The Effect of a Highly Saturated Fat Diet and Intermittent Fasting Diet On Experimental Colon Cancer Development And Some Serum Inflammation Markers in Rats Intermittent Fasting And Inflammation

Year 2024, Volume: 17 Issue: 1, 15 - 24, 30.04.2024

Nizamettin Günbatar , Fahri Bayiroglu , Hüseyin Emlik

https://doi.org/10.52976/vansaglik.1321114

Abstract

Objective: In this study, the effects of complete food restriction (modified intermittent feeding) on serum inflammation markers and cancer development in rats supplemented with a highly saturated fat diet fed and exposed to colonotropic carcinogenic substances were investigated. Materials and Methods: Wistar albino rats were divided into two groups as 15 rats in each group. After two weeks of high-fat diet pre-feeding in both groups, high-fat feeding was continued for 12 weeks. The trial group was kept completely free of feed only 2 days a week. Dimethylhydrazine (DMH), a colonotropic chemical carcinogen, was administered in two doses of 25 and 125 mg / kg subcutaneously at the beginning of the trial. Results: At the end of the study, serum Crp, Tnf-α and Il-6, Il-1 β, Insulin, Leptin and Igf-1 parameters were measured. Serum crp, Tnf-α (p <0.01), Il-6 (p <0.05) The difference between IL-1 β, Insulin, Leptin and Igf-1 values was not statistically significant. Conclusion:Although the dietary diet continues, it is concluded that this study can prevent obesity and systemic inflammation which is the condition of many degenerative diseases

Keywords

Rat, Serum, Cancer, Hıgh Fat Diet, Obesity

Project Number

2013-SBE-D074

Sıçanlarda Yüksek Oranda Doymuş Yağlı Diyet ile Aralıklı Beslemenin Deneysel Kolon Kanseri Gelişimi ve Bazı Serum İnflamasyon Markırları Üzerine Etkisi

Abstract

Amaç: Bu çalışmada yüksek oranda yağlı diyetle beslenen ve kolonotropik kanserojen maddeye maruz bırakılan ratlarda, haftada sadece 2 gün tam gıda kısıtlamasının (modifiye aralıklı besleme) serum inflamasyon markırları ve kanser gelişimi üzerine etkileri incelendi. Materyal ve Metod: Wistar albino ratlar her iki grupta 15 rat olacak şekilde deney (aralıklı besleme) ve kontrol olmak üzere iki gruba ayrıldı. Her iki grubtada yüksek yağlı diyetle 2 hafta ön besleme sonrasında, 12 hafta boyunca yüksek yağlı beslemeye devam edildi. Deneme grubu haftada sadece 2 gün tamamen yemden uzak tutuldu. Her iki gruba da deneme başında kolonotropik kimyasal karsinojen olan Dimetilhidrazin (DMH) 2 doz halinde 25 ve 125 mg/kg subkutan enjeksiyon şeklinde yapıldı. Bulgular: Çalışma sonunda serumda Crp, Tnf- α ve Il-6, Il-1 β, İnsülin, Leptin ve İgf-1 parametrelerin bakıldı. Deney grubunda serum crp, Tnf- α (p<0.01), Il-6 (p<0.05) öneminde düşük bulunurken, Il-1 β, İnsülin, Leptin ve İgf-1 değerleri arasındaki fark istatistiki önemde görülmedi. Sonuç: Modifiye edilen aralıklı besleme uygulamasının yüksek yağlı diyet beslenmesine devam edilmesine rağmen, obesiteyi ve birçok dejeneratif hastalığın hazırlayıcısı olan sistemik inflamasyonu önleyebileceği kanaatine varıldı.

Keywords

Rat, Serum, Kanser, Yüksek Yağlı Diet, Obezite

Supporting Institution

Bu araştırma Doktora Tezinden özetlenmiş olup, Yüzüncü Yıl Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Project Number

2013-SBE-D074

Thanks

Bu araştırma Doktora Tezinden özetlenmiş olup, Yüzüncü Yıl Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2013-SBE-D074 nolu proje ile desteklenmiştir.

