Comparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality

Year 2021, , 42 - 48, 15.01.2021

Ali Taşkın , Ahmet Kocabay , Şeref Gül , Kübra Çağlar Erkal

https://doi.org/10.33188/vetheder.789268

Abstract

The aim of our research is to investigate the effects of parthenogenetic activation on in vitro embryo development rates in different mouse strains. B6CBAF1, C57BL/6j, and B6D2F1 mouse strains were used in this study. Superovulated mice were sacrificed and oocytes were obtained 14 hours after the human chorionic gonadotrophin (hCG) injection and the parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated for 6 hours in 10 mM SrCl2 + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing Ca2+ free Chatot Ziomek Brinster (CZB) activation medium. After this, further incubation was performed for two hours in an incubator at 37 °C and 5% CO2 in embryo culturing medium + TSA. Finally, embryos were cultured for 120 hours. Parthenogenetic activation success of the B6D2F1 mouse strain was found to be higher than C57BL/6j and B6CBAF1 strains.

Keywords

Fare, Oosit, Parthenogenetic, Activation, Embryo Culture

Supporting Institution

TUBITAK - The Scientific and Technological Research Council of Turkey

Project Number

Grant Number: TOVAG 114O638

Thanks

The authors gratefully acknowledge the use of the services and facilities of the Koç University Research Center for Translational Medicine (KUTTAM), funded by the Republic of Turkey Ministry of Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Ministry of Development. This research was supported by a grant from TUBITAK - The Scientific and Technological Research Council of Turkey (Grant Number: TOVAG 114O638).

Farklı fare ırklarında parthenogenotik oosit aktivasyonun in vitro gelişim oran ve kalitesinin karşılaştırılması

Abstract

Çalışmamızın amacı, partenogenetik aktivasyonda farklı fare ırklarında in vitro embriyo gelişimi ve kalitesi üzerindeki etkilerinin araştırılmasıdır. Bu çalışmada, B6CBAF1, C57BL/6j, and B6D2F1 farelerin superovulasyon ile elde edilen oositleri kullanılmıştır. Superovule edilen fareler, insan koryonik gonadotropin (hCG) uygulamasından 14 saat sonra oositler elde edildi ve 18 saat sonra partenogenetik aktivasyona başlandı. Oositler, 10 mM SrCl2 + 5 μg/mL-1 sitokalazin B (CB) + 5 nM trikostatin A (TSA) Ca 2+ içermeyen Chatot Ziomek Brinster (CZB) medyumu içerisinde 6 saat bekletildi. Aktivasyon sonrası, embriyo kültür medyumu + TSA’da inkübatörde 37°C ve %5 CO2 ortamında 2 saat bekletildi. Son olarak, tüm embriyolar 120 saat süre ile kültüre edildi. Bu çalışmadan elde edilen sonuçlar göre, B6D2F1 ırkının partenogenetik aktivasyon başarısı, C57BL/6j ve B6CBAF1 ırklarına göre daha yüksek bulundu.

Keywords

Fare, Oosit, Parhenogenetik, Aktivasyon, Embriyo kültür

Project Number

Grant Number: TOVAG 114O638

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