Öz
The extents of glycosylation of nail
and hair proteins ıvere determined by the colorimetric thiobarbituric acid
method in 15 experimentally alloxan induced diabetic and 15 nondiabetic Albino rabbits.
Blood was taken from both diabetics and nondiabetics for measurement of
hemoglobin glycosylation at the same time. In nondiabetics, the protein glycosylation
in nail, hair and glycosylated hemoglobin were found 1.5 ± 0.37 nmol
fructosamine per mg nail, 0,61 ± 0.06 nmol fructosamine per mg hair and 1.7 ± 0.49
nmol fructosamine per mg Hb. In the diabetic group there ıvas an extremely high
glycosylation in nail, hair proteins and hemoglobin: 3.2 ± 0.88 nmol
fructosamine per mg nail, 0.69 ± 0.007 nmol fructosamine per mg hair and 3.15 ±
0.98 nmol fructosamine per mg Hb.
Glycosylation of nail, hair and hemoglobin
might provide a stable long term measure of tissue glycosylation, useful in the
investigation of diabetic microvascular complications.
Anahtar Kelimeler
Kaynakça
- Jovanovic L and Peterson CM : The clinical utility of glycosylated hemoglobin. J Am Med. 70: 331, 1981.
- Mc Donalt JM and Davis JR : Glycosylated hemoglobins and diabetes mellitus. Human Pathol. 10 : 279, 1981.
- Goner B and Rubenstein AH : Hemoglobin Aj and diabetes mellitus. Diabetologia. 15 : 1, 1978.
- Bunn HF, Gabbay KH and Gallop PM : The glycosy- lation of Hb : Relevance to diabetes mellitus. Science. 200 : 21, 1978.
- Seven A : Increased glycation of hair and nail proteins in patients with diabetes. Türk J Med Biol Res. 4 : 140, 1993.
- Bakan E, Bakan N ve Ağbaş A : Diabetli hastalarda tırnak proteinlerinin glikozilasyonu. C 10 : 8, 1986.
- Sueki H : Glycosylated proteins of skin, nail and hair : Application as an mdex for long-term control of diabetes mellitus. J Dermatol. 16 : 103, 1989.
- Seven A : Correlation between dıabetic neuropathy and glycated hemoglobin, hairglycation and blood sorbitol. MedSciRes. 21 : 161, 1993.
- Seven A and Candan G : Advanced glycation endpro- ducts and diabetes mellitus. Türk J Med Biol Res. 3 : 225, 1992.
- Rakhimova GN, Akbarov ZS and Turakulov IaKII : Determining the time of diabetes mellitus onset by the level of glycosylated keratin in hair. Probl Endokrinol. 38 (3): 21, 1992.
- Parker KM, England JD and Da Cosfta J : Improved colorimetric assay for glycosylated hemoglobin. Clin Chem. 27:669,1981.
- Oimomi M : Increased fructose-Lysine of nail protein in diabetic patients. Klin Vvochenschr. 62 (10): 477, 1984.
- PaiseyRB, Clamp JR and Kent MJC : Glycosylatıon of hair : Possible marker of chronic hvperglycemia. British MedJ. 288: 669, 1984.
- Oimomi M, Nishimotoa S and Kitamura Y : Increased fructose-lysine of nail protein and blood glucose control ııı diabetic patients. Horm Metab Res 18: 827, 1986.
- Oimomi M and Igaki N : Hair protein glycation as a long-term. index of blood glucose in diabetics. Diabetes Res and Clin Pract. 5 : 305, 1988.
