SP-2 (Prunus spinosa) Klonal Anaç Adayının in vitro Rejenerasyonu Üzerine Farklı Bitki Büyüme Düzenleyici Konsantrasyonlarının Etkileri

Year 2018, Volume: 28 Issue: 1, 36 - 41, 30.03.2018

Esra Bulunuz Palaz Remzi Uğur

https://doi.org/10.29133/yyutbd.334593

Abstract

Bu çalışma, Kahramanmaraş doğal ortamından seleksiyon ıslahıyla elde
edilen klonal anaç adayı SP-2 (Prunus
spinosa) (Uğur ve Paydaş Kargı 2017)’nın doku kültürüyle üretilebilme
olanaklarının araştırılması amacıyla 2017 yılında yürütülmüştür. Çalışmada, MS (Murashige
and Skoog) besi ortamı ve bu ortama ilave edilen iki farklı hormon ve bitki
büyüme düzenleyici kombinasyonları MS-1 (TDZ 0.75 mg/l, NAA 0.5 mg/l, GA₃ 0.5
mg/l, 0.5 mg/l) MS-2 (TDZ 1.5 mg/l, NAA 1.5 mg/l, GA₃ 0.5 mg/l, 0.5
mg/l ),  kontrol olarak ise MS-0
(Hormonsuz normal MS) kullanılmıştır. Çalışma sonunda explant başına en yüksek
kardeş bitkicik sayısının MS-1 ortamından (12.60 adet/explant) ve en uzun
sürgün uzunluğunun 14.19 mm değeriyle MS-0 ortamından elde edildiği
saptanmıştır. Çalışma sonunda en yüksek kardeşlenme oranının elde edildiği ve
daha az miktarda hormonun kullanıldığı MS1 ortamının, MS2 ortamına göre daha
başarılı ve ekonomik olduğuna karar verilmiştir.

Keywords

Anaç, Erik, in vitro, Prunus spinosa

Effects of Different Concentrations of Plant Growth Rugulators on in vitro Regeneration of SP-2 (Prunus spinosa) Clonal Candidate Rootstock

Abstract

Progagation possibilities in vitro
conditions with applied  plant growth
regulators concentration  of SP-2 (Prunus spinosa) (Ugur and Paydas Kargi 2017)
candidate rootstock which obtained by selection breeding programm in
Kahramanmaras natural environment  was
determined in this study at 2017 year. MS (Murashige and Skoog) medium, and this
medium added two different plant growth regulator combinations MS-1 (TDZ 0.75 mg L^-1, NAA 0.5 mg L^-1, Ga3 0.5 mg
L^-1 0.5 mg L^-1) MS-2 (TDZ 1.5 mg L^-1, NAA 1.5 mg
L^-1, Ga3 0.5 mg L^-1, 0.5 mg L^-1) and MS-0
(normal MS without any hormone) as control was used material. Maximum cumulated
shoots per explant at the MS-1 medium (12.60 pieces explant^-1) and
longest shoot lenght at the MS-0 medium (14.19 mm) was found.It was decided
that would be MS-1 medium more economic than MS-2 medium because less  amount of hormones used.

Keywords

Rootstock, Plum, in vitro, Prunus spinosa

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