Propagation of GF 677 (P. amygdalus x P. persica) Clone Rootstock in Tissue Culture by Shoot-tip Techique

Year 2017, Volume: 54 Issue: 4, 447 - 451, 27.12.2017

Serra Hepaksoy

https://doi.org/10.20289/zfdergi.386559

Cited By: 3

Abstract

Modified Murashige and Skoog (MS) nutrient medium was
used to propagate the GF 677 clone rootstock by shoot tip technique.  Survival ratio of shoot tips was differed
between 38% and 60% and increased by BAP concentration. The best multiplication
was obtained on a MS medium supplemented with 2.0 mg/l BAP, 0.1 mg/l NAA and
0.1 mg/l GA₃. In general multiplication ration was
increased when GA₃ added to medium. Rooting of in vitro
plantlets was better with NAA than IBA while there was no significant
difference between auxins. The highest rooting was obtained on half or full
strength MS medium containing 1.0 and 1.5 mg/l NAA or IBA. Half or full
strength MS medium were showed similar  affect on rooting.

Keywords

GF 677, in vitro, MS medium

GF 677 (P. amygdalus x P. persica) Klon Anacının Doku Kültüründe Sürgünucu Tekniği ile Çoğaltılması

Abstract

GF  677
klon anacının sürgün ucu tekniğiyle çoğaltılması amacıyla modifiye Murashige
Skoog (MS) besin ortamı kullanılmıştır. Sürgün uçlarının canlılık oranı %38 ile
% 60 arasında değişmiş ve BAP konsantrasyonu arttıkça canlılık oranı da
artmıştır. En iyi çoğalma 2.0 mg/l BAP + 0.1 mg/l NAA + 0.1 mg/l GA₃
içeren MS ortamından elde edilmiş, genel olarak besin ortamında GA₃
bulunmasının kardeşlenme sayısını arttırdığı tespit edilmiştir. In vitro bitkiciklerin köklenmesinde
NAA, IBA’e göre daha iyi sonuç vermekle birlikte, istatistiksel olarak önemli
farklılıklar bulunmamıştır. Besin ortamına 1.0 ve 1.5 mg/l NAA veya IBA ilave
edilmesi en yüksek köklenme oranlarını vermiştir. Tam veya yarı kuvvette MS
besin ortamının kullanılması köklenme üzerine benzer etki göstermiştir.

Keywords

GF 677, in vitro, MS ortamı

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