The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease

Sercan Pazarlar; Eray Şimşek

doi:10.20289/zfdergi.1461733

EN TR

The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease

Öz

Objective: The objective of this study is to assess the efficacy of Serendipita indica, a basidiomycete endophyte, as a biological control agent against Fusarium wilt disease caused by Fusarium oxysporum f. sp. ciceris in disease susceptible chickpea cultivar JG62. Material and Methods: Chlamydospores of Serendipita indica were applied to the roots of the germinated JG62 variety, followed by inoculation with Fusarium oxysporum f. sp. ciceris race 5 one week after the application. Disease severity and plant fresh weight were measured 25 days after inoculation. The colonization pattern of Serendipita indica and Fusarium oxysporum f. sp. ciceris were monitored by quantifying fungal DNA using qPCR over time. The antagonistic interactions between the fungi were determined using the dual-culture method. Results: Serendipita indica successfully colonized the chickpea roots leading to a decrease in biomass of Fusarium oxysporum f. sp. ciceris in the roots and diminished the overall symptoms such as wilting and yellowing caused by Fusarium infection. Besides, direct antagonistic effect of Serendipita indica was found against Fusarium in vivo conditions. Conclusion: These results indicate the potential of Serendipita indica as a biological control agent in developing sustainable strategies for managing Fusarium wilt of chickpea.

Anahtar Kelimeler

Biocontrol agents, fungal endophyte, Fusarium oxysporum f. sp. ciceris, plant defense, Serendipita indica

Endofitik Fungus Serendipita indica kolonizasyonu nohut bitkilerini Fusarium solgunluğu hastalığına karşı korumaktadır

Öz

Amaç: Bu çalışmanın amacı, nohutlarda Fusarium oxysporum f. sp. ciceris tarafından kaynaklanan Fusarium solgunluğu hastalığına karşı biyolojik kontrol ajanı olarak Serendipita indica adlı bir basidiomycete endofit fungusun etkinliğini değerlendirmektir. Materyal ve Yöntem: Serendipita indica klamidosporları çimlendirilen JG62 çeşidinin köklerine uygulanmış ve uygulamadan 1 hafta sonra Fusarium oxysporum f. sp. ciceris ırk 5 inokulasyonu yapılmıştır. Hastalık şiddeti ve bitki yaş ağırlığı inokulasyondan 25 gün sonra ölçülmüştür., Serendipita indica ve Fusarium oxysporum f. sp. ciceris kolonizasyonu fungal DNA miktarının zamana bağlı olarak qPCR ile belirlenmesiyle izlenmiştir. Funguslar arasındaki antagonistik etkileşimler ikili-kültür yöntemi kullanılarak belirlenmiştir. Araştırma Bulguları: Serendipita indica başarılı bir şekilde nohut köklerini işgal ederek köklerdeki Fusarium oxysporum f. sp. ciceris biyomasının azalmasına ve Fusarium enfeksiyonu tarafından neden olan solgunluk ve sararma gibi belirtilerin azalmasına neden olmuştur. Ayrıca, Serendipita indica'nın Fusarium solgunluğu etmenine karşı doğrudan antagonistik etkisi bulunmuştur. Sonuç: Bu sonuçlar, nohutta Fusarium solgunluğuna karşı sürdürülebilir stratejiler geliştirmede Serendipita indica'nın biyolojik kontrol ajanı olarak potansiyelini göstermektedir.

Anahtar Kelimeler

Biyolojik kontrol, fungal endofit, Fusarium oxysporum f.sp. ciceris, bitki savunması ajanı, Serendipita indica

Teşekkür

The authors would like to thank Assoc. Prof. Dr. Abdullah Kahraman (Harran University, Türkiye) for providing Fusarium disease susceptible chickpea variety and Prof. Dr. Canan Can (Gaziantep University, Türkiye) for providing Fusarium oxysporum f.sp. ciceris (race 5) isolate. The authors also thank Dr. Jafargholi Imani (Justus Liebig University Giessen, Research Centre for BioSystems, Land Use and Nutrition, Institute of Phytopathology, Giessen, Germany) for providing S. indica strain.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Fitopatoloji

Bölüm

Araştırma Makalesi

Yazarlar

Sercan Pazarlar
0000-0002-3768-4923
Türkiye

Eray Şimşek ^*
0000-0003-4984-4223
Türkiye

Erken Görünüm Tarihi

16 Aralık 2024

Yayımlanma Tarihi

16 Aralık 2024

Gönderilme Tarihi

30 Mart 2024

Kabul Tarihi

18 Ekim 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 61 Sayı: 4

DOI

https://doi.org/10.20289/zfdergi.1461733

IZ

https://izlik.org/JA53NR67ZM

APA

Pazarlar, S., & Şimşek, E. (2024). The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University, 61(4), 449-459. https://doi.org/10.20289/zfdergi.1461733

AMA

1.Pazarlar S, Şimşek E. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 2024;61(4):449-459. doi:10.20289/zfdergi.1461733

Chicago

Pazarlar, Sercan, ve Eray Şimşek. 2024. “The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease”. Journal of Agriculture Faculty of Ege University 61 (4): 449-59. https://doi.org/10.20289/zfdergi.1461733.

EndNote

Pazarlar S, Şimşek E (01 Aralık 2024) The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University 61 4 449–459.

IEEE

[1]S. Pazarlar ve E. Şimşek, “The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease”, Journal of Agriculture Faculty of Ege University, c. 61, sy 4, ss. 449–459, Ara. 2024, doi: 10.20289/zfdergi.1461733.

ISNAD

Pazarlar, Sercan - Şimşek, Eray. “The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease”. Journal of Agriculture Faculty of Ege University 61/4 (01 Aralık 2024): 449-459. https://doi.org/10.20289/zfdergi.1461733.

JAMA

1.Pazarlar S, Şimşek E. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 2024;61:449–459.

MLA

Pazarlar, Sercan, ve Eray Şimşek. “The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease”. Journal of Agriculture Faculty of Ege University, c. 61, sy 4, Aralık 2024, ss. 449-5, doi:10.20289/zfdergi.1461733.

Vancouver

1.Sercan Pazarlar, Eray Şimşek. The endophytic fungus Serendipita indica colonization protects chickpea plants against Fusarium wilt disease. Journal of Agriculture Faculty of Ege University. 01 Aralık 2024;61(4):449-5. doi:10.20289/zfdergi.1461733