Çöven Ekstraktı, Tahin, Saponin ve Sesaminin Tip 2 Diyabetik Farelerde Bazı Biyokimyasal Parametreler Üzerine Etkisi

Year 2024, Volume: 35 Issue: 2, 120 - 124, 31.07.2024

Tuğba Melike Parlak , Bünyamin Traş

https://doi.org/10.36483/vanvetj.1481278

Abstract

Çalışmanın amacı farelerde deneysel olarak oluşturulan tip 2 diyabetes mellitus (T2DM)’de sesamin, saponin, sesamin+saponin, tahin, çöven ektsraktı ve tahin+çöven ektsraktı uygulamalarının aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), kan üre nitrojen (BUN) ve kreatinin düzeylerine etkisini belirlemektir. Araştırmada 48 adet Swiss albino erkek fare kullanıldı. Sekiz adet fare sağlıklı kontrol (SK) grubu için ayrıldıktan sonra diğer hayvanlarda yüksek yağlı besleme ve streptozotosin (STZ) uygulaması yapılarak T2DM oluşturuldu. T2DM oluşturulan 42 adet fare 7 gruba ayrılarak diyabet kontrol (DK), sesamin (D+SS), saponin (D+SP), sesamin+saponin (D+SS+SP), tahin (D+T), çöven ekstraktı (D+Ç) ve tahin+çöven ekstraktı (D+T+Ç) 2 hafta süresince uygulandı. Deney süresi sonunda anestezi altında kanları alınarak serum örnekleri toplanan fareler ötenazi edildi. Serum AST, ALT, BUN ve kreatinin düzeyleri otoanalizörde ölçüldü. AST düzeyi D+SP grubunda yüksek (p<0.05), D+SP, D+T ve DK gruplarında ise birbiri ile benzer bulundu (p>0.05). SK grubu AST düzeyi ise DK, D+SP, D+SS+SP ve D+T gruplarından düşük belirlendi (p<0.05). DK ile D+SP grupları ALT düzeyleri birbiri ile benzer bulunurken (p>0.05), diğer araştırma gruplarından farklı tespit edildi (p<0.05). SK ile DK grupları BUN değerleri birbirleri ile benzer bulunurken (p>0.05), iki grubun BUN değerlerinin diğer gruplardan farklı olduğu belirlendi (p<0.05). SK, DK, D+SP ve D+T gruplarının kreatinin düzeyleri birbirleri ile benzer (p>0.05) olarak tespit edilirken, diğer gruplardan farklı olduğu gözlendi (p<0.05). Sonuç olarak uygulanan maddelerin T2DM’de karaciğer ve böbrek fonksiyonu üzerine olumlu etkileri olabileceği ifade edilebilir.

Keywords

tahin, Çöven, Diyabet

Ethical Statement

Selçuk Üniversitesi Deneysel Tıp Uygulama ve Araştırma Merkezı̇ Hayvan Deneylerı̇ Yerel Etı̇k Kurulu (Tarih: 28/08/2020; Karar No: 2020/36 ve Tarih: 24/09/2021; Karar No: 2020/36(Ek-1))

Supporting Institution

Selçuk Üniversitesi

Project Number

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü, Proje Numarası: 20112005

Effect of Gypsophila Extract, Tahini, Saponin and Sesamin on some Biochemical Parameters in Type 2 Diabetic Mice

Abstract

The study aims to determine the effects of sesamin, saponin, sesamin+saponin, tahini, gypsophila extract and tahini+gypsophila extract on aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine levels in experimentally induced type 2 diabetes (T2DM) in mice. 48 Swiss albino male mice were used. After 8 mice were separated as healthy control (HC) group, T2DM was induced by high-fat feeding and streptozotocin (STZ) application. 42 T2DM mice were divided into 7 groups; diabetes control (DC), sesamin (D+SS), saponin (D+SP), sesamin+saponin (D+SS+SP), tahini (D+T), gypsophila extract (D+C) and tahini+gypsophila extract (D+T+C) were applied for 2 weeks. At the end of the research, the animals were euthanized and serum was obtained. Serum AST, ALT, BUN and creatinine levels were measured on an autoanalyzer. AST level was higher than D+SP group (p<0.05), but like D+SP, D+T and DC groups (p>0.05). The AST level of the HC group was lower than DC, D+SP, D+SS+SP and D+T groups (p<0.05). While the ALT levels of DC and D+SP groups were similar to others (p>0.05), they were different from the other groups (p<0.05). While the BUN values of HC and DC groups were similar (p>0.05), BUN values of the two groups were different from the other groups (p<0.05). While the creatinine levels of HC, DC, D+SP and D+T groups were like each other (p>0.05), they were different from the other groups (p<0.05). As a result, it can be stated that the applied substances may have positive effects on T2DM liver and kidney function.

Keywords

Gypsophila, Diabetes, Tahini

Project Number

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü, Proje Numarası: 20112005

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