The effects of different concentrations of growth regulators on the in vitro multiplication of potato (Solanum tuberosum L.)
Öz
Objective: This research aims to study the effect of kinetin and NAA concentration interaction on the growth and multiplication of potato planlets (Solanum tuberosum L.) in vitro.
Material and Methods: This research was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Brawijaya University from December 2023 to March 2024. The research was conducted in a randomized group design with two factors. The first factor is the GR Naphthalene Acetic Acid (NAA) with 4 concentration levels, 0 ppm, 0.25 ppm, 0.50 ppm and 0.75 ppm. The second factor is Kinetin with 4 concentration levels, 0 ppm, 2.5 ppm, 5 ppm and 7.5 ppm.
Results: There is an interaction in the variable number of shoots, number of leaves, number of roots, planlet height and shoot emergence time. The number of shoots and the number of leaves have linear results or directly proportional to the growth of potato planlets. The addition of kinetin and NAA with the highest level of 7.5 ppm Kinetin and 0.75 ppm NAA can reduce the results on all observation variables.
Conclusion The addition of Kinetin 7.5 ppm and NAA 0.25 ppm interacted and produced higher growth in number of shoots (0.46%), number of leaves (0.27%) and number of roots of potato planlets (0.43%) compared to without the addition of kinetin and without the addition of NAA.
Anahtar Kelimeler
Growth regulators, in vitro, kinetin, multiplication, Naphthalene Acetic Acid (NAA), potato planlets
Destekleyen Kurum
University of Brawijaya
Proje Numarası
01
Etik Beyan
Hereby declare that it is true that we have conducted research and made the journal.
Teşekkür
Thankyou for your attention and contribution
Kaynakça
- Aiman, M., A. Abdullah & S. Numba, 2022. Daya multiplikasi tunas kentang secara in vitro dalam media dasar murashige and skoog (ms) dengan penambahan suplemen ekstrak tomat dan air kelapa. Jurnal Agrotekmas, 3 (1): 21-29.
- Arimarsetiowati, R. & F. Ardiyani, 2012. Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan somatik embriogenesis. Jurnal Pelita Perkebunan, 28 (2): 82-90.
- Central Bureau of Statistics, 2022. Vegetable Crop Production 2021-2023. (Web page: https://www.bps.go.id/id/statistics-table/2/NjEjMg==/produksi-tanaman-sayuran.html) (Date accessed: October 2023).
- Directorate General of Horticulture Ministry of Agriculture, 2023. Fixed Figures for Horticulture in 2022. Directorate General of Horticulture Ministry of Agriculture Jakarta, 63 pp.
- Husna, A. U., L. A. M. Siregar & Y. Husni, 2014. Pertumbuhan dan perkembangan nodus kentang (Solanum tuberosum L.) akibat modifikasi konsentrasi sukrosa dan penambahan 2-ısopenteniladenina secara in vitro. Jurnal Online Agroekoteknologi, 2 (3): 997-1003.
- Khalid, W., M. Z. Khalid, A. Aziz, A. Tariq, A. Ikram, M. Rehan, S. Younas, A. Bashir & A. Fatima, 2021. Nutritional composition and health benefits. Consortium, 5: 7-16. https://doi.org/10.1201/9781003105947-4
- Lestari, F. W., E. Suminar & S. Mubarok, 2018. Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi bap dan naa yang berbeda. Jurnal Agro, 5 (1): 66-75. https://doi.org/10.15575/1348
- Mehbub, H., A. Akter, M. A. Akter, M. S. H. Mandal, M. A. Hoque, M. Tuleja & H. Mehraj, 2022. Tissue culture ın ornamentals: cultivation factors, propagation techniques, and ıts application. Journal of Plants, 11 (23): 1-33. https://doi.org/10.3390/plants11233208
- Nascimeto, W. B., D. B. Mankowska & K. Zarzynska, 2020. Challenges ın the production of high-quality seed potatoes (Solanum tuberosum L.) in the tropics and subtropics. Journal Agronomy, 10 (2): 1-15. https://doi.org/10.3390/agronomy10020260
- Ni’mah, F., E. Ratnasari & L. S. Shootsipramana, 2012. Pengaruh pemberian berbagai kombinasi konsentrasi sukrosa dan kinetin terhadap ınduksi umbi mikro kentang (Solanum tuberosum L.) kultivar granola kembang secara ın-vitro. Jurnal Lentera Bio, 1 (1): 41-48.
