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Analysis and Diagnosis of Mycovirus dsRNA in Verticillium dahliae, The Causal Agent of Verticillium Wilt Disease of Cotton

Year 2019, Volume: 29 Issue: 3, 516 - 525, 30.09.2019
https://doi.org/10.29133/yyutbd.496476

Abstract

Verticillium
dahliae

(Kleb), one of the important diseases that causes significant yield losses wherever
the cotton and olive were produced. Because the pathogen is soil-borne which
makes the management of the disease difficult. Therefore, biological control in
fungal diseases is becoming more essential, one of them is use the mycovirus
dsRNAs. Three studies on Verticillium
dahliae
mycoviral dsRNAs have been carried out so far, two of them in cotton
and one in olives. In Turkey, no studies were found on the presence of
mycoviral dsRNA in V. dahliae, which
causes cotton wilting. In this study, the presence of mycovirus dsRNA in 72 V. dahliae isolates obtained from the
cotton fields of Aydn/Söke and Koçarlı districts was investigated method. In 12
V. dahliae isolates dsRNA was
determined. The specific primers and random hexamer primer designed for the
mycovirus of V. dahliae (VdPV1-ol) in
olive were used in RT-PCR identification of this determined dsRNA. These primers
have been reported to have identical genome sequences with V. dahliae micovirus (VdPV1) in cotton. Furthermore, V. dahliae isolates containing dsRNA did
not respond to the apple pathogenicity test.