References

• Allen TL, Febbraio MA. (2010). IL6 as a mediator of insulin resistance: fat or fiction? Diabetologia. 53, 399–402.
• Anson RM, Guo Z, Cabo R, Iyun T, Rios M, Hagepanos A. (2003). Intermittent fasting dissociates beneficial effects of dietary restriction on glucose metabolism and neuronal resistance to injury from calorie intake. Proceedings of the National Academy of Sciences of the United States of America, 100, 6216–6220.
• Bishop DJ, Botella J, Genders AJ, Lee M J, Saner N J, Kuang J, Yan X, Granata C. (2018). High-ıntensity exercise and mitochondrial biogenesis: current controversies and future research directions. Physiology, 34,56–70.
• Cani PD, Bibiloni C, Knauf A, Waget AM. (2008). Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice, Diabetes, 57, 1470–1481.
• Cissé YM, Borniger JC, Lemanski E, Walker W H, 2nd; Nelson R J. (2018). Time-restricted feeding alters the ınnate ımmune response to bacterial endotoxin. Journal of Immunology, 200,681–687.
• Dunn SE, Kari FW, French J, Leininger JR, Travlos G, Wilson R, B et al. (1997). Dietary restriction reduces insulin-like growth factor I levels, which modulates apoptosis, cell proliferation, and tumor progression in p53-deficient mice. Cancer Research, 57, 4667–4672.
• Faloia EG, Michetti M, de Robertis MP, Luconi G, Furlani MB. (2012). Inflammation as a link between obesity and metabolic syndrome. Journal of Nutrition and Metabolism, doi:10.1155/2012/476380
• Faraj M, Havel PJ, Phelis S, Blank D, Sniderman AD, Cianflone K. (2003). Plasma acylation-stimulating protein, adiponectin, leptin and ghrelin before and after weight loss induced by gastric bypass surgery in morbidly obese subjects. The Journal of Clinical Endocrinolocy and Metabolism, 88,1594-1602.
• Faris MA, Kacimi S, Al-Kurd RA, Fararjeh MA, Bustanji YK, Mohammad MK, et al. (2012). Intermittent fasting during Ramadan attenuates proinflammatory cytokines and immune cells in healthy subjects. Nutrition Research, 32, (12), 947-955.
• Fontana L, Klein S. (2007). Aging, adiposity, and calorie restriction. The Journal of the American Medical Association, 297, 986–994.
• Fresno M, Alvarez R, Cuesta N. (2011). Toll-like receptors, inflammation, metabolism and obesity. Archievs of Physiology and Biochemistry, 117,151–164.
• Garcia MC, Wernstedt I, Berndtsson A, Enge M, Bell M, Hultgren O, et al. (2006). Mature-onset obesity in interleukin-1 receptor I knockout mice. Diabetes, 55,1205-1213.
• Gregor MF, Hotamisligil GS. (2011). Inflammatory mechanisms in obesity. Annual Review of Immunology, 29, 415-445.
• Hernandez H, Simental-Mendía LE,Rodríguez-Ramírez G, Reyes-Romero MA. (2013). Obesity and inflammation: epidemiology, risk factors, and markers of inflammation. International Journal of Endocrinology, 2013, 678159.
• Kern PA, Subramanian R, Chunling LI, Linda W, Gouri R. (2001). Adipose tissue tumor necrosis factor and interlleukin-6 expression in human obesity and insulin resistance. The American Journal of Physiology–Endocrinology and Metabolism, 280, 745-751.
• Klempel MC, Kroeger CM, Bhutani S, Trepanowski JF, Varady KA. (2012). Intermittent fasting combined with calorie restriction is effective for weight loss and cardio protection in obese women. Nutrition, J11, 98-104.
• Matsuki T, Horai R, Sudo K, Iwakura Y (2003).IL-1 plays an important role in lipid metabolism byregulating insulin levels under physiological conditions. Jornal of Experimental Medicine, 198, 877–888.
