Research Article
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Effects of Lutein, Lycopene and Vitamin E Supplementation to Diet on Performance, Egg Quality and Oxidative Stability in Laying Hens

Year 2016, Volume: 53 Issue: 3, 317 - 324, 27.09.2016
https://doi.org/10.20289/zfdergi.390080

Abstract

In this study, it was examined the effects of lycopene, lutein and
vitamin E supplementation to layer diet on performance, egg quality and
oxidative stability. A total of 250 layer hens (Supernick-White) at the age of
28 weeks were used. Hens were randomly allocated to five groups consisting of
five replicates (10 hens). The diet of control group (C) was not supplemented
any carotenoids. Diets of the other four experimental groups were added
lycopene (LK, 250 mg/kg), lutein (LT, 500 mg/kg), lycopene+lutein (LK+LT,
125+250 mg/kg) and lycopene+lutein+vitamin E (LK+L+E, 125+250+200 mg/kg).
Supplementation of lycopene, lutein and vitamin E to diet did not affect body
weight and feed intake of hens. In terms of feed conversion ratio, no
significant differences were occurred between C and other experimental groups.
However, feed conversion ratio significantly improved in LK and LK+LT groups
than LT and LK+LT+E groups. Egg yield increased in LK and LK+LT groups but
decreased in LK+LT+E group. Egg quality criteria were not affected by lutein, lycopene
and vitamin E supplementation except yolk color. Carotenoids supplementation
significantly decreased L* values whereas increased Roche Color Fan score and
the values of a* and b*. The lycopene and lutein contens of egg yolk
significantly rised. Compared with the control group, the MDA level of egg yolk
showed significant reduction in groups supplemented carotenoids only in 15th
day.