References

  • Adams GC, Hammer SA (1989) Optimum sample size for detecting virulence in Leucostoma isolates from peach. Plant Disase 73: 754-759.Agrıos GN (1997) Plant Pathology. Academik Press. 635 p. Florida.Al-Rawahı AK, Hancock JG (1998( Parasitism and biological control of Verticillium dahliae by Pythium oligandrum. Plant Disease 82: 1100-1106.Anonim (2017a) Pamuk [http:// www.bahçe. Biz/bitki/tarla/endüstri/ pamuk. htm] Erişim Tarihi: 05.04.2018.Anonim (2018) https://agrilifecdn.tamu.edu/lubbock/files/2011/11/Integrated Management Verticillium Wilt Cotton.pdfAnagnostakis SL, Jaynes RA (1973) Chestnut blight control: use of hypovirulent cultures. Plant Dis. Rep. 57:225-226.Akıllı S, Ulubaş-Serçe Ç, Katırcıoğlu YZ, Maden S, Rigling D (2012) Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey. European Journal of Plant Pathology 135(2): 323-334. Biçiçi M, Kurt Ş (1998) Etiology, İncidence and Prevalence of Cotton Wilt Disease and Strains of The Wilt Pathogen in Çukurova. World Cotton Research Conference. 6-12 September, Athens, GreeceBalijja A, Kvarnheden A, Turchett, T (2008) A non-phenol–chloro form extraction of double-stranded RNA fromplantandfungaltissues. Journal of Virological Methods 152: 32–37.Bissegger M, Rigling D, Heiniger U (1997) Population structure and disease development of Cryphonectria parasitica in European chestnut forests in the presence of natural hypovirulence. Phytopathology 87: 50–59.Bisiach M, De Martino A, Gobbi E, Intropido M, Vegetti G (1988) Studies on chestnut blight: activity report. Rivisa di Patalogia Vegetale S. IV, 24: 3-13.Canızares M., Pérez-artés E, García-pedrajas NE, García-pedrajas MD (2015) Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions, Phytopathologia Mediterranea 54, 3, 516−523.Cao Y-F, Zhu X-W, Xiang Y, Li D-G, Yang J-R, Mao Q-Z, Chen J-S (2011) Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb. Virus Research 159, 73–78.De Lange W J, Wingfield BD, Wingfield MJ 1(998) A rapid, apple- based test for virulence in Cryphonectria cubensis isolates. Eur. J. For. Phytopathology 28: 409- 412.Domsch KH, Gams W, Anderson TH (1980) Compendium of Soil Fungi, Volume I. Academic Press, 859 pp.Elliston JE (1985) Characteristics of dsRNA- containing strains of Endothia parasitica in relation to hypovirulence. Phytopathology 75: 151-158.El-Zik KM (1985) Integrated Control of Verticillium Wilt of Cotton. Plant Disease 1025-1032Erdoğan O, Sezener V, Özbek N, Bozbek T, Yavaş İ, Ünay A (2006) The effects of Verticillium wilt (Verticillium dahliae Kleb.) on cotton yieldand fiber quality. Asian Journal of Plant Science 5: 867-870.Esentepe M (1979) Adana ve Antalya illerinde pamuklarda görülen solgunluk hastalığının etmeni, yayılışı, kesafeti ve zarar derecesi ile ekolojisi üzerinde araştırmalar. İzmir Bölge Zirai Mücadele Araştırma Enstitüsü Müdürlüğü Araştırma Eserleri Serisi, Yayın No:32, Sayfa No: 45Feng Z, Zhu H, Shi Y, Zhao L, Liu L, Jiang D (2013) Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb. Annotated Sequence Record 158:2621–2623.Gencer O (1987.)Genel Tarla Bitkileri. Ç. Ü. Ziraat Fakültesi. Adana, 135s.Galanopoulo S (2006) http://www.ressources.ciheam.org./om/pdf/s14/CI01190Gencer O, Mert M, Kurt Ş (2001) Bazı Pamuk hat ve çeşitlerinin solgunluk hastalığına (V.dahliae Kleb.) tepkisi ile bunların tarımsal ve teknolojik özelliklerinin belirlenmesi. IV. Tarla Bitkileri Kongresi, 17-21 Eylül 2001, Tekirdağ, 193-197Godoy A, Palomo G, Garcıa C (1995) Performance of New Cotton Cultivars on Verticillium dahliae Kleb. Infested Soils at the Comarca Lagunera, Mexico, Proceedings Beltwide Cotton Conference 498-500, Tennis.Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe. Annu. Rev. Phytopathol 32:581-99.İyriboz N (1941) Mahsül Hastalıkları. Ziraat Vekaleti Neşriyatı Umum no:237.Karademir E, Karademir Ç, Ekinci R, Bars A, Çelik İ (2009) Solgunluk Hastalığı (V.dahliae Kleb.) Etmenine Karşı F5 Pamuk Hatlarının Reaksiyonlarının Belirlenmesi. Türkiye III. Bitki Koruma Kongresi 15-18 Temmuz 2009, Van. S, 179.Karaca İ, Karcılıoğlu A, Ceylan S (1971) Wilt Disease of Cotton in the Ege Region of Turkey. J.Turkish Phytopath 1:4-11.Agrios, 1997).Karaca İ (1974) Sistematik Bitki Hastalıkları. Deuteromycetes (FungiImperfecti), Cilt, IV. Ege Üniversitesi Ziraat Fakültesi Yayınları No:217. 148-159 s.Krstin L, Novak-Agbaba S, Rigling D, Krajacic M, CurkovicPerica M (2008) Chestnut blight fungus in Croatia: diversity of vegetative compatibility types, mating types and genetic variability of associated Cryphonectria hypovirus 1. Plant Pathology, 57:1086-1092.Lee JK, Tattar TA, Berman PM, Mount MS (1992) A rapid for testing the virulence of Cryphonectria parasitica using excised bark and wood of American chestnut. Phytopathology 82: 1454-1456.Melouk HA (1992) Methodsfor Research on Soil-Borne Phytopathogenic Fungi. ( L.L.Singleton, J.D. Mihall, C.M. Rusheds. Aps St. Paul) Verticillium. 175 -177 p. Macdonald WL, Fulbright DW (1991) The biological control of chestnut blight: use and limitations of transmissible hypovirulence. Plant Dis., 75: 656- 661.Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plantand fungaltissue. Phytopatology 69: 854–858.Moshırabadı H, Janlou M, Gajar A (2000) The Study of Verticillium Wilt In Preliminary Variety Trials and Common Variey Trials. The Interregional Cooperative Research Network on Cotton. A JointWorkshop and Meeting of the All Working Groups 20-24 September, 99-100, Adana-TURKEY.Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe.Annu.Rev. Phytopathol, 32:581-99.Morris TJ, Dodd JA (1979). Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopatology 69: 854–858.Nemli T (2003) Pamuk Hastalıkları ve Savaşım Yöntemleri. Pamukta Eğitim Semineri, 14-17 Ekim 2003, İzmir. S, 103-111.Pegg GF (1984) The impact of Verticillium diseases in agriculture. Phytopathol Mediterr 23:176-192.Robin C, Heiniger U (2001) Chestnut blight in Europe: Diversity of Cryphonectria parasitica, hypovirulence and biocontrol. Forest Snow and Landscape Research 76: 361–367Sağır A, Tatlı F (1995) Pamuk Solgunluk Hastalığı Etmeni (Verticillium dahliae Kleb)’ne Karşı Pamuk Çeşitlerinin Duyarlılıklarının Belirlenmesi Üzerinde Araştırmalar, VII. Türkiye Fitopatoloji Kongresi Bildirileri 26-29 Eylül 1995, Adana, 5-9.Sezgin E (1985) Pamuk Solgunluk Hastalığı ile Savaşımda Kültürel İşlemlerin Önemi. Yıllık 3 (3): 23-31, İzmir.Schnathorst WC, Cooper JR (1975) Anomalies in Field and Green house Reaction of Certain Cotton Cultivars in Fectedwith Verticillium dahliae. In Proc. Beltwide Cotton Prod. Conf., 6-8 January, New Orleans, National Cotton Council, Memphis, p. 148-149.Sotirovski K( 2000) Hypovirulence, Vegetative-Compatibility Groups and Mating Types of Cryphonectria parasitica (Murr.) Barr.in the Republic of Macedonia. PhD thesis. Skopje: University Ss. Kiril i Metodij.Töngüşlü M (2015) Ege Bölgesi Kiraz Alanlarında Leucostomaspp. İzolatlarının Virülenslik ve dsRNA Varlığı Yönünden İncelenmesi. Adnan Menderes Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı,55, Aydın. Türkiye.Trestic T, Uscuplic M, Colinas C, Rolland G, Giraud A, Robin C, (2001) Vegetative compatibility type diversity of Cryphonectria parasitica in Bosnia-Herzegovina, Spain and France. Forest Snow and Landscape Research, 76:391–396.Wilhelm S, Sagen JE, Tietz H (1974) Resistance to Verticillium Wilt in cotton: source, techniques of identification inheritance trends and resistance potential of Multipline Cultivars. Phytopathology 64:924-931

