Eucalyptus grandis YABBY Transkripsiyon Faktörlerinin Genom Bazında Analizi

Abstract

YABBY gen ailesi bitki transkripsiyon faktörlerinden biridir. Bu çalışmanın amacı Eucalyptus grandis bitkisinde in siliko olarak YABBY gen ailesinin üyelerini genom çapında belirlemek ve karakterize etmektir. Bu çalışmada çeşitli in siliko yöntemler kullanılmıştır. Ökaliptus genomunda 6 Egra-YABBY proteini tespit edilmiştir. Bu proteinler 18.39 ile 32.38 kDa ağırlığına sahip olup, 168-290 arasında değişen amino asitten oluşmuştur. İzoelektrik noktaları 5.54 (Egra-YABBY- 2) ile 9.92 (Egra-YABBY- 6) arasındadır. Egra-YABBY genleri arasında tahmini belirlenen ekzonların sayısı en düşük 6, en yüksek 7 olarak elde edilmiştir. Filogenetik analizler sonucunda Egra- YABBY proteinleri ile Arabidopsis thaliana ve Vitis vinifera türlerinin proteinleri 5 ana grupta (FIL, INO, CRC, YAB2 ve YAB5) kümelenmiştir. Egra-YABBY-4/Egra-YABBY-5 genleri arasında segmental duplikasyon tespit edilmiştir. Egra-YABBY genlerinin ifade profilleri bu genlerin farklı dokularda ifade olduğunu ortaya çıkarmış ve bitkinin gelişim süresince çeşitli fizyolojik işlevlerde görev alabildiklerini de göstermiştir. Bu çalışmanın sonuçları, ökaliptus bitkisinde YABBY gen ailesinin moleküler temellerinin daha fazla anlaşılması için potansiyel biyoteknolojik kaynak ve ilave bilgiler sağlayacaktır.

Keywords

• in siliko analiz,YABBY,Domain,ökaliptus,transkripsiyon faktörü,YABBY

Genome-Wide Analysis of Eucalyptus grandis YABBY Transcription Factors

Abstract

YABBY gene family is one of the plant transcription factors. The aim of this study was to characterize and define the YABBY gene family members in silico, in Eucalyptus grandis genome. In this study, various in silico methods was used. A total of 6 Egra-YABBY proteins were discovered in Eucalyptus grandis genome. The molecular weight and length of these proteins ranged from 18.39 kDa to 32.38 kDa and based from 168 to 290 amino acids. Isoelectric points (pIs) of YABBY proteins were between 5.54 (Egra-YABBY- 2) and 9.92 (Egra-YABBY- 6). The estimated number of exons found among the Egra-YABBY genes was the lowest of 6 and the highest of 7. The results of the phylogenetic analysis showed that YABBY proteins of Eucalyptus grandis, Arabidopsis thaliana, and Vitis vinifera were clustered into 5 main groups (FIL, INO, CRC, YAB2 ve YAB5). Segmental duplication was detected between the Egra-YABBY-4/Egra-YABBY-5  genes.  Expression profiles of Egra-YABBY genes have revealed that these genes are expressed in different tissues and can function in a variety of physiological processes during plant development. The results of this study will provide potential biotechnological resources and additional information for further understanding of the molecular bases of the YABBY gene family in the eucalyptus plant.

Keywords

• Domain,in silico analysis,eucalyptus,transcription factor,YABBY

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