Öz
Kıl
ve tırnak proteinlerinde hiperglisemiye bağlı olarak meydana gelen glikozillenmeyi
ölçmek için 15 normal ve alloksan ile diabet oluşturulmuş 15 Albino tavşandan
tırnak ve kıl örnekleri alındı. Glikozilasyon, kolorimetrik tiyobarbitürik asit
metoduyla ölçüldü. Tırnak ve kıl örnekleri alındığında hemoglobin
glikozilasyonunu ölçmek için her iki gruptan kan örnekleri alındı. Normal
tavşanlarda glikozillenmiş hemoglobin 1.7 ± 0.49 nanomol früktozamin / mg Hb, tırnaktaki
protein glikozilasyonu 1.5 ± 0.37 nanomol früktozamin / mg tırnak ve
glikozillenmiş kıl protein glikozilasyonu 0.61 ± 0.06 nanomol früktozamin / mg
kıl bulundu. Diyabetli tavşanlarda bu değerler daha yüksek olup : sırasıyla
3.15 ± 0.98 nanomol früktozamin / mg Hb, 3.2 ± 0.88 nanomol früktozamin / mg tırnak
ve 0.69 ± 0.007 nanomol früktozamin / mg kıl olarak tespit edildi.
Bu çalışmadaki bulgular, doku glikozilasyonunun uzun
süreli stabil bir göstergesi olarak tırnak ve kıl proteinlerinin
glikozilasyonunu belirlemenin diabetin mikrovasküler komplikasyonlarının araştırılmasında
yararlı olacağını göstermektedir.
Anahtar Kelimeler
Kaynakça
- Jovanovic L and Peterson CM : The clinical utility of glycosylated hemoglobin. J Am Med. 70: 331, 1981.
- Mc Donalt JM and Davis JR : Glycosylated hemoglobins and diabetes mellitus. Human Pathol. 10 : 279, 1981.
- Goner B and Rubenstein AH : Hemoglobin Aj and diabetes mellitus. Diabetologia. 15 : 1, 1978.
- Bunn HF, Gabbay KH and Gallop PM : The glycosy- lation of Hb : Relevance to diabetes mellitus. Science. 200 : 21, 1978.
- Seven A : Increased glycation of hair and nail proteins in patients with diabetes. Türk J Med Biol Res. 4 : 140, 1993.
- Bakan E, Bakan N ve Ağbaş A : Diabetli hastalarda tırnak proteinlerinin glikozilasyonu. C 10 : 8, 1986.
- Sueki H : Glycosylated proteins of skin, nail and hair : Application as an mdex for long-term control of diabetes mellitus. J Dermatol. 16 : 103, 1989.
- Seven A : Correlation between dıabetic neuropathy and glycated hemoglobin, hairglycation and blood sorbitol. MedSciRes. 21 : 161, 1993.
- Seven A and Candan G : Advanced glycation endpro- ducts and diabetes mellitus. Türk J Med Biol Res. 3 : 225, 1992.
- Rakhimova GN, Akbarov ZS and Turakulov IaKII : Determining the time of diabetes mellitus onset by the level of glycosylated keratin in hair. Probl Endokrinol. 38 (3): 21, 1992.
- Parker KM, England JD and Da Cosfta J : Improved colorimetric assay for glycosylated hemoglobin. Clin Chem. 27:669,1981.
- Oimomi M : Increased fructose-Lysine of nail protein in diabetic patients. Klin Vvochenschr. 62 (10): 477, 1984.
- PaiseyRB, Clamp JR and Kent MJC : Glycosylatıon of hair : Possible marker of chronic hvperglycemia. British MedJ. 288: 669, 1984.
- Oimomi M, Nishimotoa S and Kitamura Y : Increased fructose-lysine of nail protein and blood glucose control ııı diabetic patients. Horm Metab Res 18: 827, 1986.
- Oimomi M and Igaki N : Hair protein glycation as a long-term. index of blood glucose in diabetics. Diabetes Res and Clin Pract. 5 : 305, 1988.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Orijinal Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 6 Haziran 1995 |
| Gönderilme Tarihi | 21 Şubat 1995 |
| Yayımlandığı Sayı | Yıl 1995 Cilt: 1 Sayı: 1 |
Van Health Sciences Journal (Van Sağlık Bilimleri Dergisi) başlıklı eser bu Creative Commons Atıf-Gayri Ticari 4.0 Uluslararası Lisansı ile lisanslanmıştır.