Pamuk Solgunluk Hastalık Etmeni Verticillium dahliae Kleb.’da Mikovirüs dsRNA Analizi ve RT-PCR İle Tanılanması

Year 2019, Volume: 29 Issue: 3, 516 - 525, 30.09.2019
https://doi.org/10.29133/yyutbd.496476

Abstract

Verticillium
dahliae
Kleb.
pamuk ve zeytin üretimi yapılan her yerde ciddi verim ve bitki kayıplarına
neden olan önemli bir fungal hastalıktır. Hastalığın toprak kökenli olması
mücadeleyi zorlaştırmaktadır. Fungal hastalıklarda biyolojik mücadele gün
geçtikçe ön plana çıkmakta olup biyolojik ajan olarak mikoviral dsRNA’lar da
kullanılmaya başlamıştır. Verticillium
dahliae
mikoviral dsRNA’lar ile ilgili ikisi pamukta biri zeytinde olmak
üzere toplamda dört çalışma yapılmıştır. Ancak Türkiye’de pamuk bitkisinde
solgunluk hastalığına neden olan V.
dahliae’
da mikovirüs dsRNA’nın varlığı hakkında bir çalışmaya
rastlanılmamıştır. Bu çalışmada Aydın ili Söke ve Koçarlı ilçelerinin pamuk
alanlarından solgunluk belirtisi gösteren 100 örnekten elde edilen 72 V. dahliae izolatında mikovirüs
dsRNA’nın varlığı araştırılmış ve on iki V.
dahliae
izolatında dsRNA belirlenmiştir. V.dahliae mikoviral dsRNA’nın RT-PCR ile tanılanması zeytindeki Verticillium dahliae partitivirus
1–olive (VdPV1-ol) mikovirüsü için tasarlanan spesifik primerler ve rastgele
hegzamer primeri kullanılarak yapılmıştır. Sonuçta kullandığımız bu primerler
ile pamuktaki V.dahliae izolatlarındaki
dsRNA mikovirüsünün tanılanamayacağı saptanmıştır. Çalışmada dsRNA içeren V. dahliae izolatlarının virülensliğini
belirlemek için elma testi uygulanmış ancak sonuç alınamamıştır.
 

