Validity Control of Markers Used in Molecular Marker Assisted Selection in Tomato

Year 2022, , 300 - 309, 30.06.2022

Ceylan Uçar , Suat Şensoy

https://doi.org/10.29133/yyutbd.1039398

Cited By: 2

Abstract

This study aimed to check the validity of the SCAR and CAPS markers developed for certain diseases and pests on some tomato cultivars and genotypes in molecular marker-assisted selection. For this purpose, developed molecular markers for resistance were tested for tomato wilt virus (TSWV), Fusarium wilt (FOL), Tomato leaf curl virus (TYLCV), and root-knot nematode (RKN). SCAR Scr-001 markers for TSWV, TAO1 CAPS marker, and P743DF1-P743DR1, P743DF3- SCAR P6-25 markers for FOL; Scar P6-25 marker which TYLCV; SCAR Mi-23 and PMI, of RKN, CAPS APS and C8B markers for P743DR3, P743DF1-P743DR1, At2F- ToMV were selected. These selected markers were screened in 24 tomato genotypes, 9 of which were commercial and 12 local genotypes as well as the control group, Mountain Merit, NCICELBR, and NCI123S. SCAR Scr-001 marker for TSWV; TAO1 CAPS marker and P743DF3-P743DR3, P743DF1-P743DR1, At2-F-At2-R SCAR markers for FOL; P6-25 SCAR marker for TYLCV; and SCAR Mi-23 and PMI markers for RKN gave results. In this context, it was concluded that the mentioned markers could be efficiently used in marker-assisted selection studies in tomatoes.

