Identification and phylogenetic differences of newly isolated Streptomyces sp.

Year 2021, Volume: 8 Issue: 3, 680 - 685, 26.07.2021

Şükrü Önalan , Hamdullah Seçkin

https://doi.org/10.30910/turkjans.891337

Cited By: 1

Abstract

Antimicrobial resistance and antibiotic use is a global health crisis today. Streptomyces is the source of most antimicrobials. Therefore, the similarities and differences within the species of Streptomyces are of great importance. In this study, isolation and identification of 3 different Streptomyces species isolated from sediment from the Dicle region were performed. Then, 16S rRNA gene sequence was obtained and the similarities and differences between species were revealed. As a result of the study, bacteria contained clorometric differences, when looking at the spore chain morphology in the SEM image, a knobby structure was formed, 16S rRNA sequences longer than 1000bp and in the phylogenetic tree created, 1 isolate was closely similar to the sequences obtained from GenBank (93 <), other It was observed that the two isolates differed (15 <). 2 different Streptomyces isolates obtained in line with these results are important in terms of antibiotic production and different resistance conditions in subsequent studies.

Keywords

Real-Time PCR, Sequencing, 16S rRNA gene, Streptomyces sp

İzole edilen yeni Streptomyces türlerinin tanımlanması ve filogenetik farklılıkları

Abstract

Antimikrobiyal direnç ve antibiyotik kullanımı günümüzde küresel bir sağlık krizidir. Streptomyces, antimikrobiyallerin çoğunun kaynağıdır. Bu nedenle, Streptomyces türleri arasındaki benzerlikler ve farklılıklar büyük önem taşımaktadır. Bu çalışmada, Dicle bölgesinden tortudan izole edilen 3 farklı Streptomyces türünün izolasyonu ve tanımlanması yapılmıştır. Daha sonra 16S rRNA gen dizisi elde edildi ve türler arasındaki benzerlikler ve farklılıklar ortaya çıktı. Çalışma sonucunda bakteriler klorometrik farklılıklar içeriyordu, SEM görüntüsünde spor zinciri morfolojisine bakıldığında yumru bir yapı oluştu, 16S rRNA dizileri 1000bp'den uzun ve oluşturulan filogenetik ağaçta 1 izolat ile yakından benzerlik gösterdi. GenBank'tan elde edilen diziler (93 <), diğer iki izolatın farklı olduğu (15 <) gözlendi. Bu sonuçlar doğrultusunda elde edilen 2 farklı Streptomyces izolatı, sonraki çalışmalarda antibiyotik üretimi ve farklı direnç koşulları açısından önemlidir.

Keywords

Real-Time PCR, Sekans, 16S rRNA gene, Streptomyces

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