Ratlarda Siklofosfamid ile Deneysel Olarak Oluşturulan Anemi Modelinde Smilax excelsa L. Etanol Ekstresinin Etkisi

Year 2021, Volume: 32 Issue: 3, 114 - 118, 26.11.2021

Mustafa Cellat , Tuba Aydın

https://doi.org/10.36483/vanvetj.972147

Abstract

Bu çalışmada, ratlarda siklofosfamid (CP) ile deneysel olarak oluşturulan anemi modelinde Smilax excelsa L. etanol ekstresinin etkisi araştırıldı. Çalışma toplam 4 gruptan oluştu. Grup 1 ve 2’deki ratlara oral gavaj yöntemi kullanılarak 28 gün süre ile günlük 1 ml serum fizyolojik, grup 3 ve 4’deki ratlara ise 400 mg/kg dozda Smilax excelsa L. etanol ekstresi uygulandı. Ayrıca grup 2 ve 4’deki ratlara haftada bir kez olmak üzere toplam dört doz 50 mg/kg CP uygulaması intramusküler olarak yapıldı. Çalışmanın 28. günü bütün ratlardan anestezi altında kan örnekleri alındı ve daha sonra ötanazi işlemi uygulandı. Kan örneklerinde eritrosit sayısı (RBC), lökosit sayısı (WBC), hemoglobin konsantrasyonu (HGB), hematokrit değer (Hct), ortalama eritrosit hemoglobini (MCH), ortalama eritrosit hacmi (MCV) ve ortalama eritrosit dağılım genişliği (RDW) gibi hematolojik parametreler incelendi. Serum malondialdehit (MDA) ve indirgenmiş glutatyon (GSH) düzeyleri ile katalaz (CAT) ve glutatyonperoksidaz (GSH-Px) enzim aktivitelerine spektrofotometrik olarak bakıldı. Grup 4’de Smilax excelsa L. etanol ekstresi tedavisinin serum MDA düzeyini düşürdüğü, CAT ve GSH-Px enzim aktivitelerini ise arttırdığı belirlendi. Ayrıca bu tedavinin RBC, HGB ve Hct gibi hematolojik parametrelerde iyileşmelere sebep olduğu, RDW değerlerini ise azalttığı gözlemlendi. Sonuç olarak Smilax excelsa’nın sahip olduğu güçlü antioksidan etki ile CP’nin kemik iliğindeki baskılayıcı etkisini azalttığı ve anemi şekillenmesini önlediği görüldü.

Keywords

Aplastik Anemi, Siklofosfamid, Oksidatif Stres, Smilasagiller

The Effect of Smilax excelsa L. Ethanol Extract in an Experimentally Anemia Model İnduced by Cyclophosphamide in Rats

Abstract

In this study, the effect of Smilax excelsa L. ethanol extract on anemia model experimentally induced by Cyclophosphamide (CP) in rats was investigated. The study consisted of 4 groups, The rats in groups 1 and 2 were administered 1 ml of physiological saline for 28 days using the oral gavage method, and the rats in groups 3 and 4 were administered Smilax excelsa L. ethanol extract at a dose of 400 mg/kg. In addition, four doses of 50 mg/kg CP were administered intramuscularly once a week to the rats in groups 2 and 4. On the 28th day of the experiment, blood samples were taken from all rats under anesthesia and then euthanasia was performed. Hematological parameters such as red blood cell count (RBC), leukocyte count (WBC), hemoglobin concentration (HGB), hematocrit value (Hct), mean erythrocyte hemoglobin (MCH), mean erythrocyte volume (MCV), and mean erythrocyte distribution width (RDW) were examined in blood samples. Serum malondialdehyde (MDA) and reduced glutathione (GSH) levels and catalase (CAT) and glutathione peroxidase (GSH-Px) enzyme activities were measured spectrophotometrically. In group 4, it was determined that Smilax excelsa L. ethanol extract treatment decreased the serum MDA level and increased the CAT and GSH-Px enzyme activities. In addition, it was observed that this treatment caused improvements in hematological parameters such as RBC, HGB and Hct, and decreased RDW values. As a result, it was observed that Smilax excelsa, with its strong antioxidant effect, reduced the suppressive effect of CP in the bone marrow and prevented the formation of anemia.

Keywords

Aplastic Anemia, Cyclophosphamide, Oxidative Stress, Smilacaceae

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