• Mito N, Yoshino H, Hosoda T, Sato K. (2004). Analysis of the effect of leptin on immune function in vivo using diet- induced obese mice. Jornal of Endocrinology, 180(1), 167-173.
• Mohamad-Shahi M, Karandish M, Haidari F, Omidian K, Fatemi-Tabatabayei SR, Rafiei H. (2012). Effect of daidzein-low-calorie diet on body weight, serum levels of glucose, resistin, and high sensitive C-reactive protein in high fat, high calorie diet induced rats. Saudi Medical Journal, 33, 70-75.
• Mosby TT, Cosgrove M, Sarkardei S, Platt KL, Kaina B. (2012). Nutrition in adult and childhood cancer: role of carcinogens and anti-carcinogens. Anticancer Research, 32, (10), 4171-4192.
• Murphy N, Jenab M, Gunter MJ. (2018). Adiposity and gastrointestinal cancers: epidemiology, mechanisms and future directions. Nature Reviews Gastroenteroloy and Hepatology, 15, 659–670.
• Nov O, Shapiro H, Ovadia H, Tarnovscki T, Dvir I, Shemesh E, et al.(2013). Interleukin-1β regulates fat-liver crosstalk in obesity by auto-paracrine modulation of adipose tissue inflammation and expandability, PloS One, 8,1.
• Park S, Park NY, Valacchi G, Lim Y. (2012). Calorie restriction with a high-fat diet effectively attenuated inflammatory response and oxidative stress-related markers in obese tissues of the high diet fed rats. Mediators of Inflammation, doi: 1155/2012/984643
• Rahbar AR, Safavi E, Rooholamini M, Jaafari F, Darvishi S, Rahbar A. (2019). Effects of ıntermittent fasting during ramadan on ınsulin-like growth factor-1, ınterleukin 2, and lipid profile in healthy muslims. International Journal of Preventive Medicine, 15(10),7.
• Sandhu MS, Dunger DB, Giovannucci EL. (2002). Insulin, insulin-like growth factor-I (IGF-I), IGF binding proteins, their biologic interactions, and colorectal cancer. Journal of National Cancer Institute, 94, 972–980.
• Soner D, Amitabha R, Margot PC. (2017). The influence of different calorie restriction protocols on serum pro-inflammatory cytokines, adipokines and IGF-I levels in female C57BL6 mice: Short term and long term diet effects. Meta Gene, 12.22-32.
• Su LJ, Mahabir GL, Ellison LA, McGuinn BCR. (2012). Epigenetic contributions to the relationship between cancer and dietary intake of nutrients, bioactive food, components, and environmental toxicants. Nutrigenomics, 2, 1–12.
• Sproston NR, Ashworth JJ. (2018). Role of C-reactive protein at sites of inflammation and infection. Frontier Immunology, 9,1–11.
• Syrenicz A, Garanty-Bogacka B, Syrenicz M, Gebala A, Walczak M. (2006). Lowgrade systemic inflammation and the risk of type 2 diabetes in obese children and adolescents. Neuroendocrinology Letter, 27(4), 453–458.
• Varady KA, Bhutani S, Klempel MC. (2013). Alternate day fasting for weight loss in normal weight and overweight subjects: a randomized controlled trial. Nutriton Journal, 12,146.
• Vasconcelos AR, Yshii LM, Viel TA, Buck HS, Mattson MP. (2014). Intermittent fasting attenuates lipopolysaccharide induced neuroinflammation and memory impairment. Journal of Neuroscience, 11, 85.
• Versini M, Jeandel PY, Rosenthal E, Shoenfeld Y. (2014). Obesity in autoimmune diseases: Not a passive bystander. Autoimmunity. Reviews, 13, 981–1000. Wan R, Ahmet I, Brown M, Chen GA, Kamimura N, Talan M, et al. (2010). Cardioprotective effect of intermittent fasting is associated with an elevation of adiponectin levels in rats. Journal of Nutritional Biochemistry, 21(5), 413-417.