The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease

Sercan Pazarlar; Eray Şimşek

doi:10.20289/zfdergi.1461733

EN TR

The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease

Abstract

Objective: The objective of this study is to assess the efficacy of Serendipita indica, a basidiomycete endophyte, as a biological control agent against Fusarium wilt disease caused by Fusarium oxysporum f. sp. ciceris in disease susceptible chickpea cultivar JG62. Material and Methods: Chlamydospores of Serendipita indica were applied to the roots of the germinated JG62 variety, followed by inoculation with Fusarium oxysporum f. sp. ciceris race 5 one week after the application. Disease severity and plant fresh weight were measured 25 days after inoculation. The colonization pattern of Serendipita indica and Fusarium oxysporum f. sp. ciceris were monitored by quantifying fungal DNA using qPCR over time. The antagonistic interactions between the fungi were determined using the dual-culture method. Results: Serendipita indica successfully colonized the chickpea roots leading to a decrease in biomass of Fusarium oxysporum f. sp. ciceris in the roots and diminished the overall symptoms such as wilting and yellowing caused by Fusarium infection. Besides, direct antagonistic effect of Serendipita indica was found against Fusarium in vivo conditions. Conclusion: These results indicate the potential of Serendipita indica as a biological control agent in developing sustainable strategies for managing Fusarium wilt of chickpea.

Keywords

Biocontrol agents, fungal endophyte, Fusarium oxysporum f. sp. ciceris, plant defense, Serendipita indica

Endofitik Fungus Serendipita indica kolonizasyonu nohut bitkilerini Fusarium solgunluğu hastalığına karşı korumaktadır

Abstract

Amaç: Bu çalışmanın amacı, nohutlarda Fusarium oxysporum f. sp. ciceris tarafından kaynaklanan Fusarium solgunluğu hastalığına karşı biyolojik kontrol ajanı olarak Serendipita indica adlı bir basidiomycete endofit fungusun etkinliğini değerlendirmektir. Materyal ve Yöntem: Serendipita indica klamidosporları çimlendirilen JG62 çeşidinin köklerine uygulanmış ve uygulamadan 1 hafta sonra Fusarium oxysporum f. sp. ciceris ırk 5 inokulasyonu yapılmıştır. Hastalık şiddeti ve bitki yaş ağırlığı inokulasyondan 25 gün sonra ölçülmüştür., Serendipita indica ve Fusarium oxysporum f. sp. ciceris kolonizasyonu fungal DNA miktarının zamana bağlı olarak qPCR ile belirlenmesiyle izlenmiştir. Funguslar arasındaki antagonistik etkileşimler ikili-kültür yöntemi kullanılarak belirlenmiştir. Araştırma Bulguları: Serendipita indica başarılı bir şekilde nohut köklerini işgal ederek köklerdeki Fusarium oxysporum f. sp. ciceris biyomasının azalmasına ve Fusarium enfeksiyonu tarafından neden olan solgunluk ve sararma gibi belirtilerin azalmasına neden olmuştur. Ayrıca, Serendipita indica'nın Fusarium solgunluğu etmenine karşı doğrudan antagonistik etkisi bulunmuştur. Sonuç: Bu sonuçlar, nohutta Fusarium solgunluğuna karşı sürdürülebilir stratejiler geliştirmede Serendipita indica'nın biyolojik kontrol ajanı olarak potansiyelini göstermektedir.

Keywords

Biyolojik kontrol, fungal endofit, Fusarium oxysporum f.sp. ciceris, bitki savunması ajanı, Serendipita indica

Thanks

The authors would like to thank Assoc. Prof. Dr. Abdullah Kahraman (Harran University, Türkiye) for providing Fusarium disease susceptible chickpea variety and Prof. Dr. Canan Can (Gaziantep University, Türkiye) for providing Fusarium oxysporum f.sp. ciceris (race 5) isolate. The authors also thank Dr. Jafargholi Imani (Justus Liebig University Giessen, Research Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology, Giessen, Germany) for providing S. indica strain.