References

  • Adams GC, Hammer SA (1989) Optimum sample size for detecting virulence in Leucostoma isolates from peach. Plant Disase 73: 754-759.Agrıos GN (1997) Plant Pathology. Academik Press. 635 p. Florida.Al-Rawahı AK, Hancock JG (1998( Parasitism and biological control of Verticillium dahliae by Pythium oligandrum. Plant Disease 82: 1100-1106.Anonim (2017a) Pamuk [http:// www.bahçe. Biz/bitki/tarla/endüstri/ pamuk. htm] Erişim Tarihi: 05.04.2018.Anonim (2018) https://agrilifecdn.tamu.edu/lubbock/files/2011/11/Integrated Management Verticillium Wilt Cotton.pdfAnagnostakis SL, Jaynes RA (1973) Chestnut blight control: use of hypovirulent cultures. Plant Dis. Rep. 57:225-226.Akıllı S, Ulubaş-Serçe Ç, Katırcıoğlu YZ, Maden S, Rigling D (2012) Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey. European Journal of Plant Pathology 135(2): 323-334. Biçiçi M, Kurt Ş (1998) Etiology, İncidence and Prevalence of Cotton Wilt Disease and Strains of The Wilt Pathogen in Çukurova. World Cotton Research Conference. 6-12 September, Athens, GreeceBalijja A, Kvarnheden A, Turchett, T (2008) A non-phenol–chloro form extraction of double-stranded RNA fromplantandfungaltissues. Journal of Virological Methods 152: 32–37.Bissegger M, Rigling D, Heiniger U (1997) Population structure and disease development of Cryphonectria parasitica in European chestnut forests in the presence of natural hypovirulence. Phytopathology 87: 50–59.Bisiach M, De Martino A, Gobbi E, Intropido M, Vegetti G (1988) Studies on chestnut blight: activity report. Rivisa di Patalogia Vegetale S. IV, 24: 3-13.Canızares M., Pérez-artés E, García-pedrajas NE, García-pedrajas MD (2015) Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions, Phytopathologia Mediterranea 54, 3, 516−523.Cao Y-F, Zhu X-W, Xiang Y, Li D-G, Yang J-R, Mao Q-Z, Chen J-S (2011) Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb. Virus Research 159, 73–78.De Lange W J, Wingfield BD, Wingfield MJ 1(998) A rapid, apple- based test for virulence in Cryphonectria cubensis isolates. Eur. J. For. Phytopathology 28: 409- 412.Domsch KH, Gams W, Anderson TH (1980) Compendium of Soil Fungi, Volume I. Academic Press, 859 pp.Elliston JE (1985) Characteristics of dsRNA- containing strains of Endothia parasitica in relation to hypovirulence. Phytopathology 75: 151-158.El-Zik KM (1985) Integrated Control of Verticillium Wilt of Cotton. Plant Disease 1025-1032Erdoğan O, Sezener V, Özbek N, Bozbek T, Yavaş İ, Ünay A (2006) The effects of Verticillium wilt (Verticillium dahliae Kleb.) on cotton yieldand fiber quality. Asian Journal of Plant Science 5: 867-870.Esentepe M (1979) Adana ve Antalya illerinde pamuklarda görülen solgunluk hastalığının etmeni, yayılışı, kesafeti ve zarar derecesi ile ekolojisi üzerinde araştırmalar. İzmir Bölge Zirai Mücadele Araştırma Enstitüsü Müdürlüğü Araştırma Eserleri Serisi, Yayın No:32, Sayfa No: 45Feng Z, Zhu H, Shi Y, Zhao L, Liu L, Jiang D (2013) Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb. Annotated Sequence Record 158:2621–2623.Gencer O (1987.)Genel Tarla Bitkileri. Ç. Ü. Ziraat Fakültesi. Adana, 135s.Galanopoulo S (2006) http://www.ressources.ciheam.org./om/pdf/s14/CI01190Gencer O, Mert M, Kurt Ş (2001) Bazı Pamuk hat ve çeşitlerinin solgunluk hastalığına (V.dahliae Kleb.) tepkisi ile bunların tarımsal ve teknolojik özelliklerinin belirlenmesi. IV. Tarla Bitkileri Kongresi, 17-21 Eylül 2001, Tekirdağ, 193-197Godoy A, Palomo G, Garcıa C (1995) Performance of New Cotton Cultivars on Verticillium dahliae Kleb. Infested Soils at the Comarca Lagunera, Mexico, Proceedings Beltwide Cotton Conference 498-500, Tennis.Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe. Annu. Rev. Phytopathol 32:581-99.İyriboz N (1941) Mahsül Hastalıkları. Ziraat Vekaleti Neşriyatı Umum no:237.Karademir E, Karademir Ç, Ekinci R, Bars A, Çelik İ (2009) Solgunluk Hastalığı (V.dahliae Kleb.) Etmenine Karşı F5 Pamuk Hatlarının Reaksiyonlarının Belirlenmesi. Türkiye III. Bitki Koruma Kongresi 15-18 Temmuz 2009, Van. S, 179.Karaca İ, Karcılıoğlu A, Ceylan S (1971) Wilt Disease of Cotton in the Ege Region of Turkey. J.Turkish Phytopath 1:4-11.Agrios, 1997).Karaca İ (1974) Sistematik Bitki Hastalıkları. Deuteromycetes (FungiImperfecti), Cilt, IV. Ege Üniversitesi Ziraat Fakültesi Yayınları No:217. 