Determination and prevalence of Tomato spotted wilt virus (TSWV) and Cucumber mosaic virus (CMV) in pepper production areas in Demre district of Antalya
Abstract
Objective: In this study, it was aimed to serological determination of the presence of TSWV and CMV on pepper plants grown in the greenhouses in Kumluca district where Antalya pepper production is carried out intensely and compare the accuracy of the criteria determining that positive applied in by DAS-ELISA with RT-PCR method.
Material and Methods: Samples were collected from virus-like symptoms showing plants by conducting surveys in greenhouse pepper production areas in the Demre district of Antalya province. Samples were subjected to DAS-ELISA test using monoclonal and /or polyclonal antibodies specific for CMV and TSWV. RT-PCR tests were applied to some positive samples which have different absorbance values in the ELISA reader by using specific primers.
Results: According to the results of the DAS-ELISA method, 65 of the 82 samples (79%) were found to have at least one virus. The number of samples infected only with CMV was 10 (12%) and the number of samples infected only with TSWV was 47 (57%). In 8 samples (10%) were found to have both TSWV and CMV mixed infections. The presence of the factors in the samples which have positive absorbance values in the ELISA reader were compared by RT-PCR method and the presence of the factors was confirmed.
Conclusion: It was determined that TSWV factor was found more in pepper samples and CMV factor did not show much prevalence.
Keywords
Cucumber mosaic virus (CMV) , DAS-ELISA , RT-PCR , Tomato spotted wilt virus (TSWV)
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