The Effect of a Highly Saturated Fat Diet and Intermittent Fasting Diet On Experimental Colon Cancer Development And Some Serum Inflammation Markers in Rats Intermittent Fasting And Inflammation

Yıl 2024, Cilt: 17 Sayı: 1, 15 - 24, 30.04.2024

Nizamettin Günbatar , Fahri Bayiroglu , Hüseyin Emlik

https://doi.org/10.52976/vansaglik.1321114

Öz

Objective: In this study, the effects of complete food restriction (modified intermittent feeding) on serum inflammation markers and cancer development in rats supplemented with a highly saturated fat diet fed and exposed to colonotropic carcinogenic substances were investigated. Materials and Methods: Wistar albino rats were divided into two groups as 15 rats in each group. After two weeks of high-fat diet pre-feeding in both groups, high-fat feeding was continued for 12 weeks. The trial group was kept completely free of feed only 2 days a week. Dimethylhydrazine (DMH), a colonotropic chemical carcinogen, was administered in two doses of 25 and 125 mg / kg subcutaneously at the beginning of the trial. Results: At the end of the study, serum Crp, Tnf-α and Il-6, Il-1 β, Insulin, Leptin and Igf-1 parameters were measured. Serum crp, Tnf-α (p <0.01), Il-6 (p <0.05) The difference between IL-1 β, Insulin, Leptin and Igf-1 values was not statistically significant. Conclusion:Although the dietary diet continues, it is concluded that this study can prevent obesity and systemic inflammation which is the condition of many degenerative diseases

Anahtar Kelimeler

Rat, Serum, Cancer, Hıgh Fat Diet, Obesity

Proje Numarası

2013-SBE-D074

Sıçanlarda Yüksek Oranda Doymuş Yağlı Diyet ile Aralıklı Beslemenin Deneysel Kolon Kanseri Gelişimi ve Bazı Serum İnflamasyon Markırları Üzerine Etkisi

Öz

Amaç: Bu çalışmada yüksek oranda yağlı diyetle beslenen ve kolonotropik kanserojen maddeye maruz bırakılan ratlarda, haftada sadece 2 gün tam gıda kısıtlamasının (modifiye aralıklı besleme) serum inflamasyon markırları ve kanser gelişimi üzerine etkileri incelendi. Materyal ve Metod: Wistar albino ratlar her iki grupta 15 rat olacak şekilde deney (aralıklı besleme) ve kontrol olmak üzere iki gruba ayrıldı. Her iki grubtada yüksek yağlı diyetle 2 hafta ön besleme sonrasında, 12 hafta boyunca yüksek yağlı beslemeye devam edildi. Deneme grubu haftada sadece 2 gün tamamen yemden uzak tutuldu. Her iki gruba da deneme başında kolonotropik kimyasal karsinojen olan Dimetilhidrazin (DMH) 2 doz halinde 25 ve 125 mg/kg subkutan enjeksiyon şeklinde yapıldı. Bulgular: Çalışma sonunda serumda Crp, Tnf- α ve Il-6, Il-1 β, İnsülin, Leptin ve İgf-1 parametrelerin bakıldı. Deney grubunda serum crp, Tnf- α (p<0.01), Il-6 (p<0.05) öneminde düşük bulunurken, Il-1 β, İnsülin, Leptin ve İgf-1 değerleri arasındaki fark istatistiki önemde görülmedi. Sonuç: Modifiye edilen aralıklı besleme uygulamasının yüksek yağlı diyet beslenmesine devam edilmesine rağmen, obesiteyi ve birçok dejeneratif hastalığın hazırlayıcısı olan sistemik inflamasyonu önleyebileceği kanaatine varıldı.

Anahtar Kelimeler

Rat, Serum, Kanser, Yüksek Yağlı Diet, Obezite

Destekleyen Kurum

Bu araştırma Doktora Tezinden özetlenmiş olup, Yüzüncü Yıl Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Proje Numarası

2013-SBE-D074

Teşekkür

Bu araştırma Doktora Tezinden özetlenmiş olup, Yüzüncü Yıl Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2013-SBE-D074 nolu proje ile desteklenmiştir.

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