The Biological Control of Fusarium oxysporum Causing Wilting in Chickpea (Cicer arietinum L.)

Abstract

This research was conducted to determine the effectiveness of some Trichoderma species against Fusarium oxysporum causing Chickpea wilt disease under in vitro and in vivo conditions between 2017 and 2018. On the experiment conducted in PDA medium by using dual culture technique with an incubation at 22 ± 24^ºC against two isolates of F. oxysporum (N5 and N7), the most effective isolates were T. hamatum ÖT 16, T. asperellum ÖT1, T. strigosum LO43, T. gamsii VG47, and T. gamsii VG48, respectively. In vivo experiment was carried out by applying an antagonist to chickpea seeds at artificially contaminated soil. Trichoderma affected pathogen in different rates. The most effective isolates were T. hamatum ÖT16, T. viride VG18, and T. gamsii VG47, respectively. In the study, it was concluded that Trichoderma is effective in biological control against chickpea wilt disease.

Keywords

• Chickpea,Fusarium oxysporum,Trichoderma sp.,biological control

Nohut (Cicer arietinum L.)’ta Solgunluğa Neden Olan Fusarium oxysporum’un Biyolojik Mücadelesi

Abstract

Bu çalışma, nohut solgunluk hastalığına neden olan Fusarium oxysporum’a karşı bazı Trichoderma türlerinin etkinliğini belirlemek amacıyla 2017-2018 yılları arasında in vivo ve in vitro koşullarında yürütülmüştür. F. oxysporum’un N5 ve N7 izolatına karşı, PDA besiyerinde, ikili kültür yöntemine göre,  22 ± 24 ^oC’de yürütülen denemede, en etkili izolatlar sırasıyla T. hamatum ÖT 16, T. asperellum ÖT1, T. strigosum LO43, T. gamsii VG47 ve T. gamsii VG48 olarak belirlenmiştir. İn vivo koşullarında ise, bulaşık saksı toprağında, nohut tohumlarına antagonist uygulayarak yürütülmüştür. Trichoderma’ların nohut solgunluk patojenlerini farklı oranlarda etkilediği, en etkili izolatların ise sırasıyla T. hamatum ÖT16, T.viride VG18, T. gamsii VG47 olduğu tespit edilmiştir. Çalışmada, nohut solgunluk hastalığına karşı biyolojik mücadelede,  Trichoderma’ların etkili olduğu sonucuna varılmıştır. 

Keywords

• Nohut,Fusarium oxysporum,Trichoderma sp.,biyolojik mücadele

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