Keywords

CAPS, SCAR, Solanum lycopersicum L, Molecular marker assisted selection

Project Number

2015-FBE-YL277

References

• Agrios, G. N. (1997). Genetics of plant disease. Plant Pathology, 115-142.
• Aydınlı, G. (2015). Domateste kök-ur nematodları (Meloidogyne spp.)’na dayanıklılık: Mi geni ve virülent popülasyon oluşumu. Derim, 32(2), 211-224.
• Anfoka, G. H., Abhary, M., & Stevens, M. R. (2006). Occurrence of Tomato spotted wilt virus (TSWV) in Jordan. EPPO Bulletin, 36(3), 517-522.
• Arens, P., Mansilla, C., Deinum, D., Cavellini, L., Moretti, A., Rolland, S., & Mathis, R. (2010). Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing. Theoretical and Applied Genetics, 120(3), 655-664.
• Azeri, T. (1981). Preliminary report of tomato spotted wilt virus (TSWV) and its epidemy on tobacco in the Canakkale region of Turkey. Journal of Turkish phytopathology. 10(2/3), 79-87.
• Barillas, A. C., Mejia, L., Sanchez-Perez, A., & Maxwell, D. P. (2008). CAPS and SCAR markers for detection of I-3 gene introgression for resistance to Fusarium oxysporium f. sp. lycopersici race 3. Rep Tomato Genetic. Coop, 58, 11-17.
• Barone, A., & Frusciante, L. (2007). Molecular marker-assisted selection for resistance to pathogens in tomato. Marker-Assisted Selection, Current Status and Future Perspectives in Crops, Livestock, Forestry and Fish, 153-164.
• Bayram, M., Sensoy, S., & Dasgan, H. Y. (2010). August. Iron (Fe) deficiency tolerance in tomato recombinant inbred lines obtained from tolerant and sensitive genotypes. In XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium 918, 575-582.
• Chen, H. M., Lin, C. Y., Yoshida, M., Hanson, P., & Schafleitner, R. (2015). Multiplex PCR for detection of tomato yellow leaf curl disease and root-knot nematode resistance genes in tomato (Solanum lycopersicum L.). International Journal of Plant Breeding and Genetics, 9(2), 44-56.
• Cho, JJ., Mau, RFL., German, TL., Hartmann, RW., Yudin, LS., Gonsalves, D., & Provvidenti, R. (1989). A multidisciplinary approach to management of Tomato spotted wilt virus in Hawaii. Plant Disease, 73, 375-383.
• Collard, B. C., & Mackill, D. J. (2008). Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 363(1491), 557-572.
• Danso, Y., Akromah, R., & Osei, K. (2011). Molecular marker screening of tomato, (Solanum lycopersicum L.) Germplasm for root-knot nematodes (Meloidogyne species) resistance. African Journal of Biotechnology, 10(9), 1511-1515.
• Dax, E., Livneh, O., Aliskevicius, E., Edelbaum, O., Kedar, N., Gavish, N., & Sela, I. (1998). A SCAR marker linked to the ToMV resistance gene, Tm2 2, in tomato. Euphytica, 101(1), 73-77.
• Devran, Z., & Elekçioğlu, İ. H. (2004). The Screening of F2 Plants for the root-knot nematode resistance gene, Mi by PCR in tomato. Turkish Journal of Agriculture and Forestry, 28(4), 253-257.
• De Castro, A. P., Blanca, J. M., Díez, M. J., & Vinals, F. N. (2007). Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene Ty-1 in tomato. European Journal of Plant Pathology, 117(4), 347-356.
• Doyle, J. J. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
• El Mehrach, K., Hatimi, A., Gharsallah Chouchane, S., Salus, M. S., Martin, C. T., Maxwell, D. P., & Vidavski, F. (2005). PCR-based methods for tagging the Mi-1 locus for resistance to root-knot nematode in begomovirus-resistant tomato germplasm. Acta Horticulture. 695, 263-270.
• FAO. (2016). Statistic database. http://faostat.fao.org/. Erişim tarihi: 10.07.2017.
• Foolad, M., Zhang, L., Khan, A. A., Nino-Liu, D., & Lin, G. (2002). Identification of QTLs for early blight (Alternaria solani) resistance in tomato using backcross populations of a Lycopersicon esculentum× L. hirsutum cross. Theoretical and Applied Genetics, 104(6-7), 945-958.
• Garcia, B. E., Mejia, L., Salus, M. S., Martin, C. T., Seah, S., Williamson, V. M., & Maxwell, D. P. (2007). A co-dominant SCAR marker, Mi23, for detection of the Mi-1.2 gene for resistance to root-knot nematode in tomato germplasm. PCR-Based Markers Protocols. Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706 pp:1-13.
• Garland, S., Sharman, M., Persley, D., & McGrath, D. (2005). The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Australian Journal of Agricultural Research, 56(3), 285-289.
• Gözel, U., Çiğdem, Y. U. R. T., & Özel Ç. (2016). Molecular markers used in nematode taxonomy. Turkish Bulletin of Entomology, 6(2), 179-189.
• Grube, R. C., Radwanski, E. R., & Jahn, M. (2000). Comparative genetics of disease resistance within the Solanaceae. Genetics, 155(2), 873-887.
• Güldür, M. E. (1997). Şanlıurfa ili için yeni bir virüs: Domates lekeli solgunluk virüsü (TSWV). Harran Üniv. Ziraat Fak. Dergisi, 1(3), 71-76.
• Güllü, M., & Çalı, S. (1994). Doğu Akdeniz Bölgesi örtüaltı sebze alanlarında görülen virüs hastalıklarının belirlenmesi üzerinde araştırmalar. Bitki Koruma Bülteni. 34 (3-4), 79-93.
• Iberkleid, I., Ozalvo, R., Feldman, L., Elbaz, M., Patricia, B., & Horowitz, S. B. (20149. Responses of tomato genotypes to avirulent and Mi-virulent Meloidogyne javanica isolates occurring in Israel. Phytopathology, 104(5), 484-496.
