SEASONAL CHANGE OF CHLOROPHYLL CONTENT (SPAD VALUE) IN SOME TREE AND SHRUB SPECIES

Year 2020, Volume: 4 Issue: 2, 245 - 256, 24.10.2020

Fahrettin Atar , Deniz Güney , Ali Bayraktar , Nebahat Yıldırım , İbrahim Turna

https://doi.org/10.32328/turkjforsci.711389

Cited By: 9

Abstract

Chlorophyll, which is of vital importance for living things, is the main active ingredient of photosynthesis, and the amount of chlorophyll in the plant can show very sensitive responses to various environmental factors, especially site conditions and plant species. In this study, it was aimed to determine the change of chlorophyll content (SPAD value) at the beginning (20 May) and the end (15 October) of growth period in some native and exotic tree and shrub species in Kanuni campus of Karadeniz Technical University and to reveal the differences between the species. Within the scope of the study, measurements were made on a total of 20 species including five native (Quercus hartwissiana Steven, Fagus orientalis Lipsky, Ulmus minör Mill., Liquidambar orientalis Mill., Quercus pubescens Willd.) and seven exotic (Eucalyptus camadulensis Dehnh., Ginkgo biloba L., Quercus castaneifolia C.A.Mey., Cinnamomum camphora (L.) Sieb., Acer negundo L., Quercus rubra L., Aesculus hippocastanum L.) tree species and seven native (Cercis siliquastrum L., Laurus nobilis L., Osmanthus decorus (Boiss.&Balansa) Kasapligil, Laurocerasus officinalis M.Roem., Ostrya carpinifolia Scop., Corylus avellana L., Arbutus unedo L.) and one exotic (Weigela coraensis Thunb.) shrub species. Chlorophyll content was determined with four replications by portable chlorophyll meter (Minolta SPAD-502, Osaka, Japan) which indirectly measures the amount of chlorophyll in the leaf. The data obtained were subjected to Wilcoxon test, variance analysis (one-way ANOVA) and Duncan’s test by using SPSS 23.0 statistical program. As a result of the study, it was determined that there are statistically significant differences (p<0.05) between SPAD values of May and October of species except Aesculus hippocastanum, Laurus nobilis, Cinnamomum camphora, Eucalyptus camaldulensis and Liquidambar orientalis. In addition, it was found that there are significant differences at 99% confidence level among the species in both periods. As a result of Duncan’s test, nine different groups occurred in May measurements and 15 different groups occurred in October measurements. While the average SPAD value was the highest (78.6 and 70.6, respectively) in Laurocerasus officinalis in both periods, the lowest was in Acer negundo (25.0) in May and Quercus hartwissiana (24.0) in October.

Keywords

Species, Photosynthesis, Chlorophyll, Minolta

BAZI AĞAÇ VE ÇALI TÜRLERİNDE KLOROFİL İÇERİĞİNİN (SPAD DEĞERİ) MEVSİMSEL DEĞİŞİMİ

Abstract

Canlılar için hayati öneme sahip olan klorofil, fotosentez olayının başlıca etken maddesi olup, bitkideki klorofil miktarı yetişme yeri koşulları ve bitki türü başta olmak üzere çeşitli çevresel faktörlere karşı oldukça hassas tepkiler gösterebilmektedir. Bu çalışmada, Karadeniz Teknik Üniversitesi Kanuni yerleşkesi içerisinde bulunan bazı doğal egzotik ağaç ve çalı türlerinde klorofil içeriğinin (SPAD değeri) büyüme dönemi başlangıcında (20 Mayıs) ve sonundaki (15 Ekim) değişiminin belirlenmesi ve türler arasındaki farkların ortaya konması amaçlanmıştır. Çalışma kapsamında Kanuni yerleşkesinde yer alan beş adet doğal (Quercus hartwissiana Steven, Fagus orientalis Lipsky, Ulmus minör Mill., Liquidambar orientalis Mill., Quercus pubescens Willd.) ve yedi adet egzotik (Eucalyptus camadulensis Dehnh., Ginkgo biloba L., Quercus castaneifolia C.A.Mey., Cinnamomum camphora (L.) Sieb., Acer negundo L., Quercus rubra L., Aesculus hippocastanum L.) ağaç türü ile yedi adet doğal (Cercis siliquastrum L., Laurus nobilis L., Osmanthus decorus (Boiss.&Balansa) Kasapligil, Laurocerasus officinalis M.Roem., Ostrya carpinifolia Scop., Corylus avellana L., Arbutus unedo L.) ve bir adet egzotik (Weigela coraensis Thunb.) çalı türü olmak üzere toplam 20 adet türde ölçümler gerçekleştirilmiştir. Klorofil içeriği, yapraktaki klorofil miktarını dolaylı olarak ölçen, taşınabilir klorofil metre cihazı (Minolta SPAD-502, Osaka, Japan) ile 4 tekerrürlü olarak tespit edilmiştir. Elde edilen veriler SPSS 23.0 istatistik programı yardımıyla değerlendirilerek, Wilcoxon testi, varyans analizi (one-way Anova) ve Duncan’s testi yapılmıştır. Çalışma sonucunda Aesculus hippocastanum, Laurus nobilis, Cinnamomum camphora, Eucalyptus camaldulensis ve Liquidambar orientalis dışındaki türlerin Mayıs ve Ekim ayına ait SPAD değerleri arasında istatistiksel olarak anlamlı (p<0.05) farklılıklar olduğu belirlenmiştir. Ayrıca, her iki dönemde de türler arasında %99 güven düzeyinde anlamlı farklılıklar bulunduğu tespit edilmiştir. Duncan’s testi sonucunda Mayıs ayı ölçümlerinde dokuz farklı grup, ekim ayı ölçümlerinde 15 farklı grup meydana geldiği saptanmıştır. Ortalama SPAD değeri her iki dönemde de Laurocerasus officinalis türünde en yüksek (sırasıyla 78.6 ve 70.6) iken en düşük değer Mayıs ayında Acer negundo (25.0), Ekim ayında Quercus hartwissiana (24.0) türünde belirlenmiştir.

Keywords

Tür, Fotosentez, Klorofil, Minolta

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