An Alternative Live Food for Fish Larvae; Vinegar Eels (Turbatrix aceti) Enriched with Bacterial Protein

Abstract

Especially at commercial rearing of marine and ornamental fish species, the achievement of early stage feeding operations is very significant for reducing larval loses. For this reason, it is necessary to produce and use of live food organisms that appropriate for first mouth gap sizes of fish larvae. Widely used in this regard rotifer (Brachionus plicatilis) has got uneasy culture procedures, so evaluating alternative live food organisms is very important. In this study, we have assessed the feasibility of vinegar eels in early stage feeding of altricial fish larvae. Zebra fish (Danio rerio) have got smaller mouth gap size than other fish species have been used in feeding trials and acceptabilities of vinegar eels by larvae was evaluated. And also, an increase in protein contents of vinegar eel evaluated using bacterial proteins (Lactococcus garvieae).

Keywords

• Bacterial protein,Fish larvae,L. garvieae,Real-Time PCR,Vinegar eels,Zebra fish

Balık Larvaları için Alternatif bir Canlı Yem; Bakteriyel Protein ile Zenginleştirilmiş Sirke Kurtları (Turbatrix aceti)

Abstract

Özellikle deniz ve süs balıkları türlerinin ticari olarak yetiştirilmesinde erken evre besleme işlemlerinin başarılması larva kayıplarını azaltmak için çok önemlidir. Bu nedenle, ilk ağız boşluğu büyüklüğünde balık larvaları için uygun canlı gıda organizmalarının üretilmesi ve kullanılması gerekmektedir. Bu konuda yaygın olarak kullanılan rotifer (Brachionus plicatilis), kolay olmayan kültür prosedürlerine sahiptir, bu nedenle alternatif canlı gıda organizmalarının değerlendirilmesi çok önemlidir. Bu çalışmada altrisayal balık larvalarının erken beslenme evresinde sirke kurdunun canlı yem olarak uygulanabilirliği denenmiştir. Diğer balık türlerinin beslenme denemelerinde kullanılandan daha küçük ağız boşluğu büyüklüğüne sahip olan Zebra balığı (Danio rerio)’nın sirke kurtlarını canlı yem olarak kabul edilebilirlikleri değerlendirilmiştir. Sirke kurtlarının beslenmesinde ise bakteriyel (Lactococcus garvieae) proteinlerin kullanımı değerlendirilmiştir.

Keywords

• Sirke kurdu,Bakteriyel protein,L. garvieae,Balık larvası,Real-Time PCR,Zebra balığı

References

1. Aas, T. S., Hatlen, B., Grisdale-Helland, B., Terjesen, B. F., Bakke-McKellep, A. M., & Helland, S. J. (2006). Effects of diets containing a bacterial protein meal on growth and feed utilisation in rainbow trout (Oncorhynchus mykiss). Aquaculture, 261(1), 357-368.
2. Altinok, I., Balta, F., Capkin, E., & Kayis, S. (2007). Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture, 273(4), 393-397.
3. Austin, B., & Austin, D. A. (1999). Bacterial Fish Pathogens: Diseases of Farmed and WildFish, 3rd ed. Springer-Praxis, London. 457 pp.
4. Bark, S., & McGregor, D. (2001). The first occurrence of lactococcosis in farmed trout in England. Trout News, 31, 9-11.
5. Carson, J., Gudkovs, N., & Austin, B. (1993). Characteristics of an Enterococcus-like bacterium from Australia and South Africa, pathogenic for rainbow trout (Oncorhynchus mykiss, Walbaum). J. Fish Dis., 16, 381-388.
6. Çağırgan H., (2007). Rainbow trout diseases. Eastern Anatolia Development Program, Agriculture and Rural Development Component, Van, 8-9pp.
7. Çağırgan, H. (2004). Biotyping of Lactococcus garvieae Isolated from Turkey. E.U. Journal of Fisheries & Aquatic Sciences, 21, 267-269.
8. Çevik, M., & Önalan, Ş., (2019). Bazı fitokimyasalların Yersinia ruckeri üzerine etkileri ve antimikrobiyal direnç durumlarının araştırılması. (Yüksek Lisans Tezi). Van Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Van.

