Ratlarda parasetamol ile indüklenen nefrotoksisite modelinde eser elementler ve antioksidan kapasite üzerine borun etkisinin araştırılması

Öz

Bu çalışmada öncelikle borun parasetamol indüklü nefrotoksisite modelinde eser element ve antioksidan kapasite üzerine etkisi araştırılmıştır. Çalışmanın dayandığı esas hipotez, borun antioksidan etkinliğinin vücudumuzdaki eser element ve antioksidan kapasite üzerinde etkisinin olup olmadığını gösterilmesi açısından özgün bir değer taşımaktadır. 56 albino Wistar ratlar 7 gruba ayrılmıştır: Grup 1 (Kontrol); Grup 2 (parasetamol); Grup 3 (parasetamol + 50 mg/kg bor); Grup 4 (parasetamol + 100 mg/kg bor); Grup 5 (parasetamol + 200 mg/kg bor); Grup 6 (parasetamol + 140 mg/kg NAC); Grup 7 (200 mg/kg bor).Araştırma sonucunda ratlardan alınan doku numuneleri eser element analizi (Magnezyum,  Mangan, Kobalt, Bakır, Çinko, Selenyum), Malondialdehit (MDA), total antioksidan durum (TAS), total oksidan durum (TOS), süperoksit dismutaz aktivitesi (SOD), katalaz aktivitesi (CAT), glutatyon peroksidaz (GPx) analizleri için kullanılmıştır. Ayrıca ratların kalplerinden alınan kan numuneleri de kreatinin ve kandaki üre azotu (BUN) analizleri için incelenmiştir. Bulgular sonucunda, bu çalışma borun antioksidan özellik göstererek parasetamol indüklü nefrotoksisite de eser elementler ve antioksidan kapasite üzerine koruyucu etkisinin olduğunu destekliyor olabileceğini düşündürtmektedir.

Anahtar Kelimeler

• bor,parasetamol,nefrotoksisite,sıçan,eser element

Investigation of boron effect on trace elements and antioxidant capacity in paracetamol-induced nephrotoxicity model

Abstract

In this study, the effect of boron on the trace element and antioxidant capacity in paracetamol induced nephrotoxicity model was investigated. The main hypothesis on which the study is based has a unique value in terms of showing whether the antioxidant activity of boron has an effect on the trace elements and antioxidant capacity in our body. In the study, 56 albino Wistar rats were divided into 7 groups: Group 1 (Control); Group 2 (Paracetamol); Group 3 (Paracetamol + 50 mg/kg boron); Group 4 (Paracetamol + 100 mg/kg boron); Group 5 (Paracetamol + 200 mg/kg boron); Group 6 (Paracetamol + 140 mg/kg NAC); Group 7 (200 mg/kg boron). At the end of the research, tissue samples taken from rats were used for trace element analysis (Magnesium (Mg), Manganese (Mn), Cobalt (Co), Copper (Cu), Zinc (Zn), Selenium (Se), Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione peroxidase (GPx) analyses. Likewise, blood samples taken from the hearts of rats were examined for creatinine blood urea nitrogen (BUN) analyses. As a result of the findings, this study suggests that the boron may have antioxidant properties and may have a protective effect on trace elements and antioxidant capacity in paracetamol-induced nephrotoxicity.

Keywords

• boron,Paracetamol,nephrotoxicity,trace element,rat

Kaynakça

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