Yerel Soğan (Allium cepa L.) Genotiplerinin Haploidiye Yatkınlığının Belirlenmesi

Year 2019, Volume: 6 Issue: 2, 186 - 192, 30.06.2019

Sinan Şahinalp Faika Yaralı Karakan

https://doi.org/10.19159/tutad.523682

Abstract

Bu çalışmada, Türkiye’de
yerel olarak yetiştirilen bazı soğan (Allium
cepa L.) genotiplerinin çiçek tomurcuğu kültürü yoluyla haploidiye
yatkınlıklarının belirlenmesi amaçlanmıştır. Araştırmada kallus oluşumu,
kallusların bitkiye dönüşümü ve eksplantlardan doğrudan bitki gelişimi üzerine genotip
etkisi önemli bulunmuştur. Çalışmada kalluslardan ve doğrudan bitki gelişimi
yoluyla 39 adet bitki elde edilmiştir. Yapılan ploidi analizleri sonucu Şanlıurfa
ili Siverek ilçesinden temin edilen 13 numaralı genotipten elde edilen bir adet
bitkinin haploid olduğu tespit edilmiştir. Dolayısıyla, diğer genotiplerle karşılaştırıldığında, hem
eksplantlarda kallus oluşumu hem de doğrudan bitki meydana getirme kabiliyetinin
yüksek olduğu tespit edilen 12, 13 ve 5 numaralı genotiplerin, soğan ıslah programlarında
haploid bitki elde etmede daha başarılı sonuçlar verebileceği düşünülmektedir. 

Keywords

Soğan, haploidi, gynogenezis, tomurcuk kültürü

Determination of Haploidy Frequencies of the Locally Grown Onion (Allium cepa L.) Genotypes

Abstract

In this study, it was
aimed to determine the haploidy frequencies of the some locally grown onion
(Allium cepa L.) genotypes in Turkey by flower bud culture. The effect of
genotype on callus formation, callus transformation to plants and direct plant
formation was found to be statistically significant. In the experiment, 39
plants were obtained from calluses and direct plant growth from flower buds.
Ploidy analysis revealed that one of these plants, genotype number 13 obtained
from (Siverek/Şanlıurfa province), was haploid. Compared to others, genotypes
12, 13 and 5 were found to have high ability on both callus formation and
direct plant formation. Therefore, it is thought that these genotypes can give
more successful results in haploid plant production in onion breeding studies.

Keywords

Onion, haploidy, gynogenesis, flower bud culture

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