References

Akbaba, M. & T. Genc, 2024. Domateste toprak kökenli fungal patojenlerin mücadelesinde rizobakterilerin kullanılma potansiyeli. Ege Üniversitesi Ziraat Fakültesi Dergisi, 60 (4): 705-721. https://doi.org/10.20289/zfdergi.1342790
Aslam, M.M., J. Karanja & S.K. Bello, 2019. Piriformospora indica colonization reprograms plants to improved P-uptake, enhanced crop performance, and biotic/abiotic stress tolerance. Physiological and Molecular Plant Pathology, 106: 232-237. https://doi.org/10.1016/j.pmpp.2019.02.010
Bhar, A., A. Jain & S. Das, 2021. Soil pathogen, Fusarium oxysporum induced wilt disease in chickpea: A review on its dynamicity and possible control strategies. Proceedings of the Indian National Science Academy, 87 (2): 260-274. https://doi.org/10.1007/s43538-021-00030-9
Bharadwaj, C., J. Jorben, A. Rao, M. Roorkiwal, V. Jayalakshmi, A. Chitikineni, S. Singh, I. Singh, A. Pratap, G. P. Dixit, A. K. Srivastava & R. K. Varshney, 2022. Development of high yielding fusarium wilt resistant cultivar by pyramiding of “genes” through marker-assisted backcrossing in chickpea (Cicer arietinum L.). Frontiers in Genetics, 13: 924287. https://doi.org/10.3389/fgene.2022.924287
Boorboori, M. R. & H.Y. Zhang, 2022. The role of Serendipita indica (Piriformospora indica) in improving plant resistance to drought and salinity stresses. Biology, 11 (7): 952. https://doi.org/10.3390/biology11070952
Bubici, G., M. Kaushal, M.I. Prigigallo, C. Gómez-Lama Cabanás & J. Mercado-Blanco, 2019. Biological control agents against fusarium wilt of banana. Frontiers in Microbiology, 10: 616. https://doi.org/10.3389/fmicb.2019.00616
Chakraborty, J., S. Sen, P. Ghosh, A. Jain & S. Das, 2020. Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition. BMC Plant Biology, 20: 319. https://doi.org/10.1186/s12870-020-02527-9
Chaudhary, P., U. Agri, A. Chaudhary, A. Kumar & G. Kumar, 2022. Endophytes and their potential in biotic stress management and crop production. Frontiers in Microbiology, 13: 933017. https://doi.org/10.3389/fmicb.2022.933017
Cheng, C., D. Li, Q. Qi, X. Sun, M.R. Anue, B.M. David, Y. Zhang, X. Hao, Z. Zhang & Z. Lai, 2020. The root endophytic fungus Serendipita indica improves resistance of Banana to Fusarium oxysporum f. sp. cubense tropical race 4. European Journal of Plant Pathology, 156 (1): 87-100. https://doi.org/10.1007/s10658-019-01863-3
Daneshkhah, R., F.M.W. Grundler & K. Wieczorek 2018. The role of MPK6 as mediator of ethylene/jasmonic acid signaling in Serendipita indica-colonized arabidopsis roots. Plant Molecular Biology Reporter, 36 (2): 284-294. https://doi.org/10.1007/s11105-018-1077-z

