Ratlarda parasetamol ile indüklenen nefrotoksisite modelinde eser elementler ve antioksidan kapasite üzerine borun etkisinin araştırılması

Year 2020, , 25 - 35, 15.01.2020

Ecem Özkan Funda Karabağ

https://doi.org/10.33188/vetheder.557918

Cited By: 2

Abstract

Bu çalışmada öncelikle borun parasetamol indüklü
nefrotoksisite modelinde eser element ve antioksidan kapasite üzerine etkisi
araştırılmıştır. Çalışmanın dayandığı esas hipotez, borun antioksidan etkinliğinin
vücudumuzdaki eser element ve antioksidan kapasite üzerinde etkisinin olup
olmadığını gösterilmesi açısından özgün bir değer taşımaktadır. 56 albino
Wistar ratlar 7 gruba ayrılmıştır: Grup 1 (Kontrol); Grup 2 (parasetamol); Grup
3 (parasetamol + 50 mg/kg bor); Grup 4 (parasetamol + 100 mg/kg bor); Grup 5 (parasetamol
+ 200 mg/kg bor); Grup 6 (parasetamol + 140 mg/kg NAC); Grup 7 (200 mg/kg
bor).Araştırma sonucunda ratlardan alınan doku numuneleri eser element analizi
(Magnezyum,  Mangan, Kobalt,
Bakır, Çinko, Selenyum), Malondialdehit (MDA), total
antioksidan durum (TAS), total oksidan durum (TOS), süperoksit dismutaz
aktivitesi (SOD), katalaz aktivitesi (CAT), glutatyon peroksidaz (GPx)
analizleri için kullanılmıştır. Ayrıca ratların kalplerinden alınan kan
numuneleri de kreatinin ve kandaki üre azotu (BUN) analizleri için incelenmiştir.
Bulgular sonucunda, bu
çalışma borun antioksidan özellik göstererek parasetamol indüklü nefrotoksisite
de eser elementler ve antioksidan kapasite üzerine koruyucu etkisinin olduğunu
destekliyor olabileceğini düşündürtmektedir.

Keywords

bor, parasetamol, nefrotoksisite, sıçan, eser element

Investigation of boron effect on trace elements and antioxidant capacity in paracetamol-induced nephrotoxicity model

Abstract

In this study, the effect of boron on the trace element and antioxidant capacity in paracetamol induced nephrotoxicity model was investigated. The main hypothesis on which the study is based has a unique value in terms of showing whether the antioxidant activity of boron has an effect on the trace elements and antioxidant capacity in our body. In the study, 56 albino Wistar rats were divided into 7 groups: Group 1 (Control); Group 2 (Paracetamol); Group 3 (Paracetamol + 50 mg/kg boron); Group 4 (Paracetamol + 100 mg/kg boron); Group 5 (Paracetamol + 200 mg/kg boron); Group 6 (Paracetamol + 140 mg/kg NAC); Group 7 (200 mg/kg boron). At the end of the research, tissue samples taken from rats were used for trace element analysis (Magnesium (Mg), Manganese (Mn), Cobalt (Co), Copper (Cu), Zinc (Zn), Selenium (Se), Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione peroxidase (GPx) analyses. Likewise, blood samples taken from the hearts of rats were examined for creatinine blood urea nitrogen (BUN) analyses. As a result of the findings, this study suggests that the boron may have antioxidant properties and may have a protective effect on trace elements and antioxidant capacity in paracetamol-induced nephrotoxicity.

Keywords

boron, Paracetamol, nephrotoxicity, trace element, rat

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