Antioxidant and Anti-Radical Potentials of Different Extracts of Koycegiz Propolis: A Comparative Study

Year 2025, Volume: 36 Issue: 2, 146 - 150, 27.07.2025

Hasan Aydın

https://doi.org/10.36483/vanvetj.1703208

Abstract

Propolis is a natural product rich in flavonoids and phenolic compounds produced by the honey bee (Apis mellifera) by combining plant-derived resins and beeswax. Propolis, which has been used in traditional medicine for a long time in the treatment of various diseases, has been scientifically proven to have strong antioxidant properties. In this study, it was aimed to evaluate the antioxidant capacities and free radical scavenging effects of propolis extracts prepared in water, ethanol and glycerol solvents.Ethanol, water and glycerol extracts were obtained from propolis samples collected from Köyceğiz region of Muğla province. Antioxidant capacity in the extracts was determined by CUPRAC and free radical scavenging activity by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. In terms of antioxidant activity, ethanol, water and glycerol extracts of propolis were as follows, respectively; 0.825±0.048, 0.165±0.006, 0.089±0.008 mmol TR/g dry extract was determined. Antiradical activity was found to be the highest in ethanol extract (94.224±0.245%) as in antioxidant capacity. Antiradical activities of water and glycerol extracts were determined as 32.597±1.081% and 23.277±2.458, respectively. In this study, it was observed that ethanol extract of propolis had higher antioxidant capacity and antiradical activity than other water and glycerol extracts.

Keywords

Antioxidant Capacity , Antiradical Activity , CUPRAC , DPPH , Propolis

Köyceğiz Propolisinin Farklı Ekstraktlarının Antioksidan ve Antiradikal Potansiyelleri: Karşılaştırmalı Bir Çalışma

Abstract

Propolis, bal arısı (Apis mellifera) tarafından bitkisel kaynaklı reçineler ile balmumunun bir araya getirilmesiyle üretilen, flavonoid ve fenolik bileşikler bakımından zengin doğal bir üründür. Geleneksel tıpta uzun süredir çeşitli hastalıkların tedavisinde kullanılan propolisin, güçlü antioksidan özellikler taşıdığı bilimsel olarak ortaya konmuştur. Bu çalışma kapsamında, propolisin su, etanol ve gliserol çözücülerinde hazırlanan ekstraktlarının antioksidan kapasiteleri ile antiradikal etkilerinin değerlendirilmesi hedeflenmiştir. Muğla ili Köyceğiz bölgesinden toplanan propolis örneklerinden etanol, su ve gliserol ekstraktları elde edilmiştir. Ekstraktlarda antioksidan kapasite CUPRAC ve serbest radikal süpürücü aktivite 1,1-diphenyl-2-picryl hydrazyl (DPPH) yöntemi ile tespit edilmiştir. Propolis, antioksidan aktivite bakımından etanol, su ve gliserol ekstraktları sırasıyla; 0.825±0.048, 0.165±0.006, 0.089±0.008 mmol TR/g kuru ekstrakt olarak tespit edilmiştir. Antioksidan kapasite de olduğu gibi antiradikal aktivite de etanol ekstraktı (%94.224±0.245) en yüksek olarak tespit edilmiştir. Su ve gliserol ekstraktlarının antiradikal aktiviteleri sırasıyla; %32.597±1.081 ve 23.277±2.458 olarak tespit edilmiştir. Bu araştırmada propolisin etanol ekstraktının diğer su ve gliserol ekstraktlarına göre daha yüksek antioksidan kapasiteye ve antiradikal etkiye sahip olduğu görülmüştür.

Keywords

Antioksidan Kapasite , Antiradikal Aktivite , CUPRAC , DPPH , Propolis.

