Yabani Ravent (Rheum ribes L.)’in Doku Kültürü ile Çoğaltım Olanakları Üzerine Araştırma

Year 2017, Volume: 4 Issue: 3, 296 - 301, 31.10.2017

Burcu Tuncer , Büşra Günsan

https://doi.org/10.19159/tutad.323431

Cited By: 2

Abstract

Rheum ribes L.
tıbbi öneme sahip çok yıllık yabani bir bitki türüdür. Bu çalışmada, in vitro koşullarda büyütülmüş 7 günlük fidelerden
alınan farklı eksplant tipleri (hipokotil ve kotiledon), Indol-3-butirik
asit (IBA) (0.0
ve 1.0 mg L^-1) ve 6-Benzilaminopurin (BAP) (0.0, 1.0 ve 2.0 mg L^-1) kombinasyonları
ilave edilmiş MS (Murashige
and Skoog) besin ortamında kültüre alınmıştır. Araştırma
sonucunda sürgün ve kallus oluşumu yönünden hipokotil eksplantlarının kotiledon
eksplantlarından daha başarılı olduğu belirlenmiştir. Hipokotil eksplantlarında,
eksplant başına en yüksek sürgün sayısı (4.0 adet eksplant^-1) 2mg L^-1 BAP ve 1 mg L^-1
IBA ilavesi yapılmış MS ortamından (MS6) elde edilirken, kallus oluşum oranı
açısından ise MS4 (MS + 1 mg L^-1 BAP + 1 mg L^-1 IBA) (% 88.8)
ve MS6 (MS + 2 mg L^-1 BAP + 1 mg L^-1 IBA) (% 83.3)
ortamlarının daha başarılı olduğu tespit edilmiştir.

Keywords

Rheum ribes L., in vitro rejenerasyon, kallus, sürgün

Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.)

Abstract

Rheum ribes L. is a perennial wild medicinal plant. In this study, the different explants types (hypocotyl and
cotyledon) taken from 7-days-old plantlets were cultured in MS (Murashige and Skoog) media supplemented with 0.0, 1.0
and 2.0 mg L-1 of 6-Benzylaminopurine (BAP) with 0.0 and 1.0 mg L-1 of Indole-3-butyric acid (IBA). Hypocotyl explants
were found to be more successful than cotyledon explants in terms of shoot and callus formation. The highest number of
shoots per explant (4.0 shoot explant-1
) was obtained from MS medium supplemented with 2 mg L-1 BAP and 1 mg L-1 IBA
(MS6) from the hypocotyl explants. In terms of the rate of callus formation (%), MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA)
(88.8%) and MS6 medium (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (83.3%) were found to be more successful than the
hypocotyl explants.

Keywords

Rheum ribes L., in vitro regeneration, callus, shoot

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