Investigation of the Effects of Chrysin Against Amoxycillin-Clavulanic Acid-Induced Kidney Injury in Rats

Abstract

The amoxycillin-clavulanic acid (ACA) used in the treatment of various bacterial infections often causes drug-induced tissue damage, but the mechanism of this damage has not yet been fully elucidated. Chrysin (CHR) is a natural flavonoid with various pharmacological properties as well as antioxidant and anti-inflammatory properties. In this study, the protective effect of CHR against ACA-induced kidney damage, which is frequently used in human and animal health, was investigated. Twenty-eight female rats were divided into four groups as control, CHR, ACA and ACA+CHR. ACA (30 mg/kg) and CHR (50 mg/kg) were administered orally once a day for seven days. Renal function, oxidative stress and inflammation parameters were analyzed to determine renal tissue damage. Histopathologic analysis was also performed to detect tissue damage and structural changes. According to the data obtained from these analyses, ACA increased urea and creatinine levels in kidney tissue. ACA administration also increased malondialdehyde (MDA) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) activities and glutathione (GSH). Nuclear factor kappa B (NF-B), tumor necrosis factor-alpha (TNF-α) and interleukin 1β (IL-1β) expression levels were found to increase. Administration of CHR together with ACA decreased urea, creatinine, MDA, NF-B, TNF-α, IL-1β levels and increased GSH level and GPx, SOD, CAT activities. When the findings were evaluated together, it was determined that ACA caused renal damage by increasing renal function levels, oxidative stress and inflammation, while supportive treatment of CHR reduced renal damage by bringing these parameters closer to normal.

Keywords

• Amoxycillin-Clavulanic Acid
• Chrysin
• Oxidative stress
• Inflammation
• Kidney

Sıçanlarda Amoksisilin/Klavulanik Asit ile Oluşturulan Böbrek Hasarına Karşı Krisin’in Etkilerinin Araştırılması

Abstract

Çeşitli bakteriyel enfeksiyonların tedavisinde kullanılan amoksisilin-klavulanik asit (ACA), çoğunlukla ilaç kaynaklı doku hasarına neden olmaktadır fakat bu hasarın mekanizması henüz tam olarak aydınlatılmamıştır. Krisin (CHR), antioksidan ve antiinflamatuar gibi özelliklerinin yanı sıra çeşitli farmakolojik özellikleri bulunan doğal bir flavonoiddir. Bu çalışmada, insan ve hayvan sağlığında sıklıkla kullanılan ACA’nın neden olduğu böbrek hasarına karşı CHR’nin koruyucu etkisi araştırıldı. Yirmi sekiz adet dişi sıçan kontrol, CHR, ACA ve ACA+CHR olmak üzere dört grup ayrıldı. ACA (30 mg/kg) ve CHR (50 mg/kg) yedi gün boyunca günde bir kez oral yoldan uygulandı. Böbrek dokusunda hasarı belirlemek için böbrek fonksiyonu, oksidatif stres ve inflamasyon parametreleri analiz edildi. Doku hasarı ve yapısal değişiklikleri tespit etmek için histopatolojik analiz yapıldı. Bu analizler sonucunda elde edilen verilere göre ACA, böbrek dokusunda üre ve kreatin düzeylerini artırdı. ACA uygulamasının aynı zamanda malondialdehit (MDA)’i arttırdığı, glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD), katalaz (CAT) aktivitelerini ile glutatyon (GSH)’u azalttığı bulundu. Nuclear factor kappa B (NF-B), tumor necrosis factor-alpha (TNF-α) ve interleukin 1β (IL-1β) ekspresyon düzeylerini artırdığı tespit edildi. CHR’nin ACA ile birlikte uygulanmasının üre, kreatin, MDA, NF-B, TNF-α, IL-1β düzeylerini azalttığı, GSH düzeyi ile GPx, SOD, CAT aktivitelerini artırdığı belirlendi. Elde edilen bulgular birlikte değerlendirildiğinde, ACA’nın böbrek fonksiyon düzeylerini, oksidatif stresi ve inflamasyonu artırarak böbrek hasarına neden olduğu, CHR’nin destekleyici tedavisinin ise bu parametreleri normale yakınlaştırarak böbrekte hasarı azalttığı tespit edildi.

Keywords

• Amoksisilin/Klavulanik Asit
• Krisin
• Oksidatif stres
• İnflamasyon
• Böbrek.

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