PCR detection of Anaplasma phagocytophilum in stray dogs in Batman, Turkey

Year 2019, Volume: 30 Issue: 3, 183 - 185, 22.11.2019

Bekir Oğuz Serdar Deger

https://doi.org/10.36483/vanvetj.621542

Cited By: 2

Abstract

Anaplasma phagocytophilum causes
high-grade fever in humans, ruminants, horses, cats, and dogs. A. phagocytophilum, which causes
granulocytic anaplasmosis in dogs, has a wide geographical distribution in the
world, but there are not many studies on the Anaplasma species in dogs in
Turkey. A.
phagocytophilum, which infects leukocytes in dog, might also be
zoonotic. This study aimed to investigate the A. phagocytophilum prevalence in stray dogs in Batman province of
Turkey using Polymerase Chain Reaction (PCR) technique. Blood samples were
drawn into EDTA tubes from the venae cephalica antebrachii of randomly selected
97 asymptomatic dogs in total. Identification of A. phagocytophilum was carried out using the conventional PCR
method that was conducted by using the DNAs obtained from the samples. A. phagocytophilum prevalence was found
to be 3.1% in the examined blood samples. In conclusion, infection of dogs in
Batman province with A. phagocytophilum
has been revealed molecularly for the first time with this study. It is considered
that detection of this species, which can cause disease (Human granulocytic
anaplasmosis) in humans as well, has contributed epidemiologically to the data
on the spread of the disease throughout Turkey. Moreover, investigating the tick
species that transmit the disease in the region where the study was conducted
is important for developing effective control strategies against the disease.

Keywords

Anaplasma phagocytophilum, Dog, Pathogen, PCR

Thanks

We would like to thank Veterinarian Onur Kilic for their assistance in sample collection.

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