148-159 s.Krstin L, Novak-Agbaba S, Rigling D, Krajacic M, CurkovicPerica M (2008) Chestnut blight fungus in Croatia: diversity of vegetative compatibility types, mating types and genetic variability of associated Cryphonectria hypovirus 1. Plant Pathology, 57:1086-1092.Lee JK, Tattar TA, Berman PM, Mount MS (1992) A rapid for testing the virulence of Cryphonectria parasitica using excised bark and wood of American chestnut. Phytopathology 82: 1454-1456.Melouk HA (1992) Methodsfor Research on Soil-Borne Phytopathogenic Fungi. ( L.L.Singleton, J.D. Mihall, C.M. Rusheds. Aps St. Paul) Verticillium. 175 -177 p. Macdonald WL, Fulbright DW (1991) The biological control of chestnut blight: use and limitations of transmissible hypovirulence. Plant Dis., 75: 656- 661.Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plantand fungaltissue. Phytopatology 69: 854–858.Moshırabadı H, Janlou M, Gajar A (2000) The Study of Verticillium Wilt In Preliminary Variety Trials and Common Variey Trials. The Interregional Cooperative Research Network on Cotton. A JointWorkshop and Meeting of the All Working Groups 20-24 September, 99-100, Adana-TURKEY.Heiniger U, Rigling D (1994) Biological control of chestnut blight in Europe.Annu.Rev. Phytopathol, 32:581-99.Morris TJ, Dodd JA (1979). Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopatology 69: 854–858.Nemli T (2003) Pamuk Hastalıkları ve Savaşım Yöntemleri. Pamukta Eğitim Semineri, 14-17 Ekim 2003, İzmir. S, 103-111.Pegg GF (1984) The impact of Verticillium diseases in agriculture. Phytopathol Mediterr 23:176-192.Robin C, Heiniger U (2001) Chestnut blight in Europe: Diversity of Cryphonectria parasitica, hypovirulence and biocontrol. Forest Snow and Landscape Research 76: 361–367Sağır A, Tatlı F (1995) Pamuk Solgunluk Hastalığı Etmeni (Verticillium dahliae Kleb)’ne Karşı Pamuk Çeşitlerinin Duyarlılıklarının Belirlenmesi Üzerinde Araştırmalar, VII. Türkiye Fitopatoloji Kongresi Bildirileri 26-29 Eylül 1995, Adana, 5-9.Sezgin E (1985) Pamuk Solgunluk Hastalığı ile Savaşımda Kültürel İşlemlerin Önemi. Yıllık 3 (3): 23-31, İzmir.Schnathorst WC, Cooper JR (1975) Anomalies in Field and Green house Reaction of Certain Cotton Cultivars in Fectedwith Verticillium dahliae. In Proc. Beltwide Cotton Prod. Conf., 6-8 January, New Orleans, National Cotton Council, Memphis, p. 148-149.Sotirovski K( 2000) Hypovirulence, Vegetative-Compatibility Groups and Mating Types of Cryphonectria parasitica (Murr.) Barr.in the Republic of Macedonia. PhD thesis. Skopje: University Ss. Kiril i Metodij.Töngüşlü M (2015) Ege Bölgesi Kiraz Alanlarında Leucostomaspp. İzolatlarının Virülenslik ve dsRNA Varlığı Yönünden İncelenmesi. Adnan Menderes Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı,55, Aydın. Türkiye.Trestic T, Uscuplic M, Colinas C, Rolland G, Giraud A, Robin C, (2001) Vegetative compatibility type diversity of Cryphonectria parasitica in Bosnia-Herzegovina, Spain and France. Forest Snow and Landscape Research, 76:391–396.Wilhelm S, Sagen JE, Tietz H (1974) Resistance to Verticillium Wilt in cotton: source, techniques of identification inheritance trends and resistance potential of Multipline Cultivars. Phytopathology 64:924-931
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Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Deniz Kübra Balcı 0000-0002-2301-3897

Serap Açıkgöz 0000-0002-7970-1648

Publication Date September 30, 2019
Acceptance Date August 19, 2019
Published in Issue Year 2019 Volume: 29 Issue: 3

Cite

APA Balcı, D. K., & Açıkgöz, S. (2019). Pamuk Solgunluk Hastalık Etmeni Verticillium dahliae Kleb.’da Mikovirüs dsRNA Analizi ve RT-PCR İle Tanılanması. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(3), 516-525. https://doi.org/10.29133/yyutbd.496476
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