• Jiang, G., Qu, W., Cruz, Y., Chang, C. J., Ho, G. Y., Klein, R. S., & Burk, R. D. (1997). PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems. Journal of clinical microbiology, 35(6), 1304-1310.
• Kaur, S., Dhaliwal, M. S., Cheema, D. S., Jindal, S. K., & Gaikwad, A. K. (2014). Screening of Tomato (Solanum lycopersicum L.) Germplasm for Root-Knot Nematode Resistance using Conventional and Molecular Marker Technicus. Indian Journal of Nematology, 44(1), 56-61.
• Kuklev, M. Y., Fesenko, I. A., & Karlov, G. I. (2009). Development of a CAPS marker for the Verticillium wilt resistance in tomatoes. Russian Journal of Genetics, 45(5), 575-579.
• Kumar, P., Gupta, V. K., Misra, A. K., Modi, D. R., & Pandey, B. K. (2009). Potential of molecular markers in plant biotechnology. Plant Omics, 2(4), 141.
• Luna, A. P., Morilla, G., Voinnet, O., & Bejarano, E. R. (2012). Functional analysis of gene-silencing suppressors from tomato yellow leaf curl disease viruses. Molecular Plant-Microbe İnteractions, 25(10), 1294-1306.
• Özbek, A. G. (2016). Development of High-yielding and Disease Resistant Processing Tomato Lines Using Molecular Marker Technology Master's thesis, İzmir Institute of Technology
• Özarslandan, A., Ekbiç, E., & Elekçioğlu, İ.H. (2010). Domateste kök-ur nematodu ( Meloidogyne javanic a (Treub, 1885) Chitwood)’na dayanıklılık sağlayan Mi 1.2 geninin Mi23 SCAR markırı ile belirlenmesi. Türkiye Entomoloji Dergisi, 35(4), 677-686.
• Park, Y.H., Lee, Y.J., Kang, J.,Choi, Y.W., & Son, B. (2008). Development of gene-based DNA marker for verticillium wilt resistance in tomato. Korean Journal of Horticultural Science & Technology 26(3), 313-319.
• Pandey, K. K., Pandey, P. K., Kalloo, G., & Banerjee, M. K. (2003). Resistance to early blight of tomato with respect to various parameters of disease epidemics. Journal of General Plant Pathology, 69(6), 364-371.
• Pegg, G. F., & Brady, B. L. (2002). Verticillium wilts. CABI Publishing, New York, ISBN 085199 529 2, USA. 541.
• Pınar, H., Atilla, A., Keleş, D., Mutlu, N., Denli, N., & Mustafa, Ü. (2013a). Domates hatlarında Fusarium oxysporum f. sp. lycopersici’ye dayanıklılığın moleküler markörler yardımıyla belirlenmesi. Derim, 30(1), 15-23.
• Pınar, H., Atilla, A., Keleş, D., Mutlu, N., Denli, N., & Mustafa, Ü. (2013b). Domateste bazı hastalık ve zararlılara dayanıklı hat ve çeşit geliştirmede moleküler markörlerin kullanımı. Alatarım, 12(1), 10-18.
• Prasanna, H. C., D. P., Rai, G.K, Krishna, R., Kashyap, S.P., Singh, N. K., & Malathi, V.G. (2015). Pyramiding Ty-2 ve Ty-3 genes for resistance to monopartite and bipartite tomato leaf curl viruses of India. Plant Pathology, 64(2), 256-264.
• Roselló, S., Ricarte, B., José Díez, M., & Nuez, F. (2001). Resistance to tomato spotted wilt virus introgressed from Lycopersicon peruvianum in line UPV 1 may be allelic to Sw-5 and can be used to enhance the resistance of hybrids cultivars. Euphytica, 119(3), 357-367.
• Staniaszek, M., Kozik, E. U., & Marczewski, W. (2007). A. CAPS marker TAO1902 diagnostic for the I-2 gene conferring resistance to Fusarium oxyporum f.sp. lycopersici race 2 in tomato. Plant Breeding, 126(3), 331-333.
• Scott, J. W., Agrama, H. A., & Jones, J. P. (2004). RFLP-based analysis of recombination among resistance genes to Fusarium wilt races 1, 2, and 3 in tomato. Journal of the American Society for Horticultural Science, 129(3), 394-400.
• Sönmezoğlu, Ö. A., Yıldırım, A., Güleç, T. E., & Kandemir, N. (2010). Markör Destekli Seleksiyonun Bugday Islahında Kullanımı. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, 27(1), 105-112.
• Şevik, M. A. (2015). Sebze Üretimini Tehdit Eden Viral Hastalık Etmeni: Domates lekeli solgunluk virüsü (Tomato spotted wilt virus–TSWV). Iğdır Üniversitesi Fen Bilimleri Enst. Dergisi 5(2), 17-23.
• Vardar-Kanlıtepe, Ç., Aras, S., & Cansaran-Duman, D. (2010). Bitki ıslahında moleküler belirteçlerin kullanımı ve gen aktarimi. Türk Hijyen ve Deneysel Biyoloji Dergisi, 67(1), 33-34.
• Şalk, A., Arın, A., Deveci, M., & Polat, S. (2008). Özel Sebzecilik. NKÜ, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Tekirdağ, 488.
• Tanksley, S. D., Young, N. D., Paterson, A. H., & Bonierbale, M. W. (1989). RFLP mapping in plant breeding: new tools for an old science. Nature Biotechnology, 7(3), 257-264.
• Tekinel, N., Dolar, MS., Sağsöz, S., & Salcan, Y. (1969). Mersin Bölgesinde ekonomik bakımdan önemli bazı sebzelerin virüsleri üzerinde araştırmalar. Bitki Koruma Bülteni, 9, 37-49.
• TÜİK. (2016). Türkiye istatistik kurumu. Bitkisel üretim istatistikleri. http://www.tuik.gov.tr/. Erişim tarihi: 10.07.2017.
• Uzun, A. (2010). Gen teknolojisi ve Biyogüvenlik Türk Bilimsel Derlemeler Dergisi 3(2), 67-79.
• Yıldırım, A., & Kandemir, N. (2001). Genetik markörler ve analiz metodları. Özcan, S. Gürel, E., Babaoğlu, M. Bitki Biyoteknolojisi II. Genetik Mühendisliği ve Uygulamaları. Selçuk Üniv. Vakıf Yayınları, 112-159.
• Yudin, L. S., Cho, J. J., & Mitchell, W. C. (1986). Host range of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), with special reference to Leucaena glauca Environmental Entomology,15(6), 1292-1.