References

  • Açıkgöz, Z. ve S. Soycan Önenç. 2006. Fonksiyonel yumurta üretimi. Hayvansal Üretim, 47 (1): 36-46.
  • Akdemir, F., C. Orhan, N. Sahin, K. Sahin, A. Hayirli. 2012. Tomato powder in laying hen diets effects on concentrations of yolk carotenoids and lipid peroxidation. British Poultry Science, 53 (5): 675-680.
  • Altan, Ö. 2015. YUMURTA Oluşumu, Kalitesi ve Biyoaktif Komponentleri. ISBN:978-605-84400-0-5, Ege Üniversitesi Basımevi, İZMİR.
  • Anonim. 2004. Yem Analiz Metodları (Tebliğ no:2004/33). Resmi Gazete, 02.09.2004, Ankara, Sayı: 25571.
  • Arvanitoyannis, I.S. and M.V. Houwelingen-Koukaliaroglou. 2005. Funtional foods: A survey of health claims, pros and cons, and current legislation. Critical Reviews in Food Science and Nutrition, 45: 385-404.
  • Card, L.E. and M.C. Nesheim. 1972. Poultry Production. 11thed. Lea and Febiger, Philadelpia.
  • Çalışlar, S. ve G. Uygur. 2010. Effect of dry tomato pulp on egg yolk pigmentation and some egg yield charecteristics of laying hens. Journal of Animal and Veterinary Advances, 9(1):96-98.
  • Doğan, N. 2007. Rasyon likopen içeriğinin yumurtacı tavuklarda yumurta verimi, yumurta kalite özellikleri ve bazı kan parametreleri üzerine etkileri. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi.
  • Gianetti J., R. Pedrinelli, R. Petrucci, G. Lazzerini, M. De Caterina, G. Bellomo and R. De Caterina. 2002. Inverse association between carotid intima-media thickness and the antioxidant lycopene in atherosclerosis. American Heart Journal, 143(3): 467-74.
  • Giovannucci, E. 1999. Tomatoes, tomato-based products, lycopene, and cancer: Review of the epidemiologic literature. Journal of the National Cancer Institute, 91 (4): 317-331
  • Giovannucci, E., A. Ascherio, E.B. Rimm, M.J. Stampfer, G.A. Colditz and W.C. Willett. 1995. Intake of carotenoids and retinol in relation to risk of prostate cancer. Journal of the National Cancer Institute, 87(23): 1767-1776.
  • Galobart, J., R. Sala, X. Rincón-Carruyo, E.G. Manzanillla, B. Vilà and J. Gasa, 2004. Egg yolk color as affected by saponification of different natural pigmenting sources. The Journal of Applied Poultry Rresearch, 13: 328-334.
  • Jafari, M., R. Pirmohammadi and V. Bampidis. 2006. The use of dried tomato pulp in diets of laying hens. International Journal of Poultry Science, 5(7): 618-622.
  • Kırkpınar, F. 1993. Bazı doğal ve sentetik renk maddelerinin yumurta sarısının rengi ve verimle ilgili çeşitli kriterler üzerine etkileri. E.Ü. Fen Bilimleri Enstitüsü Zootekni Anabilim Dalı Doktora Tezi, İzmir.
  • Konings, E.J.M. and H.H.S. Roomans. 1997. Evaluation and validation of ann LC method for the analysis of carotenoids in vegetables and fruit. Food Chemistry, 59: 599-603.
  • Korhonen, H. 2002. Technology options for new nutritional concept. International Journal of Dairy Tecnology, 55(2): 79-87.
  • Landrum, J. and R. Bone. 2001. Lutein, zeaxanthin, and the macular pigment. Archives Biochemistry Biophysics, 385:28-40.
  • Leeson, S. and L. Caston. 2004. Enrichment of eggs with lutein. Poultry Science, 83: 1709- 1712.
  • Leeson, S., L. Caston, and H. Namkung. 2007. Effect of dietary lutein and flax on performance, egg composition and liver status of laying hens. Canadian Journal of Animal Science, 87: 365–372.
  • Lokaewmanee, K., K., Yamauchi, T. Komori and K. Saito. 2011. Enhancement of yolk color in raw and boiled egg yolk with lutein from marigold flower meal and marigold flower extract. Journal of Poultrry Science, 48(1): 25-32.
  • Lykkesfeldt, L. 2001. Determination of malondialdehyde as thiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: Comparison with ultraviolet-visible spectrophotometry. Clinical Chemistry, 47: 1725-1727.
  • Moeller, S.M., N. Parekh, L. Tinker, C. Ritenbaugh, B. Blodi, R.B. Wallace and J.A. Mares. 2006. Associations between intermediate age-related macular degeneration and lutein and zeaxanthin in the carotenoids in age-related eye disease study (CAREDS): Ancillary study of the women's health initiative. Archives of Ophthalmology, 124(8): 1151-62.
  • Naumann, C. and R. Bassler. 1993. Methodenbuch, Band III. Die Chemische Untersuchung von Futtermitteln. VDLUFA-Verlag, Darmstadt, Germany.
  • Olson, J.B., N.E. Ward and E.A. Koutsos. 2008. Lycopene incorporation into egg yolk and effects on laying hen immune function. Poultry Science, 87: 2573-2580.
  • Paas, E. and G. Pierce. 2002. An Introduction to Functional Foods, Nutraceuticals and Natural Health Products. National Centre for Agri-Food Research in Medicine. http://www.sbrc.ca/ncarm/ introfuncfoods.htm.
  • Rao A.V. and A. Ali. 2007. Biologically active phytochemicals in human health: Lycopene. International Journal of Food Properties, 10(2): 279-288.
  • Rowghani, E., A. Maddahian and M. Arab Abousadi. 2006. Effects of addition of marigold fiower, safflower petals, red peper on egg-yolk color and egg production in laying hens. Pakistan Journal of Biological Sciences, 9(7): 1333-1337.
  • SAS, 1999. System for Windows, Release 6. SAS Institute Inc., Cary, NC.
  • Sahin, K., F. Akdemir, C. Orhan, O. Küçük, A. Hayırlı and K. Sahin. 2008. Lycopene-enriched quail egg as functional food for humans. Food Research International, 41: 295-300.
  • Sahin, K., M. Onderci, N. Sahin, M.F. Gursu, F. Khachik, and O. Kucuk. 2006. Effcets of lycopene supplementation on antioxidant status, oxidative stress, performance and carcass characteristics in heat-stressed Japanese quail. Journal of Thermal Biology, 31: 307-312. Yannakopoulos, A.L., A.S. Tserveni-Gousi and E.V. Christiaki. 1992. Effect of locally produced tomato meal on the performance and the egg quality of laying hens. Animal Feed Science and Technology, 36(1-2):53-57.

