Bazı Çeltik Çeşitlerinde Toksik Düzeyde Demir İçerikli Tam Besin Çözeltisi Uygulamasının Taze Yapraklarda Glutatyon Redüktaz ve Askorbat Peroksidaz Aktiviteleri Üzerine Etkisi

Yıl 2021, , 127 - 136, 29.12.2021

Ahmet Korkmaz , Güney Akınoğlu

https://doi.org/10.47137/usufedbid.901151

Öz

Bu çalışmanın amacı, bazı çeltik çeşitlerinde toksik düzeyde demir içerikli tam besin çözeltisi uygulamasının taze yapraklarda glutatyon redüktaz ve askorbat peroksidaz aktiviteleri üzerine etkisini belirlemektir. Kum kültüründe yetiştirilen çeltik çeşitlerine demir sülfat (FeSO4.7H2O) formunda; I) 0, II) 45 µM Fe (yeterli Fe), III) 3.50 mM Fe (toksik Fe), IV) 3.50 mM Fe (toksik Fe + bentonitli ortam) şeklinde olmak üzere dört farklı muamele uygulanmıştır. Toksik demir düzeyinde (3.50 mM Fe) yetiştirilen çeşitler arasında taze yaprakta askorbat peroksidaz enzim aktivite değeri en yüksek çeşidin Ronaldo çeşidi olduğu; buna karşın, en düşük çeşidin ise Edirne çeltik çeşidi olduğu görülmektedir. Toksik demir düzeyinde yetiştirilen çeşitlerden glutatyon redüktaz aktivitesi en yüksek çeltik çeşidinin Osmancık-97 çeşidi olduğu; buna karşın, en düşük çeşidin ise Biga İncisi olduğu tespit edilmiştir.

Anahtar Kelimeler

Demir toksisitesi, Glutatyon redüktaz, Askorbat peroksidaz

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

-

The Effect of Toxic Level Ferrous Nutrition Solution Application on Glutathione Reductase and Ascorbate Peroxidase Activities in Fresh Leaves in Some Rice Varieties

Öz

The aim of this study is to determine the effect of toxic level iron containing complete nutrient solution application on glutathione reductase and ascorbate peroxidase activities in fresh leaves in some rice varieties. Iron in the form of iron sulphate is added to rice varieties grown in sand culture; Four different treatments were applied: I) 0, II) 45 µM Fe (sufficient Fe), III) 3.50 mM Fe (toxic Fe), IV) 3.50 mM Fe (toxic Fe + sand media with bentonite) as four different treatments. Among the varieties grown at toxic iron level (3.50 mM Fe), the variety with the highest ascorbate peroxidase enzyme activity value in fresh leaves is the Hamzadere variety, whereas the lowest variety is seen to be Edirne rice variety. Among the varieties grown at toxic iron level (3.50 mM Fe), the variety with the highest glutathione reductase enzyme activity value in fresh leaves is Hamzadere variety, whereas the lowest variety is seen to be Biga incisi rice variety.

Anahtar Kelimeler

Iron toxicity, Glutathione reductase, Ascorbate peroxidase

Proje Numarası

-

Kaynakça

• Koç E, Üstün AS, Arıcı YK. Biber (Capsicum annuum L.) fidelerinde farklı çinko konsantrasyonlarının total protein, hidrojen peroksit içeriği ve peroksidaz aktivitesi üzerine etkisi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 2012;13(2):205-212.
• Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 2010;48:909-930.
• Burzynski M, Klobus G. Changes of photosynthetic parameters in cucumber leaves under Cu, Cd and Pb stress. Photosynth 2004;42(4): 505-510.
• Chen L, Ding C, Zhao X, Xu J, Mohammad AA, Wang S, Ding Y. Differential regulation of proteins in rice (Oryza sativa L.) under iron deficiency. Plant Cell Reports, 2015;34:83-96.
• Becana M, Moran JF, Iturbe-Ormaetxe, I, Escuredo, PR. Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant Soil, 1998;201(1):137-147. doi: 10.1023/A:1004375732137
• Iturbe-Ormaetxe I, Moran JF, Arrese-Igor C, Gogorcena Y, Klucas YC, Becana M. Activated oxygen and antioxidant defences in iron-deficient pea plants. Plant Cell and Environment, 1995;18:421-429.
• Sun B, Jing Y, Chen K, Song L, Chen F, Zhang L. Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize (Zea mays). Journal of Plant Physiology, 2007;164(5):536-43. doi: 10.1016/j.jplph.2006.02.011.
• Daşgan HY, Öztürk L, Abak K, Çakmak I. Activities of iron-containing enzymes in leaves of two tomato genotypes differing in their resistance to Fe chlorosis. Journal of Plant Nutrition, 2003;26:1997-2007.
• Majerus V, Bertin P, Swenden V, Fortemps A, Lobréaux S, Lutts S. Organ-dependent responses of the African rice to short-term iron toxicity: ferritin regulation and antioxidative responses. Biology Plant, 2007b;51:303-312.
• Saikia T, Baruah, KK. Iron Toxicity Tolerance in Rice (Oryza sativa) and Its Association with Anti-Oxidative Enzyme Activity. Journal of Crop Science, 2012;3(3):90-94.
• Smirnoff N. Ascorbate, tocopherol and carotenoids: metabolism, pathway engineering and functions. In: Smirnoff N (Ed) Antioxidants and reactive oxygen species in plants. 1st Edn. Oxford: Blackwell Publishing Ltd, 2005. p. 53-86.
• Tiryakioglu M, Eker S, Özkutlu F, Husted S, Çakmak I. Antioxidant defense system and cadmium uptake in barley genotypes differing in cadmium tolerance. Journal of Trace Elements in Medicine and Biology, 2006;20:181-189. doi:10.1016/j.jtemb.2005.12.004
• Zhang X, Zhang F, Mao D. Effect of Fe plaque outside roots on nutrient uptake by rice (Oryza sativa L.): zinc uptake. Plant and Soil, 1998;202:33-39.
• Amako K, Chen G-X, Asada K. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolicisozymes of ascorbate peroxidase in plants. Plant Cell Physiology, 1994;35:497-504.
• Jiang M, Zhang J. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. Journal of Experimental Botany, 2002;53(379):2401-2410.
• Peter H. Protoctor and Edward S.Reynolds, Free radicals and disaase in man, Psiolo Che-Phys-Med, 1984;16:175-195.
• Chou T-S, Chao Y-Y, Huang W-D, Hong C-Y. Effect of magnesium deficiency on antioxidant status and cadmium toxicity in rice seedlings. Journal of Plant Physiology, 2011;168(10):1021-1030.
• Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 2002;7(9): 405-410.
• Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G. Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environmental and Experimental Botany, 2006a;56(1):54-62.
• Corpas FJ, Palma JM, Sandalio LM, López-Huertas E, MC, Romero-Puertas MC, Barroso JB, Del Río LA. Purification of catalase from pea leaf peroxisomes: identification of five different isoforms. Free Radical Research, 1999;31:235-241.