del Barrio-Duque, A., J. Ley, A. Samad, L. Antonielli, A. Sessitsch & S. Compant, 2019. Beneficial endophytic bacteria-Serendipita indica interaction for crop enhancement and resistance to phytopathogens. Frontiers in Microbiology, 10: 2888. https://doi.org/10.3389/FMICB.2019.02888
Deshmukh, S. D. & K.H. Kogel, 2007. Piriformospora indica protects barley from root rot caused by Fusarium graminearum. Journal of Plant Diseases and Protection, 114 (6): 263-268.
Deshmukh, S., R. Hückelhoven, P. Schäfer, J. Imani, M. Sharma, M. Weiss, F. Waller & K.H. Kogel, 2006. The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley. Proceedings of the National Academy of Sciences, 103 (49): 18450-18457. https://doi.org/10.1073/pnas.0605697103
Doyle, J.J. & J.L. Doyle, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19 (1): 11-15.
Dubey, S.C., M. Suresh & B. Singh, 2007. Evaluation of Trichoderma species against Fusarium oxysporum f. sp. Ciceris for integrated management of chickpea wilt. Biological Control, 40 (1): 118-127. https://doi.org/10.1016/j.biocontrol.2006.06.006
Felle, H.H., F. Waller, A. Molitor & K.H. Kogel, 2009. The mycorrhiza fungus Piriformospora indica induces fast root-surface ph signaling and primes systemic alkalinization of the leaf apoplast upon powdery mildew infection. Molecular Plant-Microbe Interactions, 22 (9): 1179-1185. https://doi.org/10.1094/MPMI-22-9-1179
Fontana, D.C., S. de Paula, A.G. Torres, V.H.M. de Souza, S.F. Pascholati, D. Schmidt & D. Dourado Neto, 2021. Endophytic fungi: biological control and induced resistance to phytopathogens and abiotic stresses. Pathogens, 10 (5): 570. https://doi.org/10.3390/pathogens10050570
Ghezel Sefloo, N., K. Wieczorek, S. Steinkellner & K. Hage-Ahmed, 2019. Serendipita species trigger cultivar-specific responses to fusarium wilt in tomato. Agronomy, 9 (10): 595. https://doi.org/10.3390/agronomy9100595
Hallasgo, A.M., C. Hauser, S. Steinkellner & K. Hage-Ahmed, 2022. Single and coinoculation of Serendipita herbamans with arbuscular mycorrhizal fungi reduces Fusarium wilt in tomato and slows disease progression in the long-term. Biological Control, 168 (2022): 104876. https://doi.org/10.1016/j.biocontrol.2022.104876
Harrach, B.D., H. Baltruschat, B. Barna, J. Fodor & K.H. Kogel, 2013. The mutualistic fungus Piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen Fusarium culmorum. Molecular Plant-Microbe Interactions, 26 (5): 599-605. https://doi.org/10.1094/MPMI-09-12-0216-R
Haware, M. P., Y.L. Nene & M. Natarajan, 1996. The survival of Fusarium oxysporum f. sp. ciceri in the soil in the absence of chickpea. Phytopathologia Mediterranea, 35 (1): 9-12.
Huang, Y., J. Li, C. Nong, T. Lin, L. Fang, X. Feng, Y. Chen, Y. Lin, Z. Lai & L. Miao, 2024. Piriformospora indica enhances resistance to fusarium wilt in strawberry by increasing the activity of superoxide dismutase, peroxidase, and catalase, while reducing the content of malondialdehyde in the roots. Horticulturae, 10 (3): 240. https://doi.org/10.3390/horticulturae10030240
Jacobs, S., B. Zechmann, A. Molitor, M. Trujillo, E. Petutschnig, V. Lipka, K.H. Kogel & P. Schäfer, 2011. Broad-spectrum suppression of innate immunity is required for colonization of arabidopsis roots by the fungus Piriformospora indica. Plant Physiology, 156 (2): 726-740. https://doi.org/10.1104/PP.111.176446
Jamil, A. & S. Ashraf, 2020. Utilization of chemical fungicides in managing the wilt disease of chickpea caused by Fusarium oxysporum f. sp. ciceri. Archives of Phytopathology and Plant Protection, 53 (17-18): 876-898. https://doi.org/10.1080/03235408.2020.1803705
Jendoubi, W., M. Bouhadida, A. Boukteb, M. Béji & M. Kharrat, 2017. Fusarium wilt affecting chickpea crop. Agriculture, 7 (3): 23. https://doi.org/10.3390/AGRICULTURE7030023
Jiménez-Díaz, R.M., P. Castillo, M. del M. Jiménez-Gasco, B.B. Landa & J. A. Navas-Cortés, 2015. Fusarium wilt of chickpeas: Biology, ecology and management. Crop Protection, 73 (February): 16-27. https://doi.org/10.1016/j.cropro.2015.02.023