Yumurtacı Tavuklarda Yeme Likopen, Lutein ve Vitamin E İlavesinin Performans, Yumurta Kalitesi ve Oksidatif Stabilite Üzerine Etkileri

Year 2016, Volume: 53 Issue: 3, 317 - 324, 27.09.2016
https://doi.org/10.20289/zfdergi.390080

Abstract








 






Çalışmada,
yumurta tavukların yemine likopen, lutein ve vitamin E ilavesinin performans,
yumurta kalitesi ve oksidatif stablite üzerine etkileri incelenmiştir. Toplam
28 hafta yaşında 250 adet (Supernick-Beyaz) yumurtacı tavuk kullanılmıştır.
Tavuklar rastgele her biri 5 tekerrürden (10 tavuk) oluşan   5
muamele grubuna ayrılmıştır. Kontrol (K) grubu yemine renk maddesi ilavesi
yapılmamıştır. Diğer dört muamele grubunun yemine likopen (LK, 250 mg/kg),
lutein (LT, 500 mg/kg), likopen+lutein (LK+LT, 125+250 mg/kg) ve
likopen+lutein+vitamin E (LK+LT+E, 125+250+200 mg/kg) ilavesi yapılmıştır. Yeme
likopen, lutein ve vitamin E ilave edilmesi tavukların canlı ağırlığını, yem
tüketimi etkilememiştir. Yemden yararlanma bakımından K ile diğer muamale
grupları arasında önemli düzeyde farklılıklar oluşmamıştır. Ancak, LK ve LK+LT
gruplarında LT ve LK+LT+E gruplarına göre yemden yararlanma iyileşmiştir.
Yumurta verimi LK ve LK+LT gruplarında önemli düzeyde artmış ancak LK+LT+E grubunda
azalmıştır. Sarısı rengi hariç yumurta kalite kriterleri likopen, lutein ve
vitamin E ilavesinden etkilenmemiştir. Renk maddesi ilavesi önemli düzeyde L*
değerini azaltırken Roche Renk Yelpazesi puanı ile a* ve b* değerlerini
artırmıştır. Yumurta sarısı lutein ve likopen içeriği önemli düzeyde
yükselmiştir.  Kontrol grubu ile
karşılaştırıldığında, yumurta sarısı MDA düzeyi renk maddesi ilave edilen
gruplarda sadece 15.günde önemli düzeyde azalma göstermiştir.

