Antioxidant, Anti-Lipid Peroxidation and Antimicrobial Effect of Heracleum persicum

Abstract

This study was conducted to determine the antioxidant activity, lipid peroxidation inhibitory effect and antimicrobial properties of Heracleum persicum plant. DPPH (2, 2-diphenyl, 1-picrylhydrazyl) method was used to determine antioxidant activity, and the Folin method was used to determine total phenolic content. Disc diffusion method was applied to determine antimicrobial activity. In the study, the antioxidant activity value of H. persicum was determined as 5.36 mg mL-1, the total phenolic content was 20.84 mg GAE mL-1 and the total flavonoid content was 12.35 mg QE mL-1. The plant's inhibition against lipid peroxidation was measured as 3.63 mg mL-1. Its antioxidant activity and inhibitory effect against lipid peroxidation were lower than butylated hydroxyanisole (BHA) in both analyses (p<0.05). The plant extract was found to be effective against Bacillus subtilis, Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus bacteria. As a result, the antioxidant properties, antimicrobial and anti-lipid peroxidation effects of H. persicum plant have revealed that this plant has an important potential for use in medicine and pharmacology, as well as for consumption as food.

Keywords

• Antimicrobial effect
• bioactive compounds
• Heracleum persicum
• lipid peroxidation
• flavonoids
• phenols

References

1. Akbaribazm, M., Goodarzi, N., Rahimi, M., Naseri, L., Khazaei, M., 2021. Anti-inflammatory, anti-oxidative and anti-apoptotic effects of Heracleum persicum L. extract on rats with gentamicin-induced nephrotoxicity. Asian Pacific Journal of Tropical Biomedicine, 11(2): 47-58.
2. Al-Menhali, A.S., Banu, S., Angelova, P.R., Barcaru, A., Horvatovich, P., Abramov, A.Y., Jaganjac, M., 2020. Lipid peroxidation is involved in calcium dependent upregulation of mitochondrial metabolism in skeletal muscle. Biochimica et Biophysica Acta-General Subjects, 1864: 129487.
3. Araújo, J.S.C., Castilho, A.R.F., Lira, A.B., Pereira, A.V., Azevêdo, T.K.B., Brito Costa, E.M.M., Pereira, M.S.V., Pessôah, H.L.F., Pereira, J.V., 2018. Antibacterial activity against cariogenic bacteria and cytotoxic and genotoxic potential of Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Brenan extracts. Archives of Oral Biology, 85: 113-119.
4. Aruoma, O.I., 2003. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutation Research, 524: 9-20.
5. Çoruh, N., Sagdıçoglu Celep, A.G., Özgökçe, F., 2007. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chemistry, 100(3): 1237-1242.
6. Dehghan, H., Sarrafi, Y., Salehi, P., 2016. Antioxidant and antidiabetic activities of 11 herbal plants from Hyrcania region, Iran. Journal of Food and Drug Analysis, 24(1): 179-188.
7. Del Rio, D., Rodriguez-Mateos, A., Spencer, J.P.E., Tognolini, M., Borges, G., Crozier, A., 2013. Dietary (poly) phenolics in human health: Structures, bioavailability, and evidence of protective effects against chronic diseases. Antioxidants & Redox Signaling, 18(14): 1818-1892.
8. Doğan, D., Meydan, İ., 2021. Antioxidant effect of Achillea biebersteinii in different extracts and extinguhishing activity on DPPH. Journal of Agriculture, 4(1): 1-9.