Kumar, Y., B.B. Dholakia, P. Panigrahi, N.Y. Kadoo, A.P. Giri & V. S. Gupta, 2015. Metabolic profiling of chickpea-Fusarium interaction identifies differential modulation of disease resistance pathways. Phytochemistry, 116, 120-129. https://doi.org/10.1016/j.phytochem.2015.04.001
Kumari, S. & V. Khanna, 2020. Induction of systemic resistance in chickpea (Cicer arietinum L.) against Fusarium oxysporum f. sp. ciceris by antagonistic rhizobacteria in assistance with native Mesorhizobium. Current Microbiology, 77 (1): 85-98. https://doi.org/10.1007/s00284-019-01805-6
Landa, B.B., J.A. Navas-Cortés & R.M. Jiménez-Díaz, 2007. Integrated management of fusarium wilt of chickpea with sowing date. Host Resistance, and Biological Control, 94 (9): 946-960. https://doi.org/10.1094/PHYTO.2004.94.9.946
Li, H., M. Rodda, A. Gnanasambandam, M. Aftab, R. Redden, K. Hobson, G. Rosewarne, M. Materne, S. Kaur & A. T. Slater, 2015. Breeding for biotic stress resistance in chickpea: Progress and prospects. Euphytica, 204 (2): 257-288. https://doi.org/10.1007/s10681-015-1462-8
Li, L. & N. Guo, 2022. Effect of Piriformospora indica-induced systemic resistance and basal immunity against Rhizoctonia cerealis and Fusarium graminearum in wheat. Frontiers in Plant Science, 13: 836940. https://doi.org/10.3389/fpls.2022.836940
Lu, H., T. Wei, H. Lou, X. Shu & Q. Chen, 2021. A critical review on communication mechanism within plant-endophytic fungi interactions to cope with biotic and abiotic stresses. Journal of Fungi, 7: 719. https://doi.org/10.3390/jof7090719
Mansotra, P., P. Sharma & S. Sharma, 2015. Bioaugmentation of Mesorhizobium cicer, Pseudomonas spp. and Piriformospora indica for sustainable chickpea production. Physiology and Molecular Biology of Plants, 21 (3): 385-393. https://doi.org/10.1007/s12298-015-0296-0
Meena, K.K., S. Mesapogu, M. Kumar, M.S. Yandigeri, G. Singh & A.K. Saxena, 2009. Co-inoculation of the endophytic fungus Piriformospora indica with the phosphate-solubilising bacterium Pseudomonas striata affects population dynamics and plant growth in chickpea. Biology and Fertility of Soils, 46 (2): 169-174.
Narayan, O.P., N. Verma, A.K. Singh, R. Oelmüller, M. Kumar, D. Prasad, R. Kapoor, M. Dua & A.K. Johri, 2017. Antioxidant enzymes in chickpea colonized by Piriformospora indica participate in defense against the pathogen Botrytis cinerea. Scientific Reports, 7: 13553. https://doi.org/10.1038/s41598-017-12944-w
Nassimi, Z. & P. Taheri, 2017. Endophytic fungus Piriformospora indica induced systemic resistance against rice sheath blight via affecting hydrogen peroxide and antioxidants. Biocontrol Science and Technology, 27 (2): 252-267. https://doi.org/10.1080/09583157.2016.1277690
Navas-Cortés, J.A., B. Hau & R.M. Jiménez-Díaz, 1998. Effect of sowing date, host cultivar, and race of Fusarium oxysporum f. sp. ciceris on development of fusarium wilt of chickpea. Phytopathology, 88 (12): 1338-1346. https://doi.org/10.1094/PHYTO.1998.88.12.1338
Navas-Cortés, J.A., B. Hau & R.M. Jiménez-Díaz, 2000. Yield loss in chickpeas in relation to development of fusarium wilt epidemics. Phytopathology, 90 (11): 1269-1278.
Nizam, S., X. Qiang, S. Wawra, R. Nostadt, F. Getzke, F. Schwanke, I. Dreyer, G. Langen & A. Zuccaro, 2019. Serendipita indica E5′NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization. EMBO Reports, 20 (2): e47430. https://doi.org/10.15252/embr.201847430
Noori, F., K. Rahnama, K. Mashayekhi, N. Akbari Oghaz, S.A. Movahedi Naeini & S. Hatamzadeh, 2023. Investigation of the symbiotic relationship between Serendipita indica and ZP684 hybrid cultivar of maize plant under a combined condition of root rot disease (Fusarium proliferatum) and drought stress. Biological Control, 184: 105282. https://doi.org/10.1016/j.biocontrol.2023.105282