9. Diler, Ö., Altun, S., Adiloglu, Kubilay, A. K., & A., Isıklı, B. (2002). First occurrence of Streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull. Eur. Ass. Fish Pathol., 22, 21-26.
10. Duman, M., Saticioglu, I. B., Buyukekiz, A. G., Balta, F., & Altun, S. (2017). Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss). Journal of global antimicrobial resistance, 10, 136-142.
11. Evans, J. J., Klesius, P. H., & Shoemaker, C. A. (2009). First isolation and characterization of Lactococcus garvieae from Brazilian Nile tilapia, Oreochromis niloticus (L.), and pintado, Pseudoplathystoma corruscans (Spix & Agassiz). Journal of fish diseases, 32(11), 943-951. doi: 10.1111/j.1365-2761.2009.01075.
12. Eyngor, M., Zlotkin, A., Ghittino, C., Prearo, M., Douet, D. G., Chilmonczyk, S., & Eldar A. (2004). Clonality and Diversity of the Fish Pathogen Lactococcus garvieae in Mediterranean Countries. Applied and Environmental Microbiology, 70 (9), 5132–5137. doi: 10.1128/AEM.70.9.5132-5137.
13. Francis-West, P. H., Robertson, K. E., Ede, D. A., Rodriguez, C., Izpisua-Belmonte, J. C., Houston, B., Burt, D. W., Gribbin, C., Brickell, P. M., & Tickle, C. (1995). Expression ofgenes encoding bone morphogenetic proteins and sonic hedgehog in talpid(ta3) limb buds: their relationships in the signalling cascade involved in limbpatterning. Dev. Dyn., 203(2), 187–197.
14. Gehenio, P. M., & Luyet, B. J. (1951). Effect of a preliminary slight dehydration on the survival of 'vinegar eels' frozen at -77° C. Biodynamica, 7(130), 41-52.
15. Ghittino, C., & Prearo, M. (1992). Report of Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Italy: preliminary note. Boll. Soc. Patol. Ittica., 8, 4-9.
16. Gorgisen, G., Ozes, D., Pehlivanoglu, S., Erdogan, A., Dertsiz, L., Ozbilim, G., Ozbudak, I. H., Savas, B., & Ozes, O. N. (2013). Differential expression and activation of Epidermal Growth Factor Receptor 1 (EGFR1), ERK, AKT, STAT3, and TWIST1 in nonsmall cell lung cancer (NSCLC). Experimental Lung Research, 39(9), 387-398,doi: 10.3109/01902148.2013.831960.
17. Høiby, N., Bjarnsholt, T., Givskov, M., Molin, S., & Ciofu, O. (2010). Antibiotic resistance of bacterial biofilms. International journal of antimicrobial agents, 35(4), 322-332.
18. Lamason, R. L., Mohideen, M. A., Mest, J. R., Wong, A. C., Norton, H. L., & Aros, M. C. (2005). SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans. Science, 310, 1782–1786.
19. Lee, S. Y., Vedamuthu, E. R., Washam, C. J., & Reinbold, G. W. (1974). An agar medium for the differential enumeration of yogurt starter bacteria. Journal of Milk and Food Technology, 37(5), 272-276.
20. Olguín, E. J. (2012). Dual purpose microalgae–bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a Biorefinery. Biotechnology advances, 30(5), 1031-1046.
21. Øverland, M., Skrede, A., & Matre, T. (2001). Bacterial protein grown on natural gas as feed for pigs. Acta Agriculturae Scandinavica, Section A-Animal Science, 51(2), 97-106.
22. Önal, U., Topaloğlu, G., & Sepil, A. (2015). The Performance of Continuous Rotifer (Brachionus plicatilis) Culture System for Ornamental Fish Production. Journal of Life Sciences, 9, 207-213. doi: 10.17265/1934-7391/2015.05.003.
23. Önalan, Ş., & Yavuz, H. İ. (2019). Nanopartikül içerikli sıvı besiyerlerinde Lactococcus garvieae izolatlarının gelişim farklılıklarının spektrofotometrik olarak belirlenmesi. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 5(1), 6-15.
24. Robin, J. H., & Vincent, B. (2003). Microparticulate diets as first food for gilthead sea bream larva (Sparus aurata): study of fatty acid incorporation. Aquaculture, 225(1-4), 463-474.
25. Savvidis, G. K., Anatoliotis, C., Kanaki, Z., & Vafeas, G. (2007). Epizootic outbreaks of Lactococcosis disease in rainbow trout, Oncorhynchus mykiss (Walbaum), culture in Greece. Bull. Eur. Ass. Fish Pathol., 27(6), 223-228.
26. Vendrell, D., Balca ́zar, J. L., Ruiz-Zarzuela, I., Blas, D., Girones, O., & Muzquiz, J. L. (2006). Lactococcus garvieaein fish: A review. Comparative Immunology, Microbiology& Infectious Diseases, 29, 177-198. doi:10.1016/j.cimid.2006.06.003.
27. Williams, C. M., Lee, C. G., Garlich, J. D., & Shih, J. C. (1991). Evaluation of a bacterial feather fermentation product, feather-lysate, as a feed protein. Poultry Science, 70(1), 85-94.