References

• Abduh MY, Shafitri TR, Elfahmi E (2024). Chemical profiling, bioactive compounds, antioxidant, and anti-inflammatory activities of Indonesian propolis extract produced by Tetragonula laeviceps. Heliyon,10 (19), 1-13.
• Ağrı İli Tutak Kaymakamlığı (2025). İlçe Hakkında Genel Bilgiler.Erişim Tarihi:15 Nisan 2025. Erişim Adresi:http://tutak.gov.tr/ilce-hakkinda-genel-bilgiler.
• Ahn MR, Kumazawa S, Hamasaka T, Bang KS, Nakayama T (2004). Antioxidant activity and constituents of propolis collected in various areas of Korea. J Agric Food Chem, 52 (24), 7286–7292.
• Ahn, MR, Kumazawa S, Usui Y et al. (2007). Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem, 101 (4), 1383–1392.
• Apak R, Güçlü K, Özyürek M, Karademir SE, Erçağ E (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr, 57 (5–6), 292–304.
• Arslan M, Sevgiler Y, Güven C et al. (2021). Chemical and biological characteristics of propolis from Apis mellifera caucasica from the Ardahan and Erzurum provinces of Turkey: A comparative study. Arh Hig Rada Toksikol, 72 (1), 53–69.
• Benedek C, Veresné Bálint M (2022). Characteristics and uses of propolis. Elelmiszervizsgalati, 68 (3), 4028–4035.
• Bozkuş TN (2024). Determination of Phenolic Contents and Antioxidant Activities in Different Solvents of Hatila Valley and Macahel Bee Products in Artvin, Turkey. KSÜ Tarım ve Doğa Derg, 27 (4), 949-957.
• Bozkuş TN¸ Değer O, Yaşar A (2024). Chemical characterization of water and ethanolic extracts of Turkish propolis by HPLC-DAD and GC-MS. Liq Chromatogr Relat Technol, 44 (1-2), 77–86.
• Çakiroğlu TN (2010). Çeşitli Çözücülerde Türk Propolisinin Çözünürlüğünün İncelenmesi. Yüksek Lisans Tezi, Karadeniz Teknik Üniversitesi Sağlık Bilimleri Üniversitesi, Trabzon, Türkiye.
• De Groot AC (2013). Propolis: A review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis, 24 (6), 263–282.
• Demir S, Atayoglu AT, Galeotti F et al. (2021). Antiviral activity of different extracts of standardized propolis preparations against HSV. Antivir Ther, 25 (7), 353–363.
• Donmez M, Karadeniz S, Yoldaş T et al. (2020). Comparison of Chemical Contents of Extracts in Different Solvents of Propolis Samples Produced in Duzce Province. J Tradit Complement Med, (1)3, 137-146.
• Erdogan U (2023). Ultrasonic assisted propolis extraction: characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability. Int J Second Metab, 10 (2), 231–239.
• Gülçin I, Bursal E, Şehitoğlu MH, Bilsel M, Gören AC (2010). Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol, 48 (8–9), 2227–2238.
• Kabiloglu O, Abas I, Kocabaglı N (2025). Effect of propolis extract on performance, health and immune parameters in lambs. Small Rumin Res, 249, 107522.
• Kara Y, Can Z, Kolaylı S (2022). What Should Be the Ideal Solvent Percentage and Solvent‑Propolis ratio in the Preparation of Ethanolic Propolis Extract? Food Anal Methods, 15, 1707–1719.
• Karagecili H, Yılmaz MA, Ertürk A et al. (2023). Comprehensive Metabolite Profiling of Berdav Propolis Using LC-MS/MS: Determination of Antioxidant, Anticholinergic, Antiglaucoma, and Antidiabetic Effects. Molecules, 28 (4), 1739.
• Kehribar L, Coşkun HS, Surucu S, Aydın M, Mahiroğulları M (2021). The Antibacterial Effectiveness of Propolis on Medical Screws. Cureus, 13 (7), e16278.
• Khalaf DA, Thanoon IA-J (2018). Effects of Bee Propolis on Blood PressureRecord and Certain Biochemical Parameter in Healthy Volunteers. Ann Coll Med Mosul, 40 (1), 20-26.
• Kocot J, Kiełczykowska M, Luchowska-Kocot D, Kurzepa J, Musik I (2018). Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application. Oxid Med Cell Longev, 2018, 7074209.
• Kumazawa S, Hamasaka T, Nakayama T (2004). Antioxidant activity of propolis of various geographic origins. Food Chem, 84 (3), 329–339.