Phurailatpam, L. & S. Mishra, 2020. “Role of Plant Endophytes in Conferring Abiotic Stress Tolerance, 603-628”. In: Plant Ecophysiology and Adaptation under Climate Change: Mechanisms and Perspectives II: Mechanisms of Adaptation and Stress Amelioration (Eds. M. Hasanuzzaman), Springer, 861 pp. https://doi.org/10.1007/978-981-15-2172-0_22
Pieterse, C.M.J., C. Zamioudis, R.L. Berendsen, D.M. Weller, S.C.M.V. Wees & P.A.H.M. Bakker, 2014. Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology, 52: 347-375. https://doi.org/10.1146/annurev-phyto-082712-102340
Poveda, J., 2021. Biological control of Fusarium oxysporum f. sp. ciceri and Ascochyta rabiei infecting protected geographical indication fuentesaúco-chickpea by Trichoderma species. European Journal of Plant Pathology, 160 (4): 825-840. https://doi.org/10.1007/s10658-021-02286-9
Qiang, X., M. Weiss, K.H. Kogel & P. Schäfer, 2012. Piriformospora indica-A mutualistic basidiomycete with an exceptionally large plant host range. Molecular Plant Pathology, 13 (5): 508-518. https://doi.org/10.1111/j.1364-3703.2011.00764.x
Rabiey, M., I. Ullah & M.W. Shaw, 2015. The endophytic fungus Piriformospora indica protects wheat from fusarium crown rot disease in simulated UK autumn conditions. Plant Pathology, 64 (5): 1029-1040. https://doi.org/10.1111/PPA.12335
Roylawar, P., K. Khandagale, S. Nanda, P.S. Soumia & S. Gawande, 2023. Colonization of Serendipita indica promotes resistance against Spodoptera exigua in onion (Allium cepa L.). Frontiers in Microbiology, 14: 1190942. https://doi.org/10.3389/fmicb.2023.1190942
Saleem, S., A. Sekara & R. Pokluda, 2022. Serendipita indica-A review from agricultural point of view. Plants, 11 (24): 3417. https://doi.org/10.3390/plants11243417
Schäfer, P., S. Pfiffi, L.M. Voll, D. Zajic, P.M. Chandler, F. Waller, U. Scholz, J. Pons-Kühnemann, S. Sonnewald, U. Sonnewald & K.H. Kogel, 2009. Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica. The Plant Journal, 59 (3): 461-474. https://doi.org/10.1111/j.1365-313X.2009.03887.x
Sharma, K.D., W. Chen & F.J. Muehlbauer, 2005. Genetics of chickpea resistance to five races of fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Disease, 89 (4): 385-390. https://doi.org/10.1094/PD-89-0385
Singhal, U., R. Prasad & A.Varma, 2017. “Piriformospora indica (Serendipita indica): The Novel Symbiont, 349-364”. In: Mycorrhiza-Function, Diversity, State of the Art (Eds. A. Varma, R. Prasad & N. Tuteja), Springer International Publishing, 396 pp. https://doi.org/10.1007/978-3-319-53064-2_17
Soltanzadeh, M., M. Soltani Nejad & G.H. Shahidi Bonjar, 2016. Application of soil-borne actinomycetes for biological control against Fusarium wilt of chickpea (Cicer arietinum) caused by Fusarium solani f. sp pisi. Journal of Phytopathology, 164 (11-12): 967-978. https://doi.org/10.1111/jph.12517
Stein, E., A. Molitor, K.H. Kogel & F. Waller, 2008. Systemic resistance in arabidopsis conferred by the mycorrhizal fungus Piriformospora indica requires jasmonic acid signaling and the cytoplasmic function of NPR1. Plant and Cell Physiology, 49 (11): 1747-1751. https://doi.org/10.1093/pcp/pcn147
Sun, C., Y. Shao, K. Vahabi, J. Lu, S. Bhattacharya, S. Dong, K.W. Yeh, I. Sherameti, B. Lou, I. T. Baldwin & R. Oelmüller, 2014. The beneficial fungus Piriformospora indica protects Arabidopsis from Verticillium dahlia infection by downregulation plant defense responses. BMC Plant Biology, 14 (1): 268. https://doi.org/10.1186/s12870-014-0268-5
Verma, S., A. Varma, K.H. Rexer, A. Hassel, G. Kost, A. Sarbhoy, P. Bisen, B. Bütehorn & P. Franken, 1998. Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus. Mycologia, 90 (5): 896-903. https://doi.org/10.1080/00275514.1998.12026983
Yadav, R.K., M.K. Tripathi, S. Tiwari, N. Tripathi, R. Asati, V. Patel, R.S. Sikarwar & D.K. Payasi, 2023. Breeding and genomic approaches towards development of fusarium wilt resistance in chickpea. Life, 13 (4): 988. https://doi.org/10.3390/life13040988

Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Sercan Pazarlar
0000-0002-3768-4923
Türkiye

Eray Şimşek ^*
0000-0003-4984-4223
Türkiye

Early Pub Date

December 16, 2024

Publication Date

December 16, 2024

Submission Date

March 30, 2024

Acceptance Date

October 18, 2024

Published in Issue

Year 2024 Volume: 61 Number: 4

DOI

https://doi.org/10.20289/zfdergi.1461733

IZ

https://izlik.org/JA53NR67ZM

APA

Pazarlar, S., & Şimşek, E. (2024). The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University, 61(4), 449-459. https://doi.org/10.20289/zfdergi.1461733

AMA

1.Pazarlar S, Şimşek E. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 2024;61(4):449-459. doi:10.20289/zfdergi.1461733

Chicago

Pazarlar, Sercan, and Eray Şimşek. 2024. “The Endophytic Fungus Serendipita Indica Colonization Protects Chickpea Plants Against Fusarium Wilt Disease”. Journal of Agriculture Faculty of Ege University 61 (4): 449-59. https://doi.org/10.20289/zfdergi.1461733.

EndNote

Pazarlar S, Şimşek E (December 1, 2024) The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University 61 4 449–459.

IEEE

[1]S. Pazarlar and E. Şimşek, “The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease”, Journal of Agriculture Faculty of Ege University, vol. 61, no. 4, pp. 449–459, Dec. 2024, doi: 10.20289/zfdergi.1461733.

ISNAD

Pazarlar, Sercan - Şimşek, Eray. “The Endophytic Fungus Serendipita Indica Colonization Protects Chickpea Plants Against Fusarium Wilt Disease”. Journal of Agriculture Faculty of Ege University 61/4 (December 1, 2024): 449-459. https://doi.org/10.20289/zfdergi.1461733.

JAMA

1.Pazarlar S, Şimşek E. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 2024;61:449–459.

MLA

Pazarlar, Sercan, and Eray Şimşek. “The Endophytic Fungus Serendipita Indica Colonization Protects Chickpea Plants Against Fusarium Wilt Disease”. Journal of Agriculture Faculty of Ege University, vol. 61, no. 4, Dec. 2024, pp. 449-5, doi:10.20289/zfdergi.1461733.

Vancouver

1.Sercan Pazarlar, Eray Şimşek. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 2024 Dec. 1;61(4):449-5. doi:10.20289/zfdergi.1461733

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