Farklı fare ırklarında parthenogenotik oosit aktivasyonun in vitro gelişim oran ve kalitesinin karşılaştırılması

Öz

Çalışmamızın amacı, partenogenetik aktivasyonda farklı fare ırklarında in vitro embriyo gelişimi ve kalitesi üzerindeki etkilerinin araştırılmasıdır. Bu çalışmada, B6CBAF1, C57BL/6j, and B6D2F1 farelerin superovulasyon ile elde edilen oositleri kullanılmıştır. Superovule edilen fareler, insan koryonik gonadotropin (hCG) uygulamasından 14 saat sonra oositler elde edildi ve 18 saat sonra partenogenetik aktivasyona başlandı. Oositler, 10 mM SrCl2 + 5 μg/mL-1 sitokalazin B (CB) + 5 nM trikostatin A (TSA) Ca 2+ içermeyen Chatot Ziomek Brinster (CZB) medyumu içerisinde 6 saat bekletildi. Aktivasyon sonrası, embriyo kültür medyumu + TSA’da inkübatörde 37°C ve %5 CO2 ortamında 2 saat bekletildi. Son olarak, tüm embriyolar 120 saat süre ile kültüre edildi. Bu çalışmadan elde edilen sonuçlar göre, B6D2F1 ırkının partenogenetik aktivasyon başarısı, C57BL/6j ve B6CBAF1 ırklarına göre daha yüksek bulundu.

Anahtar Kelimeler

• Fare
• Oosit
• Parhenogenetik
• Aktivasyon
• Embriyo kültür

Comparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality

Abstract

The aim of our research is to investigate the effects of parthenogenetic activation on in vitro embryo development rates in different mouse strains. B6CBAF1, C57BL/6j, and B6D2F1 mouse strains were used in this study. Superovulated mice were sacrificed and oocytes were obtained 14 hours after the human chorionic gonadotrophin (hCG) injection and the parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated for 6 hours in 10 mM SrCl2 + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing Ca2+ free Chatot Ziomek Brinster (CZB) activation medium. After this, further incubation was performed for two hours in an incubator at 37 °C and 5% CO2 in embryo culturing medium + TSA. Finally, embryos were cultured for 120 hours. Parthenogenetic activation success of the B6D2F1 mouse strain was found to be higher than C57BL/6j and B6CBAF1 strains.

Keywords

• Fare
• Oosit
• Parthenogenetic
• Activation
• Embryo Culture

Kaynakça

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