Research Article

The effects of different concentrations of growth regulators on the in vitro multiplication of potato (Solanum tuberosum L.)

Volume: 62 Number: 3 September 18, 2025
TR EN

The effects of different concentrations of growth regulators on the in vitro multiplication of potato (Solanum tuberosum L.)

Abstract

Objective: This research aims to study the effect of kinetin and NAA concentration interaction on the growth and multiplication of potato planlets (Solanum tuberosum L.) in vitro. Material and Methods: This research was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Brawijaya University from December 2023 to March 2024. The research was conducted in a randomized group design with two factors. The first factor is the GR Naphthalene Acetic Acid (NAA) with 4 concentration levels, 0 ppm, 0.25 ppm, 0.50 ppm and 0.75 ppm. The second factor is Kinetin with 4 concentration levels, 0 ppm, 2.5 ppm, 5 ppm and 7.5 ppm. Results: There is an interaction in the variable number of shoots, number of leaves, number of roots, planlet height and shoot emergence time. The number of shoots and the number of leaves have linear results or directly proportional to the growth of potato planlets. The addition of kinetin and NAA with the highest level of 7.5 ppm Kinetin and 0.75 ppm NAA can reduce the results on all observation variables. Conclusion The addition of Kinetin 7.5 ppm and NAA 0.25 ppm interacted and produced higher growth in number of shoots (0.46%), number of leaves (0.27%) and number of roots of potato planlets (0.43%) compared to without the addition of kinetin and without the addition of NAA.

Keywords

Growth regulators , in vitro , kinetin , multiplication , Naphthalene Acetic Acid (NAA) , potato planlets

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APA
Karyawati, A. S., & Maulidi, R. N. (2025). The effects of different concentrations of growth regulators on the in vitro multiplication of potato (Solanum tuberosum L.). Journal of Agriculture Faculty of Ege University, 62(3), 343-354. https://doi.org/10.20289/zfdergi.1539199