The Use of DNA Barcoding in Entomology

Year 2018, Volume: 28 Issue: 1, 126 - 134, 30.03.2018

Gökhan Yatkın Nurper Güz

https://doi.org/10.29133/yyutbd.350146

Cited By: 1

Abstract

DNA barcoding is a
taxonomic method that uses a short and standard genomic DNA fragment to
discriminate species. An approximately 600-700 base pair (bp) region of the
mitochondrial cytochrome c oxidase
subunit I (COI) gene referred as
‘DNA barcode’ is used in insect barcoding studies. This short standart DNA
sequence is not only a tool for simplifying species identification but also is
used for discovery of new species. Furthermore DNA barcoding enables the
detection of cryptic species via delimiting species boundaries. Here we
reviewed the historical and conceptual perspective of DNA barcoding term, the
gene regions used as DNA barcodes and COI
gene which is ideal species-identification
marker in insects. Furthermore we
discussed the advantages and limitations of DNA barcoding and presented DNA
barcoding studies in entomology.

Keywords

COI, DNA barcode, Entomology, Taxonomy, Species identification

Entomolojide DNA Barkodlama Tekniğinin Kullanımı

Abstract

DNA barkodlama, herhangi
bir organizmaya ait DNA’nın PCR teknikleri ile çoğaltılacak büyüklükteki kısa
ve standart bir fragmanının dizilenmesi ile tanılanmasını sağlayan taksonomik
bir yöntem olarak ifade edilebilir. Bu amaçla böceklerle yapılan çalışmalarda
‘DNA barkodu’ olarak bilinen mitokondriyal sitokrom
c oksidaz alt ünite I (COI)
geninin yaklaşık 600-700 baz çiftine (bç) ait kısmı kullanılmaktadır. Bu kısa
standart DNA sekansı türlerin tanılanmasını kolaylaştırmasının yanı sıra yeni
türlerin keşfi için de oldukça iyi bir araçtır. Ayrıca DNA barkodlama türlerin
sınırlarını belirleyerek kriptik türlerin ayrımına katkı sağlar. Bu derlemede
DNA barkodlama teriminin tarihsel ve kavramsal olarak ortaya çıkışı, DNA barkodu
olarak kullanılan gen bölgeleri ve böcekler için ideal tür tanımlama markörü
olan COI gen bölgesi hakkında bilgi
verilmiştir. Son olarak DNA barkodlamanın avantajları ve kısıtlamaları
irdelenerek entomolojide DNA barkodlama çalışmalarından örnekler sunulmuştur. 

Keywords

Entomoloji, DNA barkodlama, COI, Taksonomi, Tür tanımlama

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