyük önem taşımaktadır. Bu çalışmada, Dicle bölgesinden tortudan izole edilen 3 farklı Streptomyces türünün izolasyonu ve tanımlanması yapılmıştır. Daha sonra 16S rRNA gen dizisi elde edildi ve türler arasındaki benzerlikler ve farklılıklar ortaya çıktı. Çalışma sonucunda bakteriler klorometrik farklılıklar içeriyordu, SEM görüntüsünde spor zinciri morfolojisine bakıldığında yumru bir yapı oluştu, 16S rRNA dizileri 1000bp'den uzun ve oluşturulan filogenetik ağaçta 1 izolat ile yakından benzerlik gösterdi. GenBank'tan elde edilen diziler (93 <), diğer iki izolatın farklı olduğu (15 <) gözlendi. Bu sonuçlar doğrultusunda elde edilen 2 farklı Streptomyces izolatı, sonraki çalışmalarda antibiyotik üretimi ve farklı direnç koşulları açısından önemlidir.

References

  • Ayari A, Morakchi H, Djamila KG (2012). Identification and antifungal activity of Streptomyces sp. S72 isolated from Lake Oubeira sediments in North-East of Algeria. African Journal of Biotechnology 11 (2): 305-311. doi: 10.5897/AJB10.2345.
  • Azarova IE, Klyosova EY, Kolomoets II, Azarova VA, Ivakin VE et al. (2020). Polymorphisms of the Gene Encoding Cytochrome b-245 Beta Chain of NADPH Oxidase: Relationship with Redox Homeostasis Markers and Risk of Type 2 Diabetes Mellitus. Russian Journal of Genetics 56 (7): 856-862. doi: 10.1134/S1022795420070017.
  • Budak S, Duranyıldız İ, Yetiş Ü (1997). Ulusal Çevre Eylem Planı Su Kaynakları Yönetimi. Devlet Su İşleri Genel Müdürlüğü. Ankara,
  • Elliot MA, Buttner MJ, Nodwell JR (2008). Multicellular development in Streptomyces In: D Whitworth, editors. Myxobacteria: Multicellularity and Differentiation. American Society for Microbiology 419–439. doi: 10.1128/9781555815677.ch24
  • Ergun Z, Gürbüz A (2012). Geomorphological, Climatic and Anthropogenic Affects on the Sediment Transport Amounts of the Euphrates and Tigris Rivers 65th Geological Congress of Turkey: Ankara, Turkey pp. 574-575.
  • Gebreyohannes G, Moges F, Sahile S, Raja N (2013). Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pacific Journal of Tropical Biomedicine 3 (6): 426-435. doi: 10.1016/S2221-1691(13)60092-1.
  • Horinouchi S (2002). A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus. Frontiers in Bioscience 7: 2045-2057.
  • Laura RR, Boghos SM, Maria MG, Luis D (2018). Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America). BioMed Research International doi: 10.1155/2018/2839356.
  • Law JW-F, Ser H-L, Khan TM, Chuah L-H, Pusparajah P et al. (2017). The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Frontiers in Microbiology 8 (3) doi: 10.3389/fmicb.2017.00003
  • Mullowney MW, Hwang CH, Newsome AG, Wei X, Tanouye U et al. (2015). Diaza-anthracene antibiotics from a freshwater-derived actinomycete with selective antibacterial activity toward Mycobacterium tuberculosis. ACS Infectious Diseases 1 (4): 168-174. doi: 10.1021/acsinfecdis.5b00005.
  • Pandey B, Ghimire P, Agrawal VP (2004). International Conference on the Great Himalayas: Climate, Health, Ecology, Management and Conservation, Kathmandu. Organized by Kathmandu University and the Aquatic Ecosystem Health and Management Society, Canada.
  • Riquelme C, Hathaway JJM, Dapkevicius MLNE, Miller AZ, Kooser A et al. (2015). Actinobacterial Diversity in Volcanic Caves and Associated Geomicrobiological Interactions. Frontiers in Microbiology 6:1342. doi: 10.3389/fmicb.2015.01342.
  • Salehghamari E, Nasrollahzadeh Z, Tahmaseb M, Amoozegar MA (2019). Pectinase enzyme from Streptomyces coelicoflavus GIAL86 isolated from Meyghan Salt Lake, Arak, Iran. International Journal of Aquatic Biology 7 (2): 106-111. doi: 10.22034/ijab.v7i2.481.
  • Seçkin H, Önalan Ş (2020). Phylogenetic diversity of nocardia sp. Obtained from different water environments. Research Journal of Biology Sciences 13 (2): 37-44.
  • Ser H-L, Tan LT-H, Law JW-F, Chan K-G, Duangjai A et al. (2017). Focused review: cytotoxic and antioxidant potentials of mangrove-derived Streptomyces. Frontiers in Microbiology 8: 2065. doi: 10.3389/fmicb.2017.02065.
  • Shivlata L, Tulasi S (2015). Thermophilic and alkaliphilic Actinobacteria: biology and potential applications. Frontiers in Microbiology 6: 1014. doi: 10.3389/fmicb.2015.01014.
  • Sikkema‐Raddatz B, Johansson LF, de Boer EN, Almomani R, Boven LG et al. (2013). Targeted next‐generation sequencing can replace Sanger sequencing in clinical diagnostics. Human mutation 34 (7): 1035-1042. doi: 10.1002/humu.22332.
  • Tokatli A, Idil O, Veyisoglu A, Saygin H, Guven K et al. (2020). Streptomyces boluensis sp. nov., isolated from lake sediment. Archives of Microbiology 202 (8): 2303-2309. doi: 10.1007/s00203-020-01901-3.
There are 18 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Şükrü Önalan 0000-0003-0058-5232

Hamdullah Seçkin 0000-0003-3884-4121

Publication Date July 26, 2021
Submission Date March 4, 2021
Published in Issue Year 2021 Volume: 8 Issue: 3

Cite

APA Önalan, Ş., & Seçkin, H. (2021). Identification and phylogenetic differences of newly isolated Streptomyces sp. Türk Tarım Ve Doğa Bilimleri Dergisi, 8(3), 680-685. https://doi.org/10.30910/turkjans.891337