9. Frey, F.M., Meyers, R., 2010. Antibacterial activity of traditional medicinal plants used by Haudenosaunee peoples of New York State. BMC Complementary and Alternative Medicine, 10: 64.
10. Grassi, D., Desideri, G., Ferri, C., 2010. Flavonoids: Antioxidants against atherosclerosis. Nutrients, 2(8): 889-902.
11. Hanachi, P., Gharari, Z., Sadeghinia, H., Walker, T. R., 2022. Synthesis of bioactive silver nanoparticles with eco-friendly processes using Heracleum persicum stem extract and evaluation of their antioxidant, antibacterial, anticancer and apoptotic potential. Journal of Molecular Structure, 1265: 133325.
12. Karan, T., Genc, N., 2018. Antioxidant activities of Heracleum platytaenium extracts and essential oil. Turkish Journal of Agriculture - Food Science and Technology, 6(9): 1274-1278.
13. Karimi, F., Rezaei-Savadkouhi, N., Uçar, M., Aygun, A., Tiri, R.N.E., Meydan, I., Aghapour, E., Seckin, H., Berikten, D., Gur, T., Sen, F., 2022. Efficient green photocatalyst of silver-based palladium nanoparticles for methyle orange photodegradation, investigation of lipid peroxidation inhibition, antimicrobial, and antioxidant activity. Food and Chemical Toxicology, 169: 113406.
14. Kenari, R.E., Amiri, Z.R., Motamedzadegan, A., Milani, J.M., Farmani, J., Farahmandfar, R., 2020. Optimization of Iranian golpar (Heracleum persicum) extract encapsulation using sage (Salvia macrosiphon) seed gum: chitosan as a wall materials and its effect on the shelf life of soybean oil during storage. Journal of Food Measurement and Characterization, 14: 2828-2839.
15. Koçak, Y., Oto, G., Meydan, İ., Seçkin, H., 2020. Investigation of total flavonoid, DPPH radical scavenging, lipid peroxidation and antimicrobial activity of Allium schoenoprasum L. plant growing in Van Region. Yuzuncu Yil University Journal of Agricultural Sciences, 30(1): 147-155.
16. Kousha, A., Bayat, M., 2012. Bactericidal and fungicidal activity of methanolic extracts of Heracleum persicum Desf. ex fischer against some aquatic and terrestrial animal pathogens. International Journal of Pharmacology, 8(7): 652-656.
17. Maddu, N., 2019. Diseases Related to Types of Free Radicals, Antioxidants. Intechopen, UK.
18. Majidi, Z., Lamardi, S.S., 2018. Phytochemistry and biological activities of Heracleum persicum: A review. Journal of Integrative Medicine, 16(4): 223-235.
19. Majidi, Z., Sadati Lamardi, S.N., Mohajjel-Nayebi, A., Vatankhah, A.M., Asnaashari, S., Zakeri-Milani, P., 2020. Effects of Heracleum persicum hydroalcoholic extract on insulin, serum anti-oxidant enzymes, glucose, and lipid profiles in alloxan-induced diabetic rats. Iranian Journal of Medical Sciences, 45(3): 199-206.
20. Mazandarani, M., Makari, S., Bajian, G.R., Moghaddam P.Z., Abrodi, M., 2011. Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech.F. in Golestan province of Iran. Iranian Journal of Plant Physiology, 2(2): 381-388.
21. Meydan, I., Kızıl, G., Demir, H., Çeken Toptancı, B., Kızıl, M., 2020. In vitro DNA damage, protein oxidation protective activity and antioxidant potentials of almond fruit (Amygdalus trichamygdalus) parts (hull and drupe) using soxhlet ethanol extraction. Advances in Traditional Medicine, 20(4): 571-579.
22. Nazemi, A., Hashemi, M., Khataminejad, M., Pourshamsian, K., 2005. Study on antibacterial effect of methanol and aquatic extract of Heracleum persicum plant. Medical Sciences Journal of Islamic Azad University, 15(2): 91-94.
23. Nickavar, B., Abolhasani, F.A., 2009. Screening of antioxidant properties of seven Umbelliferae fruits from Iran. Pakistan Journal of Pharmaceutical Sciences, 22(1): 30-35.
24. Ocal, M., Altunoglu, Y.C., Angeloni, S., Mustafa, A.M., Caprioli, G., Zengin, G., Paksoy, M.Y., Baloglu, M.C., 2022. Comparative content, biological and anticancer activities of Heracleum humile extracts obtained by ultrasound-assisted extraction method. Chemistry & Biodiversity, 19(7): e202101040.
25. Okumuş, E., 2023. Effect of different drying processes on antioxidant and antidiabetic properties of pomegranate press wastes. Yuzuncu Yil University Journal of the Institute of Natural & Applied Sciences, 28(1): 113-120.
26. Özyazıcı, M.A., 2022. Legume forage crops with medicinal value and their secondary metabolite contents: Medicago sp., Onobrychis sp., Melilotus sp., and Lupinus sp. In: G. Özyazıcı (Ed.), New Development on Medicinal and Aromatic Plants-II, İksad Publishing House, Ankara, Türkiye, pp. 33-70.
27. Özyazıcı, M.A., 2023. Legume forage crops with medicinal value and their secondary metabolite contents: Vicia sp., Trifolium sp., Trigonella foenum-graecum L., Lathyrus sp. In: G. Özyazıcı (Ed.), Current Studies on Medicınal Plants-I, İksad Publishing House, Ankara, Türkiye, pp. 57-94.
28. Pyo, Y.H., Lee, T.C., Logendra, L., Rosen, R.T., 2004. Antioxidant activity and phenolic compounds of swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chemistry, 85(1): 19-26.
29. Rahimi, A., Özyazıcı, G., Ahmadi, F., Pourakbar, L., 2019. Evaluation of some phytochemical properties of three medicinal plants from northwest of Iran. Journal of Biological & Environmental Sciences, 13(39): 151-159.
30. Razzaghi-Abyaneh, M., Chang, P.K., Shams-Ghahfarokhi, M., Rai, M., 2014. Global health issues of aflatoxins in food and agriculture: Challenges and opportunities. Frontiers in Microbiology, 5: 420.
31. Rodríguez-Rojo, S., Visentin, A., Maestri, D., Cocero, M.J., 2012. Assisted extraction of rosemary antioxidants with green solvents. Journal of Food Engineering, 109(1): 98-103.
32. Seçkin, H., Meydan, I., 2022. Synthesis and characterization of Sophora alopecuroides L. green synthesized of Ag nanoparticles for the antioxidant, antimicrobial and DNA damage prevention activity. Brazilian Journal of Pharmaceutical Sciences, 58: 1-11.
33. Shariatifar, N., Mostaghim, T., Afshar, A., Mohammadpourfard, I., Sayadi, M., Rezaei, M., 2017. Antibacterial properties of essential oil of Heracleum persicum (Golpar) and foodborne pathogens. International Journal of Enteric Pathogens, 5(2): 41-44.
34. Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphotungustic acid reagents. American Journal of Enology and Viticulture, 16: 144-158.
35. Souri, F., Farsam, H., Sarkheil, P., Ebadi, F., 2004. Antioxidant activity of some furanocoumarins isolated from Heracleum persicum. Pharmaceutical Biology, 42(6): 396-399.
36. Zhishen, J., Mengcheng, T., Jianming, W., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4): 55-559.