Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

Nevzat Sevgin; Aveen Khorsheed Khalid; Merve Özkul

doi:10.20289/zfdergi.1198529

EN TR

Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

Abstract

Objective: The purpose of this research was to investigate the effects of charcoal and physical state of culture medium on growth of Morus nigra. L. Material and Methods: For the study tree mature black mulbery (M. nigra) genotypes A5, T6 and T8 grown in vitro were used as the explant source in this study. To find out the possible effects of charcoal and culture physical state (culture medium) on culture growth used four) Liquid Nas and Read Medium (NRM) + 0.5 gl-1 activated charcoal, Liquid NRM containing no charcoal, Gelled NRM + 0.5 gl-1 activated charcoal, agar-gelled NRM containing no charcoal) medium were used in the study to find out the possible effects of the presence of charcoal and physical state of the culture medium on culture growth. Results: Medium physical state and presence of activated charcoal in the medium greatly affected mean shoot number per cultured explants. Mean shoot numbers obtained per explants varied between 1.1 and 5.8. Medium containing no charcoal produced more shoots compared to medium containing charcoal and agar - gelled medium produced more shoots compared to liquid medium. Conclusion: Agar- The gelled medium with agar gave better results for black mulbery (M. nigra) than the liquid medium.

Keywords

Activated charcoal , in vitro , gelled medium , liquid medium , Morus nigra L.

Aktif karbon ve kültür ortamının fiziksel halinin Urmu dutu (Morus nigra L.) nun mikroçoğaltımı üzerine etkisi

Öz

Amaç: Bu çalışmanın amacı Morus nigra L. nin in vitro mikro çoğaltımı üzerinde aktif kömür ve kültür ortamının fiziksel halinin araştırılmasıdır. Materyal ve Yöntem: Çalışmada Urmu dutun (M. nigra) 3 genotipi (A5, T6, T8) explant olarak kullanılmıştır. Kültür ortamında kömürün varlığı ve ortamın fiziksel halinin (sıvı veya jel) incelenmesi için sıvı Nas ve Read medium (NRM)+0,5 gr L-1 aktif kömür, aktif kömürsüz sıvı NRM ortamı, katı NRM ortamı+0,5 gL-1 aktif kömür ve aktif kömürsüz NRM ortamı kullanılmıştır. Araştırma Bulguları: Kültür ortamında kömürün varlığı ve ortamın fiziksel hali (sıvı veya jel) bir eksplantten elde edilen ortalama sürgün sayısını önemli derecede etkilemiştir. Bir eksplantten elde edilen ortalama sürgün sayısı 1.1 ile 5.8 arasında olmuştur. Aktif kömür içermeyen ortam üzerinde daha fazla sürgün elde edilmiş ve sıvı ortama kıyasla jelleştirilmiş ortam üzerinde elde edilen sürgün sayısı daha yüksek olmuştur. Sonuç: Urmu dutunun İn vitro mikroçoğaltımında agar ile katılaştırılmış NRM ortamı sıvı NRM ortamından daha iyi sonuç vermiştir.

Anahtar Kelimeler

Aktif kömür , in vitro katı ortam , sıvı ortam , Morus nigra L.

References

Adelderg, J., M. Koroggel & J. Toler, 2000. Physical environment in vitro affects laboratory and nursery growth of micropropagated Hostas. HortTechnology, 10 (4): 754-757. https://doi.org/10.21273/HORTTECH.10.4.754
Altman, A., 2000. “Micropropagation of Plants, Principles and Practice, 916-929”. In: Encyclopedia of Cell Technology (Ed. R. E. Spier). NewYork: John Wiley & Sons, 1249 pp.
Bae, S.H. & H.J. Suh, 2007. Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Science and Technology, 40 (6): 955-962. https://doi.org/10.1016/j.lwt.2006.06.007
Baytop, T., 1996. Türkiye‟de Bitkiler ile Tedavi. I.U. Yayinlari No: 3255, Eczacilik Fak., 40:444. Baytop, T., 1999. Türkiye’de Bitkiler ile Tedavi. Nobel Tıp Kitabevleri, İstanbul. 409s.
Bhau, B. S & A. K. Wakhlu, 2001. Effect of genotype, explant type and growth regulators on organogenesis in (Morus alba L.). Plant Cell Tissue Organ Culture, 66: 25-29. https://doi.org/10.1023/A:1010617212237
Bhau, B.S. & A.K. Wakhlu, 2003. Rapid micropropagation of five cultivars of mulberry. Biologia Plantarum, 46 (3): 349-355. https://doi.org/10.1023/A:1024313832737
Chandra, S., R. Bandopadhyay, V. Kumar & R. Chandra, 2010. Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnology letters, 32 (9): 1199-1205. https://doi.org/10.1007/s10529-010-0290-0
Chen, J., J. Kan, J. Tang, Z. Cai & J. Liu, 2012. The profile in polyphenols and volatile compounds in alcoholic beverages from different cultivars of mulberry. Journal of Food Science, 77 (4): C430-C436. https://doi.org/10.1111/j.1750-3841.2011.02593.x
Chitra, D. S. V. & G. Padmaja, 1999. Clonal propagation of mulberry (Morus indica L. cultivar M-5) through in vitro culture of nodal explants. Scientia Horticulturae, 80 (3-4): 289-298. https://doi.org/10.1016/S0304-4238 (98)00252-0
Dairas, M. J., G.R. Lobo & J.C. Hernandez, 2003. Alcoholic beverages obtained from black mulberry. Food Technology and Biotechnology, 41 (2): 173-176.

