The Effect of Toxic Level Ferrous Nutrition Solution Application on Glutathione Reductase and Ascorbate Peroxidase Activities in Fresh Leaves in Some Rice Varieties

Abstract

The aim of this study is to determine the effect of toxic level iron containing complete nutrient solution application on glutathione reductase and ascorbate peroxidase activities in fresh leaves in some rice varieties. Iron in the form of iron sulphate is added to rice varieties grown in sand culture; Four different treatments were applied: I) 0, II) 45 µM Fe (sufficient Fe), III) 3.50 mM Fe (toxic Fe), IV) 3.50 mM Fe (toxic Fe + sand media with bentonite) as four different treatments. Among the varieties grown at toxic iron level (3.50 mM Fe), the variety with the highest ascorbate peroxidase enzyme activity value in fresh leaves is the Hamzadere variety, whereas the lowest variety is seen to be Edirne rice variety. Among the varieties grown at toxic iron level (3.50 mM Fe), the variety with the highest glutathione reductase enzyme activity value in fresh leaves is Hamzadere variety, whereas the lowest variety is seen to be Biga incisi rice variety.

Keywords

• Iron toxicity
• Glutathione reductase
• Ascorbate peroxidase

Bazı Çeltik Çeşitlerinde Toksik Düzeyde Demir İçerikli Tam Besin Çözeltisi Uygulamasının Taze Yapraklarda Glutatyon Redüktaz ve Askorbat Peroksidaz Aktiviteleri Üzerine Etkisi

Öz

Bu çalışmanın amacı, bazı çeltik çeşitlerinde toksik düzeyde demir içerikli tam besin çözeltisi uygulamasının taze yapraklarda glutatyon redüktaz ve askorbat peroksidaz aktiviteleri üzerine etkisini belirlemektir. Kum kültüründe yetiştirilen çeltik çeşitlerine demir sülfat (FeSO4.7H2O) formunda; I) 0, II) 45 µM Fe (yeterli Fe), III) 3.50 mM Fe (toksik Fe), IV) 3.50 mM Fe (toksik Fe + bentonitli ortam) şeklinde olmak üzere dört farklı muamele uygulanmıştır. Toksik demir düzeyinde (3.50 mM Fe) yetiştirilen çeşitler arasında taze yaprakta askorbat peroksidaz enzim aktivite değeri en yüksek çeşidin Ronaldo çeşidi olduğu; buna karşın, en düşük çeşidin ise Edirne çeltik çeşidi olduğu görülmektedir. Toksik demir düzeyinde yetiştirilen çeşitlerden glutatyon redüktaz aktivitesi en yüksek çeltik çeşidinin Osmancık-97 çeşidi olduğu; buna karşın, en düşük çeşidin ise Biga İncisi olduğu tespit edilmiştir.

Anahtar Kelimeler

• Demir toksisitesi
• Glutatyon redüktaz
• Askorbat peroksidaz

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