References

  • Açıkgöz, Z. ve S. Soycan Önenç. 2006. Fonksiyonel yumurta üretimi. Hayvansal Üretim, 47 (1): 36-46.
  • Akdemir, F., C. Orhan, N. Sahin, K. Sahin, A. Hayirli. 2012. Tomato powder in laying hen diets effects on concentrations of yolk carotenoids and lipid peroxidation. British Poultry Science, 53 (5): 675-680.
  • Altan, Ö. 2015. YUMURTA Oluşumu, Kalitesi ve Biyoaktif Komponentleri. ISBN:978-605-84400-0-5, Ege Üniversitesi Basımevi, İZMİR.
  • Anonim. 2004. Yem Analiz Metodları (Tebliğ no:2004/33). Resmi Gazete, 02.09.2004, Ankara, Sayı: 25571.
  • Arvanitoyannis, I.S. and M.V. Houwelingen-Koukaliaroglou. 2005. Funtional foods: A survey of health claims, pros and cons, and current legislation. Critical Reviews in Food Science and Nutrition, 45: 385-404.
  • Card, L.E. and M.C. Nesheim. 1972. Poultry Production. 11thed. Lea and Febiger, Philadelpia.
  • Çalışlar, S. ve G. Uygur. 2010. Effect of dry tomato pulp on egg yolk pigmentation and some egg yield charecteristics of laying hens. Journal of Animal and Veterinary Advances, 9(1):96-98.
  • Doğan, N. 2007. Rasyon likopen içeriğinin yumurtacı tavuklarda yumurta verimi, yumurta kalite özellikleri ve bazı kan parametreleri üzerine etkileri. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi.
  • Gianetti J., R. Pedrinelli, R. Petrucci, G. Lazzerini, M. De Caterina, G. Bellomo and R. De Caterina. 2002. Inverse association between carotid intima-media thickness and the antioxidant lycopene in atherosclerosis. American Heart Journal, 143(3): 467-74.
  • Giovannucci, E. 1999. Tomatoes, tomato-based products, lycopene, and cancer: Review of the epidemiologic literature. Journal of the National Cancer Institute, 91 (4): 317-331
  • Giovannucci, E., A. Ascherio, E.B. Rimm, M.J. Stampfer, G.A. Colditz and W.C. Willett. 1995. Intake of carotenoids and retinol in relation to risk of prostate cancer. Journal of the National Cancer Institute, 87(23): 1767-1776.
  • Galobart, J., R. Sala, X. Rincón-Carruyo, E.G. Manzanillla, B. Vilà and J. Gasa, 2004. Egg yolk color as affected by saponification of different natural pigmenting sources. The Journal of Applied Poultry Rresearch, 13: 328-334.
  • Jafari, M., R. Pirmohammadi and V. Bampidis. 2006. The use of dried tomato pulp in diets of laying hens. International Journal of Poultry Science, 5(7): 618-622.
  • Kırkpınar, F. 1993. Bazı doğal ve sentetik renk maddelerinin yumurta sarısının rengi ve verimle ilgili çeşitli kriterler üzerine etkileri. E.Ü. Fen Bilimleri Enstitüsü Zootekni Anabilim Dalı Doktora Tezi, İzmir.
  • Konings, E.J.M. and H.H.S. Roomans. 1997. Evaluation and validation of ann LC method for the analysis of carotenoids in vegetables and fruit. Food Chemistry, 59: 599-603.
  • Korhonen, H. 2002. Technology options for new nutritional concept. International Journal of Dairy Tecnology, 55(2): 79-87.
  • Landrum, J. and R. Bone. 2001. Lutein, zeaxanthin, and the macular pigment. Archives Biochemistry Biophysics, 385:28-40.
  • Leeson, S. and L. Caston. 2004. Enrichment of eggs with lutein. Poultry Science, 83: 1709- 1712.
  • Leeson, S., L. Caston, and H. Namkung. 2007. Effect of dietary lutein and flax on performance, egg composition and liver status of laying hens. Canadian Journal of Animal Science, 87: 365–372.
  • Lokaewmanee, K., K., Yamauchi, T. Komori and K. Saito. 2011. Enhancement of yolk color in raw and boiled egg yolk with lutein from marigold flower meal and marigold flower extract. Journal of Poultrry Science, 48(1): 25-32.
  • Lykkesfeldt, L. 2001. Determination of malondialdehyde as thiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: Comparison with ultraviolet-visible spectrophotometry. Clinical Chemistry, 47: 1725-1727.
  • Moeller, S.M., N. Parekh, L. Tinker, C. Ritenbaugh, B. Blodi, R.B. Wallace and J.A. Mares. 2006. Associations between intermediate age-related macular degeneration and lutein and zeaxanthin in the carotenoids in age-related eye disease study (CAREDS): Ancillary study of the women's health initiative. Archives of Ophthalmology, 124(8): 1151-62.
  • Naumann, C. and R. Bassler. 1993. Methodenbuch, Band III. Die Chemische Untersuchung von Futtermitteln. VDLUFA-Verlag, Darmstadt, Germany.
  • Olson, J.B., N.E. Ward and E.A. Koutsos. 2008. Lycopene incorporation into egg yolk and effects on laying hen immune function. Poultry Science, 87: 2573-2580.
  • Paas, E. and G. Pierce. 2002. An Introduction to Functional Foods, Nutraceuticals and Natural Health Products. National Centre for Agri-Food Research in Medicine. http://www.sbrc.ca/ncarm/ introfuncfoods.htm.
  • Rao A.V. and A. Ali. 2007. Biologically active phytochemicals in human health: Lycopene. International Journal of Food Properties, 10(2): 279-288.
  • Rowghani, E., A. Maddahian and M. Arab Abousadi. 2006. Effects of addition of marigold fiower, safflower petals, red peper on egg-yolk color and egg production in laying hens. Pakistan Journal of Biological Sciences, 9(7): 1333-1337.
  • SAS, 1999. System for Windows, Release 6. SAS Institute Inc., Cary, NC.
  • Sahin, K., F. Akdemir, C. Orhan, O. Küçük, A. Hayırlı and K. Sahin. 2008. Lycopene-enriched quail egg as functional food for humans. Food Research International, 41: 295-300.
  • Sahin, K., M. Onderci, N. Sahin, M.F. Gursu, F. Khachik, and O. Kucuk. 2006. Effcets of lycopene supplementation on antioxidant status, oxidative stress, performance and carcass characteristics in heat-stressed Japanese quail. Journal of Thermal Biology, 31: 307-312. Yannakopoulos, A.L., A.S. Tserveni-Gousi and E.V. Christiaki. 1992. Effect of locally produced tomato meal on the performance and the egg quality of laying hens. Animal Feed Science and Technology, 36(1-2):53-57.
There are 30 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Umut Ekiz This is me

Zümrüt Açıkgöz This is me

Publication Date September 27, 2016
Submission Date March 14, 2016
Acceptance Date May 2, 2016
Published in Issue Year 2016 Volume: 53 Issue: 3

Cite

APA Ekiz, U., & Açıkgöz, Z. (2016). Yumurtacı Tavuklarda Yeme Likopen, Lutein ve Vitamin E İlavesinin Performans, Yumurta Kalitesi ve Oksidatif Stabilite Üzerine Etkileri. Journal of Agriculture Faculty of Ege University, 53(3), 317-324. https://doi.org/10.20289/zfdergi.390080

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