Das, B. C., 1983. Mulberry taxonomy, cytogenetics and breeding. In: National Seminar on Silk Research and Development (Vol. 10, p. 135).
Doğan, M., 2022. In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium. Current Perspectives on Medicinal and Aromatic Plants (CUPMAP), 5 (1): 12-18. https://doi.org/10.38093/cupmap.1057290
Ercisli, S. & E. Orhan, 2007. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food chemistry, 103 (4): 1380-1384. https://doi.org/10.1016/j.foodchem.2006.10.054
Gerasopoulos, D. & G. Stavroulakis, 1997. Quality characteristics of four mulberry (Morus spp.) cultivars in the area of Chania Greece. Journal of the Science of Food and Agriculture, 73 (2): 261-264. https://doi.org/10.1002/ (SICI)1097-0010 (199702) 73:2<261::AID-JSFA724>3.0.CO;2-S
Hepaksoy, S., 2017. GF 677 (P. amygdalus x P. persica) klon anacının doku kültüründe sürgünucu tekniği ile çoğaltılması. Journal of Agriculture Faculty of Ege University, 54 (4): 447-451.
Hossain, M., S.M. Rahman, A. Zaman, O.I. Joarder & R. Islam, 1992. Micropropagation of Morus laevigata Wall. from mature trees. Plant Cell Reports, 11 (10): 522-524. https://doi.org/10.1007/BF00236269
Kapur, A., S. Bhatnagar & P. Khurana, 2001. Efficient regeneration from mature leaf explants of Indian mulberry via organogenesis. Sericologia (France), 41: 207-214.
Lalitha, N., S. Kih, R. Banerjee, S. Chattopadhya, A.K. Saha & B.B. Bindroo, 2013. High frequency multiple shoot induction and in vitro regeneration of mulberry (Morus indica L. cv. S-1635). International Journal of Advanced Research, 1: 22-26.
Lu, M., 2002. Micropropagation of Morus latifolia poilet using axillary buds from mature trees. Scientia Horticulturae, 96 (1-4): 329-341. https://doi.org/10.1016/S0304-4238 (02)00120-6
Nas, M. N. & P.E. Read, 2004. A hypothesis for the development of a defined tissue cultu- re medium of higher plants and micropropagation of hazelnuts. Scientia Horticulturae, 101 (1-2): 189-200. https://doi.org/10.1016/j.scienta.2003.10.004
Nas, M. N., L. Gokbunar, N. Sevgin, M. Aydemir, M. Dagli & Z. Susluoglu, 2012. Micropropagation of mature Crataegus aronia L., a medicinal and ornamental plant with rootstock potential for pome fruit. Plant Growth Regulation, 67 (1): 57-63. https://doi.org/10.1007/s10725-012-9662-x
Ohyama, K. & S. Oka, 1987. "Mulberry." Cell and Tissue Culture in Forestry. Springer, Dordrecht, 1987. 272-284. https://doi.org/10.1007/978-94-017-0992-7_20
Ouyang, Z., J. Chen & Y.H. Li, 2005. Separation, purification and composition analysis of polysaccharides in leaves of Morus alba L. Food Science (China), 26 (3): 181-184.
Pati, P. K., S.P. Rath, M. Sharma, A. Sood & P.S. Ahuja, 2006. In vitro propagation of rose: a review. Biotechnolgy Advances, 24: 94-114. https://doi.org/10.1016/j.biotechadv.2005.07.001
Rajore, S. & A. Batra, 2005. Efficient plant regeneration via shoot tip explant in Jatrophacurcas L. Journal of Plant Biochemistry and Biotechnology, 14 (1): 73-75. https://doi.org/10.1007/BF03263231
Vijayan, K., B. Saratchandra, D.A. Teixeira & J.A. Silva, 2011. Germplasm conservation in mulberry (Morus spp.). Scientia Horticulturae, 128 (4): 371-379. https://doi.org/10.1016/j.scienta.2010.11.012
Wang, R. S., P.H. Dong, X.X. Shuai & M.S. Chen, 2022. Evaluation of different black mulberry fruits (Morus nigra L.) based on phenolic compounds and antioxidant activity. Foods, 11 (9). https://doi.org/10.3390/foods11091252.
Wasano, N., K. Konno, M. Nakamura, C. Hirayama, M. Hattori & K. Tateishi, 2009. A unique latex protein, MLX56, defends mulberry trees from insects. Phytochemistry, 70 (7): 880-888. https://doi.org/10.1016/j.phytochem.2009.04.014
Zainel, A. A. & S. Hepaksoy, 2018. Bir idris anacı ‘pontaleb’in doku kültürü ile çoğaltılma olanaklarının araştırılması. Journal of Agriculture Faculty of Ege University, 55 (1): 83-88. https://doi.org/10.20289/zfdergi.390987
Zaman, A., R. Islam & O.I. Joarder, 1997. Field performance and biochemical evaluation of micropropagated mulberry plants. Plant Cell, Tissue and Organ Culture, 51 (1): 61-64. https://doi.org/10.1023/A:1005830515585

Details

Primary Language

English

Subjects

Agricultural Engineering

Journal Section

Research Article

Authors

Nevzat Sevgin ^*
0000-0001-5405-060X
Türkiye

Aveen Khorsheed Khalid
0000-0003-0544-8720
Iraq

Merve Özkul
0000-0002-1769-6847
Türkiye

Publication Date

April 1, 2023

Submission Date

November 8, 2022

Acceptance Date

February 3, 2023

Published in Issue

Year 2023 Volume: 60 Number: 1

DOI

https://doi.org/10.20289/zfdergi.1198529

IZ

https://izlik.org/JA76YZ59AT

APA

Sevgin, N., Khalid, A. K., & Özkul, M. (2023). Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.). Journal of Agriculture Faculty of Ege University, 60(1), 53-60. https://doi.org/10.20289/zfdergi.1198529

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