Search of Transcription Factors Involved in Grape Berry Ripening

Year 2007, Volume: 44 Issue: 1, 41 - 54, 01.03.2007

Birsen Çakır Rossitza Atanassova

Abstract

Keywords

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Üzümün Olgunlaşmasında Etkili Olan Transkripsiyon Faktörleri Üzerine Bir Araştırma

Abstract

Olgunlaşma sırasında, üzüm taneleri büyük miktarlarda hekzos biriktirirler (1M). Bu şeker birikimi şeker ve hekzos taşıyıcılarının aktivitesini gerektirir. Ben düşmeden önce ve sonra ekspresyonu ortaya çıkan bir hekzos taşıyıcısını kodlayan cDNA ve genomik klon laboratuvarda daha önceden izole edilmişti. VvHT1 promotörü, şekere cevap veren çeşitli elemanları bulundurmaktadır. Bu çalışmada, VvHT1 promotörüne bağlanabilecek düzenleyici proteinlerin izolasyonunu tanımlanmıştır. Bunun için, ben düşme aşamasındaki üzümlerden elde edilmiş RNA lardan bir cDNA kütüphanesi hazırlanmıştır. Maya hücrelerinde tek hibrid adı verilen teknik ile transkripsiyon faktörlerini kodlayan dokuz farklı cDNA izole edilmiş ve bu klonlar Genbankasına yatırılmıştır

Keywords

Vitis vinifera, olgunlaşma, hekzos taşıyıcısı, ekspresyonun düzenlenmesi

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