• Lagouri V, Prasianaki D, Krysta F (2014). Antioxidant properties and phenolic composition of Greek propolis extracts. Int J Food Prop, 17 (3), 511–522.
• Lim, OZ, Yeoh BS, Omar N et al. (2022). Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy. Braz J Pharm Sci, 58, e19652.
• Miłek M, Bonikowski R, Dżugan M (2024). The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract. Chem Pap, 78, 6709–6720.
• Muğla Orman Genel Müdürlüğü (2025). Erişim Tarihi: 15 Mayıs 2025 Erişim adresi: https://www.ogm.gov.tr/muglaobm/ormanlarimiz/orman-varligi.
• Nichitoi MM, Josceanu AM, Isopescu RD et al. (2021). Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts. Sci Rep, 11, 20113.
• Osés SM, Fernández-Muiño MA, Rodríguez-Fernández A et al. (2024). Phenolic Composition, Antiradical, Antimicrobial, and Anti-Inflammatory Activities of Propolis Extracts from North East Spain. J Med Food, 27 (6), 563–574.
• Özdal HR, Nakilcioğlu E, Ötleş S (2023). Propolisin Biyoaktif Bileşenleri Üzerine Ekstraksiyon Yöntemlerinin ve Ekstraksiyon Değişkenlerinin Etkileri. The Journal of Food, 48 (6), 1123-1131.
• Özenirler Ç, Zare G (2022). Evaluation of the Polyphenol Contents and Antioxidant Activity of Propolis Extracted with Different Techniques. Uludag Bee J, 22 (2), 176-187.
• Paula BV, Estevinho LM, Cardoso SM, Dias LG (2023). Comparative Methods to Evaluate the Antioxidant Capacity of Propolis: An Attempt to Explain the Differences. Molecules, 28, 4847.
• Rahman S, Talukdar NC, Patowary K, Mohanta YK (2024). Propolis from the North-East region of India exhibits potent antioxidant and anticancer activity against breast cancer cells. Biocatal Agric Biotechnol, 59, 103269.
• Saho, R, Swaın CK (2022). Bee Propolis Use in Biotic and Abiotic Compositionsand Its BiologicalOriginforAnticancer Medicines. J Int Res Med Pharm Sci, 17 (3), 22–29.
• Santos LA, Rosalen PL, Dias NA et al. (2021). Brazilian Red Propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis. J Ethnopharmacol, 277, 114181.
• Şenol Yazkan SN, Hendek Ertop M (2024). Use of chestnut propolis extract produced by maseration, reflux and ultrasound-assisted extraction methods in different food models: Effect on physicochemical, sensory and bioactive properties. Food and Health, 10 (1), 22–39.
• Šuran J, Cepanec I, Mašek T et al. (2021). Propolis extract and its bioactive compounds-from traditional to modern extraction technologies. Molecules, 26 (10), 2930.
• Svetikiene D, Zamokas G, Jokubaite M (2024). The Comparative Study of the Antioxidant and Antibacterial Effects of Propolis Extracts in Veterinary Medicine. Vet Sci, 11 (8), 375.
• Touzani S, Al-Waili N, Imtara H et al. (2022). Arbutus Unedo Honey and Propolis Ameliorate Acute Kidney Injury, Acute Liver Injury, and Proteinuria via Hypoglycemic and Antioxidant Activity in Streptozotocin-Treated Rats. Cell Physiol Biochem, 56 (1), 66–81.
• Ufuk S, Sengül M (2023). The effects of different extraction methods and solvents on antioxidant properties of propolis. BSJ Agri, 6 (4), 386-393.
• Vagish Kumar LS (2014). Propolis in dentistry and oral cancer management. N Am J Med Sci, 6 (6), 11–20.
• Wali AF, Mushtaq A, Rehman MU, Akbar S, Masoodi MH (2017). Bee Propolis (Bee’s Glue): A Phytochemistry Review. J Crit Rev, 4 (4), 9-13.
• Walker P, Crane E (1987). Constituents of Propolis. Apidologie, 18 (4), 327–334.
• Woźniak M, Mrówczyńska L, Kwaśniewska-Sip P et al. (2020). Effect of the Solvent on Propolis Phenolic Profile and its Antifungal, Antioxidant, and in Vitro Cytoprotective Activity in Human Erythrocytes under Oxidative Stress. Molecules, 25 (18), 4266.
• Woźniak M, Sip A, Mrówczyńska L et al. (2023). Biological Activity and Chemical Composition of Propolis from Various Regions of Poland. Molecules, 28 (1), 141.